Curr. Biotech. ›› 2013, Vol. 3 ›› Issue (3): 218-222.DOI: 10.3969/j.issn.2095-2341.2013.0 .

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Expression and Optimized Inducing Conditions of Recombinant Oxalate Decarboxylase

WEI Cheng-yu, LIN Ri-hui, LIANG Yue, LONG Han, XUAN Jin-cai   

  1. Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, School of Marine Sciences and Biotechnology, Guangxi University for Nationalities, Nanning 530006, China
  • Received:2013-01-15 Online:2013-05-25 Published:2013-04-02

Abstract: The optimized inducing condition of recombinant oxalate decarboxylase derived from Bacillus subtilis was studied. The results showed that 960 U oxalate decarboxylase per liter was induced under the conditions as follows: initial pH at 8.0, the recombinant strain was induced by adding 0.4 mmol/L IPTG and 6 mmol/L MnCl2 after 3 h, and cultured for 10 h.

Key words: oxalate decarboxylase, induced expression, optimization