Abstract:

Alternariol （AOH） is a toxic metabolite produced by the fungus Alternariol. By infecting wheat， tomato， cherry， citrus and other agricultural products， Streptomyces alternans not only causes economic losses but also endangers human health. In this study， based on the principle of competition， a sensitive and specific aptamer fluorescence test strip for AOH detection was constructed. After optimizing the aptamer concentration， pH value and buffer， the accuracy and specificity of the aptamer were further evaluated and applied to the determination of AOH in wheat samples. Under the optimal conditions， the detection limit was 1 μg·L^-1， the detection range was 1~1 000 μg·L^-1， the linear correlation coefficient R²=0.979 2， the detection could be completed in only 20 minutes， the recovery rate in the actual sample analysis was 96.6%~114.3%， the relative standard deviation was 2.6%~9.0%. In this study， a simple， rapid and accurate new method for rapid quantitative detection of AOH was developed using aptamer as a recognition element， which would provide a reference for the development of other mycotoxin rapid detection technologies.

Key words: aptamer, fluorescent lateral flow test strips, alternariol, wheat

摘要：

交链孢酚（alternariol，AOH），作为交链孢霉菌产生的一种有毒代谢产物，通过侵染小麦、番茄、樱桃和柑橘等农产品，不仅造成经济损失，还对人类健康构成威胁。基于竞争原理，构建了一种针对AOH的灵敏且特异性强的核酸适配体荧光试纸条检测体系。对适配体浓度、pH和缓冲液等条件进行优化后，进一步评价其准确性和特异性等性能，并应用于小麦样品中AOH的含量测定。在最优条件下，其检测限可达1 μg·L^-1，检测范围为1~1 000 μg·L^-1，线性相关系数R²=0.979 2，仅需20 min即可完成检测。在实际样品分析中的回收率为96.6%~114.3%，相对标准偏差为2.6%~9.0%。利用核酸适配体作为识别元件，开发了一种简便、快速、准确的适合现场快速定量检测AOH的新方法，为其他真菌毒素快速检测技术的研发提供了参考。

关键词: 核酸适配体, 荧光侧流层析试纸条, 交链孢酚, 小麦