Abstract: The fermentation medium and conditions of Streptomyces canus sp. FIM0916 were optimizied for  producing amphomycin by single-factor and orthogonal test design. The optimized medium formula is 1.5% sucrose, 3.5% soybean meal, 0.1% L-histidine, 0.1% KNO₃ and 0.1% CaCO₃；the optimal fermentation conditions are as follows： filling volume is 120 mL for a 500 mL shake flask, shakers rotating speed is 250 r/min， inoculum size is 2%， temperature 28℃， and cultivation time of seed 54 h; the optimal fermentation time are 5 d. Under optimized conditions, the titer of amphomycin was 248% higher than that of control,  which laid the foundation for subsequent development on amphomycin.

Key words: amphomycin, single factor experiment, orthogonal experiment, fermentation conditions optimization

摘要： 本文采用单因素和正交试验设计，对链霉菌 Streptomyces canus sp. FIM-0916产安福霉素的发酵培养基配方及发酵条件进行了优化。优化后的最佳培养基配方为：蔗糖1.5%，黄豆粉1.0%，组氨酸0.1%，KNO3 0.1%， CaCO3 0.1%。最佳发酵条件为：种子菌龄54 h，装液量120 mL/500 mL，接种量2%，发酵温度28℃，摇床转速250 r/min；最佳发酵时间为5 d。在该优化条件下，安福霉素的发酵效价比对照提高248%，为安福霉素的后续开发奠定了基础。

关键词: 安福霉素, 单因素考察, 正交试验, 发酵条件优化