Study on Screening of Odorant Degradation Enzyme Genes in Dioryctria sylvestrella and its Expression Level in Different Tissues

doi:10.19586/j.2095-2341.2025.0145

Current Biotechnology ›› 2025, Vol. 15 ›› Issue (6): 1003-1019.DOI: 10.19586/j.2095-2341.2025.0145

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Study on Screening of Odorant Degradation Enzyme Genes in Dioryctria sylvestrella and its Expression Level in Different Tissues

Kaipeng ZHANG¹^,²^,³(), Li WANG⁴, Li TANG⁴, Jing LI¹^,²^,³, Qi CHEN⁴()

^1.Jilin Provincial Forestry Biological Control Center Station，Jilin Provincial Academy of Forestry Sciences，Changchun 130033，China
^2.Key Laboratory of Animal Resources and Biodiversity of Changbai Mountain，Changchun 130033，China
^3.Jilin Province Main Forest Diseases and Insect Pests Monitoring，Epidemic Prevention Technology Innovation Center，Changchun 130033，China
^4.School of Life Sciences，Northeast Normal University，Changchun 130024，China

Received:2025-10-21 Accepted:2025-11-12 Online:2025-11-25 Published:2026-01-04
Contact: Qi CHEN

Abstract

Abstract:

This study aimed to identify， classify， and analyze the tissue expression patterns of odorant-degrading enzyme （ODE）-related genes in Dioryctria sylvestrella （Lepidoptera： Pyralidae）， thereby providing a theoretical basis for developing green and efficient control technologies against this pest. Four families of ODE-related genes were screened from the transcriptome data of female and male antennae of D. sylvestrella， including carboxylesterases （CXEs）， aldehyde oxidases （AOXs）， glutathione S-transferases （GSTs）， and cytochrome P450 monooxygenases （CYPs）. These genes were subjected to bioinformatics analysis， homologous sequence alignment， phylogenetic tree construction， and standardized naming. Candidate genes with relatively high FPKM values in antennae were selected for subsequent validation， and reverse transcription-quantitative polymerase chain reaction （RT-qPCR） was employed to analyze the tissue-specific expression profiles of the selected genes in different adult tissues. A total of 137 candidate ODE-related genes were identified， including 39 DsylCXEs， 6 DsylAOXs， 32 DsylGSTs， and 60 DsylCYPs. RT-qPCR results showed that 18 DsylODEs exhibited significantly higher expression levels in antennae than in other tissues. Among these antennae-enriched ODE genes， glutathione S-transferases （GSTs） and cytochrome P450 monooxygenases （CYPs） were the dominant families. This study is the first to screen and identify potential D. sylvestrella ODE genes with strong odor-degrading capabilities， which established a foundational framework for further investigating the odor degradation mechanisms involved in the olfactory recognition process of D. sylvestrella through sequence characterization and gene expression profiling.

Key words: Dioryctria sylvestrella, odorant degradation enzymes, transcriptome, RT-qPCR

摘要：

研究旨在对赤松梢斑螟（Dioryctria sylvestrella）气味降解酶（odorant-degrading enzyme, ODE）相关基因进行鉴定、分类及组织表达模式分析，以期为该害虫的绿色高效防治技术提供理论依据。在赤松梢斑螟雌、雄触角转录组数据中筛选出4种ODEs基因：羧酸酯酶（carboxylesterases, CXEs）、醛氧化酶（aldehyde oxidases, AOXs）、谷胱甘肽转移酶（glutathione S-transferases, GSTs）和细胞色素P450氧化酶（cytochrome P450s, CYPs），对这些基因进行生物信息学分析和同源建树、命名；选取触角中FPKM值较高的候选基因进行后续验证，并采用RT-qPCR技术分析所选基因在成虫不同组织中的特异表达情况。研究共鉴定出137个候选ODEs相关基因，包括39个DsylCXEs、6个DsylAOXs、32个DsylGSTs和60个DsylCYPs。RT-qPCR结果表明，18个DsylODEs在触角中的表达量显著高于其他组织。在赤松梢斑螟的触角中显著表达的ODEs基因类型主要为谷胱甘肽转移酶与细胞色素P450。研究首次筛选出了潜在的具有较强气味降解能力的赤松梢斑螟ODEs基因，为进一步探究赤松梢斑螟在嗅觉识别过程中的气味降解机制奠定了基础。

关键词: 赤松梢斑螟, 气味降解酶, 转录组, RT-qPCR

CLC Number:

Q965

Kaipeng ZHANG, Li WANG, Li TANG, Jing LI, Qi CHEN. Study on Screening of Odorant Degradation Enzyme Genes in Dioryctria sylvestrella and its Expression Level in Different Tissues[J]. Current Biotechnology, 2025, 15(6): 1003-1019.

张凯鹏, 王莉, 唐莉, 李婧, 陈琪. 赤松梢斑螟气味降解酶基因筛选及其在不同组织的表达差异研究[J]. 生物技术进展, 2025, 15(6): 1003-1019.

Figures/Tables 17

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基因名称	正向引物（5′→3′）	反向引物（5′→3′）
DsylCXE17	5′-CTTGGCCATGGTAAAGAAGC-3′	5′-TGAGAGGGTCTGGTCTTGCT-3′
DsylCXE20	5′-AGGAAAACTGCAAGGCAAGA-3′	5′-CAAATCTCAGCTCACCGACA-3′
DsylCXE30	5′-ATCCATGGAGGAGGCTTTTT-3′	5′-AGAGAAATCCGAGTGCCTCA-3′
DsylCXE32	5′-ATCACGGGTAGCGAAGATTG-3′	5′-TGCACCACCGTGAATAAAGA-3′
DsylCXE38	5′-CCTTCCGCTCTGTCGTTATC-3′	5′-GAGGAGTTGTTCAGGCTTGC-3′
DsylCXE39	5′-ATCTGGCACCAGGCAGTATC-3′	5′-ACATCTCCTCCCACACCTTG-3′
DsylAOX2	5′-CTCAAAGACTGTGGGGGAAA-3′	5′-TTGAGTTGTGGATGCTCTGC-3′
DsylAOX4	5′-ACCCGTACGCTGTAATCGAC-3′	5′-CTGCGGATAGCTGGAGAAAG-3′
DsylGST10	5′-GCCTTCGGCTATGACTTCAG-3′	5′-ATTTGTCCTCCTGCATGGTC-3′
DsylGST14	5′-GACGCCGGAATATTTGAAGA-3′	5′-TTCGGGTACAGGGAGTCATC-3′
DsylGST15	5′-AAATCCCCAGCATACAGTGC-3′	5′-TCTCTTCAGGGGGTCATTTG-3′
DsylGST20	5′-GGAAGATGAGGCTGTCAAGG-3′	5′-AGCAGCTAGGTGTCCGTTGT-3′
DsylGST23	5′-GGAAGATGAGGCTGTCAAGG-3′	5′-AGCAGCTAGGTGTCCGTTGT-3′
DsylGST30	5′-GGAAGATGAGGCTGTCAAGG-3′	5′-AGCAGCTAGGTGTCCGTTGT-3′
DsylCYP19	5′-CCGTGTGGGAGAAATGACTT-3′	5′-CATCACGATGTTTTCCGATG-3′
DsylCYP30	5′-GGATGGCTCGGTCACATACT-3′	5′-CTGCCGTAGCCAATGATTTT-3′
DsylCYP32	5′-AGGCCGAACATAACCACAAG-3′	5′-CGTTGAATTGCGAAGTTTGA-3′
DsylCYP36	5′-ACGCTAACATCTGGGGTCAC-3′	5′-ACCCCAATACTTCGGGTTTC-3′
DsylCYP37	5′-ATGCGTTTGTACCCTTCTCG-3′	5′-TCGGAAATTTCTCAGGATGG-3′
DsylCYP55	5′-CCCTGTGGGGCTGTTATAGA-3′	5′-CCGGAAAGAAATCTGGATGA-3′
DsylGAPDH	5′-TGCACAACTAACTGCCTTGC-3′	5′-TGGAAGCGGGAATTATGTTC-3′

基因名称	正向引物（5′→3′）	反向引物（5′→3′）
DsylCXE17	5′-CTTGGCCATGGTAAAGAAGC-3′	5′-TGAGAGGGTCTGGTCTTGCT-3′
DsylCXE20	5′-AGGAAAACTGCAAGGCAAGA-3′	5′-CAAATCTCAGCTCACCGACA-3′
DsylCXE30	5′-ATCCATGGAGGAGGCTTTTT-3′	5′-AGAGAAATCCGAGTGCCTCA-3′
DsylCXE32	5′-ATCACGGGTAGCGAAGATTG-3′	5′-TGCACCACCGTGAATAAAGA-3′
DsylCXE38	5′-CCTTCCGCTCTGTCGTTATC-3′	5′-GAGGAGTTGTTCAGGCTTGC-3′
DsylCXE39	5′-ATCTGGCACCAGGCAGTATC-3′	5′-ACATCTCCTCCCACACCTTG-3′
DsylAOX2	5′-CTCAAAGACTGTGGGGGAAA-3′	5′-TTGAGTTGTGGATGCTCTGC-3′
DsylAOX4	5′-ACCCGTACGCTGTAATCGAC-3′	5′-CTGCGGATAGCTGGAGAAAG-3′
DsylGST10	5′-GCCTTCGGCTATGACTTCAG-3′	5′-ATTTGTCCTCCTGCATGGTC-3′
DsylGST14	5′-GACGCCGGAATATTTGAAGA-3′	5′-TTCGGGTACAGGGAGTCATC-3′
DsylGST15	5′-AAATCCCCAGCATACAGTGC-3′	5′-TCTCTTCAGGGGGTCATTTG-3′
DsylGST20	5′-GGAAGATGAGGCTGTCAAGG-3′	5′-AGCAGCTAGGTGTCCGTTGT-3′
DsylGST23	5′-GGAAGATGAGGCTGTCAAGG-3′	5′-AGCAGCTAGGTGTCCGTTGT-3′
DsylGST30	5′-GGAAGATGAGGCTGTCAAGG-3′	5′-AGCAGCTAGGTGTCCGTTGT-3′
DsylCYP19	5′-CCGTGTGGGAGAAATGACTT-3′	5′-CATCACGATGTTTTCCGATG-3′
DsylCYP30	5′-GGATGGCTCGGTCACATACT-3′	5′-CTGCCGTAGCCAATGATTTT-3′
DsylCYP32	5′-AGGCCGAACATAACCACAAG-3′	5′-CGTTGAATTGCGAAGTTTGA-3′
DsylCYP36	5′-ACGCTAACATCTGGGGTCAC-3′	5′-ACCCCAATACTTCGGGTTTC-3′
DsylCYP37	5′-ATGCGTTTGTACCCTTCTCG-3′	5′-TCGGAAATTTCTCAGGATGG-3′
DsylCYP55	5′-CCCTGTGGGGCTGTTATAGA-3′	5′-CCGGAAAGAAATCTGGATGA-3′
DsylGAPDH	5′-TGCACAACTAACTGCCTTGC-3′	5′-TGGAAGCGGGAATTATGTTC-3′

基因名称	ORF长度/aa	是否有完整ORF	等电点pI	分子量/Da	最佳匹配登录号	覆盖率/%	相似度/%
DsylCXE1	72	否	10.26	8 104.48	QEA03473.1	100	68.49
DsylCXE2	132	否	9.14	13 850.71	WBO25687.1	100	97.74
DsylCXE3	69	否	8.24	7 756.10	WBO25685.1	100	98.57
DsylCXE4	569	是	5.26	64 239.31	WBO25673.1	98	92.79
DsylCXE5	146	否	4.20	16 401.57	QZC92208.1	100	97.28
DsylCXE6	543	是	6.61	60 808.34	QEA03460.1	100	82.18
DsylCXE7	135	否	9.92	15 497.94	QZC92238.1	87	91.53
DsylCXE8	469	是	4.79	52 641.05	QEA03454.1	100	86.78
DsylCXE9	269	是	5.52	30 268.99	WBO25687.1	100	94.42
DsylCXE10	65	否	11.79	7 408.83	QEA03454.1	100	86.36
DsylCXE11	340	是	6.05	39 054.24	WBO25685.1	99	80.47
DsylCXE12	202	是	4.12	22 542.12	WBO25688.1	82	94.55
DsylCXE13	209	是	10.34	22 976.37	XP_053605942.1	96	57.71
DsylCXE14	88	是	10.24	9 591.08	XP_028177914.1	90	56.96
DsylCXE15	243	是	9.30	26 289.89	XP_013200959.2	100	47.98
DsylCXE16	79	否	4.12	9 424.55	XP_037294223.1	100	65.00
DsylCXE17	476	是	5.41	53 153.99	XP_053608208.1	100	72.33
DsylCXE18	566	是	5.35	63 258.66	WNK22299.1	99	71.95
DsylCXE19	341	是	11.44	37 478.18	XP_013193107.1	100	91.52
DsylCXE20	527	是	4.84	59 161.58	XP_053606293.1	99	71.51
DsylCXE21	603	是	8.20	67 745.85	XP_052759019.1	99	64.77
DsylCXE22	550	是	7.00	60 391.19	QEA03464.1	100	79.01
DsylCXE23	546	是	6.58	62 718.06	XP_013190276.2	100	76.09
DsylCXE24	193	否	5.76	21 994.24	XP_013185431.1	98	72.25
DsylCXE25	316	是	4.47	35 776.48	QEA03461.1	100	75.32
DsylCXE26	330	是	5.96	38 378.05	XP_013183096.1	99	71.65
DsylCXE27	141	是	8.28	16 598.00	XP_053622034.1	100	70.92
DsylCXE28	532	是	6.11	60 081.62	XP_053604405.1	100	77.30
DsylCXE29	161	是	9.26	18 053.71	XP_013192630.1	99	77.50
DsylCXE30	543	是	6.31	61 038.43	WBO25679.1	83	87.64
DsylCXE31	78	否	3.98	9 245.44	XDZ19301.1	95	65.33
DsylCXE32	551	是	6.93	62 016.50	QEA03463.1	99	72.86
DsylCXE33	185	是	4.46	21 261.61	XP_013196080.2	100	75.14
DsylCXE34	340	是	8.27	38 859.50	XP_053603164.1	100	74.49
DsylCXE35	538	是	7.32	61 488.95	XP_053614450.1	100	73.65
DsylCXE36	150	否	8.99	16 865.08	WBO25686.1	100	96.69
DsylCXE37	531	是	5.14	59 761.73	XP_028168560.1	95	66.67
DsylCXE38	546	是	7.90	61 994.92	QEA03466.1	99	86.64
DsylCXE39	589	是	7.13	65 697.92	WBO25685.1	72	80.84

基因名称	ORF长度/aa	是否有完整ORF	等电点pI	分子量/Da	最佳匹配登录号	覆盖率/%	相似度/%
DsylCXE1	72	否	10.26	8 104.48	QEA03473.1	100	68.49
DsylCXE2	132	否	9.14	13 850.71	WBO25687.1	100	97.74
DsylCXE3	69	否	8.24	7 756.10	WBO25685.1	100	98.57
DsylCXE4	569	是	5.26	64 239.31	WBO25673.1	98	92.79
DsylCXE5	146	否	4.20	16 401.57	QZC92208.1	100	97.28
DsylCXE6	543	是	6.61	60 808.34	QEA03460.1	100	82.18
DsylCXE7	135	否	9.92	15 497.94	QZC92238.1	87	91.53
DsylCXE8	469	是	4.79	52 641.05	QEA03454.1	100	86.78
DsylCXE9	269	是	5.52	30 268.99	WBO25687.1	100	94.42
DsylCXE10	65	否	11.79	7 408.83	QEA03454.1	100	86.36
DsylCXE11	340	是	6.05	39 054.24	WBO25685.1	99	80.47
DsylCXE12	202	是	4.12	22 542.12	WBO25688.1	82	94.55
DsylCXE13	209	是	10.34	22 976.37	XP_053605942.1	96	57.71
DsylCXE14	88	是	10.24	9 591.08	XP_028177914.1	90	56.96
DsylCXE15	243	是	9.30	26 289.89	XP_013200959.2	100	47.98
DsylCXE16	79	否	4.12	9 424.55	XP_037294223.1	100	65.00
DsylCXE17	476	是	5.41	53 153.99	XP_053608208.1	100	72.33
DsylCXE18	566	是	5.35	63 258.66	WNK22299.1	99	71.95
DsylCXE19	341	是	11.44	37 478.18	XP_013193107.1	100	91.52
DsylCXE20	527	是	4.84	59 161.58	XP_053606293.1	99	71.51
DsylCXE21	603	是	8.20	67 745.85	XP_052759019.1	99	64.77
DsylCXE22	550	是	7.00	60 391.19	QEA03464.1	100	79.01
DsylCXE23	546	是	6.58	62 718.06	XP_013190276.2	100	76.09
DsylCXE24	193	否	5.76	21 994.24	XP_013185431.1	98	72.25
DsylCXE25	316	是	4.47	35 776.48	QEA03461.1	100	75.32
DsylCXE26	330	是	5.96	38 378.05	XP_013183096.1	99	71.65
DsylCXE27	141	是	8.28	16 598.00	XP_053622034.1	100	70.92
DsylCXE28	532	是	6.11	60 081.62	XP_053604405.1	100	77.30
DsylCXE29	161	是	9.26	18 053.71	XP_013192630.1	99	77.50
DsylCXE30	543	是	6.31	61 038.43	WBO25679.1	83	87.64
DsylCXE31	78	否	3.98	9 245.44	XDZ19301.1	95	65.33
DsylCXE32	551	是	6.93	62 016.50	QEA03463.1	99	72.86
DsylCXE33	185	是	4.46	21 261.61	XP_013196080.2	100	75.14
DsylCXE34	340	是	8.27	38 859.50	XP_053603164.1	100	74.49
DsylCXE35	538	是	7.32	61 488.95	XP_053614450.1	100	73.65
DsylCXE36	150	否	8.99	16 865.08	WBO25686.1	100	96.69
DsylCXE37	531	是	5.14	59 761.73	XP_028168560.1	95	66.67
DsylCXE38	546	是	7.90	61 994.92	QEA03466.1	99	86.64
DsylCXE39	589	是	7.13	65 697.92	WBO25685.1	72	80.84

基因名称	ORF长度/aa	是否有完整ORF	等电点pI	分子量/Da	最佳匹配登录号	覆盖率/%	相似度/%
DsylAOX1	133	否	4.94	15 161.26	QZC92247.1	100	97.01
DsylAOX2	1 028	是	5.33	115 397.04	NP_001299599.1	100	79.57
DsylAOX3	134	否	8.42	14 787.10	NP_001299599.1	96	91.47
DsylAOX4	1 280	是	6.39	142 514.41	BAR64767.1	100	72.76
DsylAOX5	589	是	9.97	66 171.90	QPF77600.1	68	63.75
DsylAOX6	372	否	5.33	42 054.92	XP_053608971.1	100	80.97

Study on Screening of Odorant Degradation Enzyme Genes in Dioryctria sylvestrella and its Expression Level in Different Tissues

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基因名称	ORF长度/aa	是否有完整ORF	等电点pI	分子量/Da	最佳匹配登录号	覆盖率/%	相似度/%
DsylGST1	97	否	4.49	11 307.85	WBO25703.1	100	92.86
DsylGST2	68	否	4.78	7 828.27	WBO25700.1	100	98.55
DsylGST3	65	否	5.72	7 661.77	WBO25700.1	100	96.97
DsylGST4	57	否	7.51	6 505.81	WBO25700.1	100	100.00
DsylGST5	101	是	9.16	11 257.77	XP_053614950.1	95	83.33
DsylGST6	244	是	9.12	28 188.68	XP_053614946.1	100	73.77
DsylGST7	68	否	6.95	7 760.02	WBO25698.1	100	100.00
DsylGST8	235	是	6.80	27 007.98	XP_060802045.1	99	87.98
DsylGST9	68	否	9.98	7 803.42	NP_001299595.1	100	60.87
DsylGST10	241	是	5.76	27 083.19	WBO25700.1	98	96.19
DsylGST11	289	是	7.23	33 226.65	WBO25693.1	100	89.27
DsylGST12	221	是	6.53	24 972.69	XP_053614426.1	97	85.12
DsylGST13	219	是	5.86	24 718.29	WBO25696.1	87	95.29
DsylGST14	246	是	5.32	27 579.84	NP_001299595.1	100	89.02
DsylGST15	216	是	4.93	24 438.09	UQS35834.1	100	78.60
DsylGST16	255	是	6.94	28 903.33	WBO25695.1	100	98.82
DsylGST17	225	是	9.18	25 376.48	WBO25699.1	91	93.66
DsylGST18	151	是	9.96	16 786.68	XP_053625608.1	100	86.93
DsylGST19	70	否	9.16	7 834.26	WBO25700.1	100	97.18
DsylGST20	204	是	6.30	23 546.20	WBO25701.1	100	94.61
DsylGST21	228	是	9.13	26 640.70	XP_013194289.1	100	82.46
DsylGST22	151	是	10.10	16 985.24	WBO25691.1	77	94.83
DsylGST23	214	是	7.69	24 064.50	WBO25698.1	100	97.20
DsylGST24	69	否	6.51	8 063.24	WBO25700.1	100	94.29
DsylGST25	74	是	5.03	8 782.15	WBO25693.1	100	81.08
DsylGST26	148	是	10.16	16 566.47	WBO25697.1	100	98.65
DsylGST27	299	是	7.04	26 110.28	XP_053611606.1	100	76.86
DsylGST28	40	否	10.96	4 455.30	NP_001299595.1	100	80.49
DsylGST29	113	否	5.07	12 937.99	WBO25700.1	94	72.48
DsylGST30	204	是	4.93	23 676.35	WBO25703.1	78	100.00
DsylGST31	248	是	8.75	28 661.32	WBO25692.1	86	99.53
DsylGST32	78	否	9.39	8 883.29	QCX41803.1	99	74.36

基因名称	ORF长度/aa	是否有完整ORF	等电点pI	分子量/Da	最佳匹配登录号	覆盖率/%	相似度/%
DsylCYP1	25	否	4.37	2 811.33	XP_060801165.1	100	69.23
DsylCYP2	260	否	5.26	30 196.61	QZP43566.1	100	77.01
DsylCYP3	203	否	7.40	23 604.40	XP_053613700.1	100	87.75
DsylCYP4	232	否	6.79	26 583.99	XP_064292090.1	100	80.60
DsylCYP5	79	否	3.87	9 071.09	ULR85505.1	100	86.25
DsylCYP6	61	否	10.48	7 354.68	XP_060805041.1	100	70.97
DsylCYP7	64	否	4.86	7 457.61	XP_013183008.2	100	84.62
DsylCYP8	68	否	4.36	7 856.90	WBO25717.1	100	86.96
DsylCYP9	120	否	4.20	14 186.11	WBO25708.1	98	94.12
DsylCYP10	109	否	6.09	12 554.26	GLH02031.1	100	100.00
DsylCYP11	43	否	4.24	4 887.45	QPF77601.1	100	95.45
DsylCYP12	90	否	7.96	10 338.05	XP_060800329.1	100	80.22
DsylCYP13	63	否	5.70	7 164.29	QZP43568.1	98	90.48
DsylCYP14	499	是	7.91	57 745.26	WBO25716.1	100	90.36
DsylCYP15	50	否	10.60	6 359.78	XP_013196728.2	98	82.00
DsylCYP16	143	是	10.51	16 007.83	QZP43575.1	100	88.11
DsylCYP17	383	否	6.34	45 061.09	XP_013183823.2	96	72.90
DsylCYP18	593	是	6.51	61 766.51	QZP43558.1	100	86.27
DsylCYP19	511	是	8.38	58 470.84	XP_013186352.1	100	74.46
DsylCYP20	531	是	8.06	60 900.52	QZP43566.1	100	72.69
DsylCYP21	481	是	6.34	56 412.28	NP_001351990.1	99	78.48
DsylCYP22	531	是	9.20	61 763.66	XP_013199514.1	100	86.23
DsylCYP23	505	是	9.03	57 711.08	XP_060804943.1	98	740.4
DsylCYP24	513	是	8.44	58 529.74	XP_013184491.1	100	82.26
DsylCYP25	502	是	9.05	58 062.36	XP_013183008.2	99	74.25
DsylCYP26	531	是	9.55	60 886.97	XP_053625564.1	98	64.15
DsylCYP27	197	否	9.51	23 097.04	WBO25718.1	100	94.44
DsylCYP28	513	是	9.35	58 743.97	QZP43553.1	100	75.88
DsylCYP29	498	是	9.06	57 031.47	XP_013192856.2	100	80.92
DsylCYP30	517	是	8.92	59 152.29	QZP43555.1	98	79.13
DsylCYP31	515	是	9.66	59 588.69	XP_013184001.2	99	81.45
DsylCYP32	502	是	9.25	58 599.23	XP_013183823.2	97	70.70
DsylCYP33	515	是	9.00	58 784.25	WBO25715.1	99	96.88
DsylCYP34	512	是	8.82	59 185.80	WBO25709.1	98	96.83
DsylCYP35	412	否	9.55	47 467.23	XP_013188296.2	99	54.99
DsylCYP36	510	是	9.25	57 968.50	XP_060801165.1	97	62.87
DsylCYP37	559	是	9.08	63 964.16	WBO25721.1	100	97.13
DsylCYP38	524	是	8.86	61 057.87	XP_060802800.1	99	78.08
DsylCYP39	684	是	5.22	76 991.49	WBO25714.1	100	96.35
DsylCYP40	439	是	9.74	50 541.80	XP_013188296.2	100	55.91
DsylCYP41	522	是	8.53	61 340.15	XP_060806632.1	99	78.61
DsylCYP42	493	是	8.67	57 387.32	XP_053601586.1	97	77.41
DsylCYP43	481	是	6.72	53 682.25	XP_013183200.1	100	86.07
DsylCYP44	540	是	7.52	61 379.28	QZP43565.1	100	95.37
DsylCYP45	87	是	10.19	10 410.61	XP_013185828.2	36	100.00
DsylCYP46	258	是	8.77	29 618.27	XP_059050485.1	100	54.82
DsylCYP47	515	是	8.71	59 383.15	XP_060803177.1	100	86.27
DsylCYP48	510	是	8.28	59 295.82	QZP43571.1	100	79.80
DsylCYP49	45	否	8.93	5 504.43	XP_013185828.2	100	93.48
DsylCYP50	103	是	11.06	12 188.29	QBA57446.1	96	74.75
DsylCYP51	235	是	7.86	27 033.33	QZP43554.1	95	72.65
DsylCYP52	509	是	9.71	57 937.54	XP_013192036.1	100	77.76
DsylCYP53	522	是	8.30	61 249.98	XP_060806632.1	99	78.61
DsylCYP54	48	是	9.97	5 665.76	XP_060804943.1	94	66.67
DsylCYP55	521	是	7.24	60 774.40	XP_053617327.1	100	76.15
DsylCYP56	169	是	10.10	19 784.42	XP_013188296.2	41	69.44
DsylCYP57	66	是	10.82	7 665.25	XP_013199514.1	98	92.31
DsylCYP58	166	是	6.11	18 856.61	XP_013189127.2	95	89.17
DsylCYP59	244	否	7.03	28 080.67	QBA57484.1	100	96.72
DsylCYP60	507	是	8.58	58 260.37	XP_053613686.1	100	69.57

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