Curr. Biotech. ›› 2013, Vol. 3 ›› Issue (3): 179-184.DOI: 10.3969/j.issn.2095-2341.2013.0 .

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Research Progress of the Global Regulator IrrE in Deinococcus radiodurans

CHEN Zhen, ZHOU Zheng-fu, ZHANG Wei, CHEN Ming, SONG Yuan, LIN Min   

  1. 1.College of Biological Sciences, China Agricultural University, Beijing 100193, China;
    2.Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2013-03-13 Online:2013-05-25 Published:2013-04-20

耐辐射异常球菌全局调控蛋白IrrE的研究进展

陈震1,2,周正富2,张维2,陈明2,宋渊1,林敏2*   

  1. 1.中国农业大学生物学院， 北京 100193；
    2.中国农业科学院生物技术研究所， 北京 100081
  • 通讯作者: 林敏，研究员，博士，博士生导师，主要从事固氮微生物及特殊环境微生物的分子生物学和基因工程研究。E-mail:linmin57@vip.163.com
  • 作者简介:陈震，硕士研究生，研究方向抗逆微生物分子生物学。
  • 基金资助:

    国家自然科学基金项目（31070084）；农业部公益性行业科研专项（201103007）；国家转基因生物新品种培育重大专项（2013ZX08012-001;2013ZX08009-003）资助。

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Abstract: The unique global regulator IrrE, found in Deinococcus, can up-regulate the expression of DNA repair genes in response to DNA damage. 5 types of IrrE protein had been identified in different strains of Deinococcus, the results of sequence alignment and homology modeling suggest that they share a high identity in amino acid sequence and have the same conserved protein regions, which may indicate the similarity in their functions. Besides, when gene irrE expressed in E.coli and B. napus, the salt tolerance of both hosts can be significantly enhanced, which show a very promising future in application. This review introduced the basic traits of Deinococcus and the discovery, structure and related functions of the global regulator IrrE, then we analyzed and prospected its potential application.  

Key words: Deinococcus, global regulator IrrE, stress resistance

摘要： 全局调控蛋白IrrE是异常球菌属中所特有的一种DNA损伤修复调节因子，可以显著提高细胞受到损伤时各修复基因的表达。在目前已经完成测序的异常球菌中共发现7种不同来源的IrrE蛋白，经序列比对与同源建模，发现其氨基酸序列相似性较高且具有相同的保守结构域，这可能预示了其功能上的相似性。此外，irrE在大肠杆菌及油菜中表达后，能明显增强宿主的耐盐性，体现了较高应用价值。本综述介绍了异常球菌属及其全局调控蛋白IrrE的发现、结构与相关功能，分析与展望了该调控蛋白潜在的应用前景。

关键词: 耐辐射异常球菌, 全局调控蛋白IrrE, 胁迫抗性

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