Abstract: Aminoglycoside antibiotics are a kinds of early discovered and applied clinical antibiotics. Although they have not completely disappeared from the market， along with the emergence of broad spectrum antibiotics with fewer side effects，its importance has diminished. At present，due to the infection caused by multidrug resistant (MDR) bacteria increased dramatically, as one of the few treatment options，aminoglycoside antibiotics are back to peoples vision，especially for Gram-negative bacterial infection. Although their resistance mechanism has been basically clear，the understanding of antibacterial mode is far from comprehensive. Facing more and more virtually untreatable infections, this kind of old antibiotics would have application potential to combat multi-drug resistant pathogens.

Key words: aminoglycoside antibiotics, multidrug-resistant, Gram-negative pathogens, antimicrobial mode