Live Microbial Agriculture：Reconstructing the Application Model of Agricultural Microorganisms and New Driving Forces for Industrial Development

doi:10.19586/j.2095-2341.2025.0095

Current Biotechnology ›› 2025, Vol. 15 ›› Issue (5): 747-754.DOI: 10.19586/j.2095-2341.2025.0095

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Live Microbial Agriculture：Reconstructing the Application Model of Agricultural Microorganisms and New Driving Forces for Industrial Development

Xiaofeng GAO¹(), Wenjing CUI²(), Jinfeng WEI¹, Zhu LIU³, Min LIN¹(), Ruiqiang MA²()

^1.Food Laboratory of Zhongyuan，College of Agriculture，Henan University，Henan Kaifeng 475004，China
^2.Beijing Green Nitrogen Biotech Co. ，Ltd. ，Beijing 100094，China
^3.Faculty of Animal Science and Technology，Yunnan Agricultural University，Kunming 650201，China

Received:2025-07-30 Accepted:2025-10-23 Online:2025-09-25 Published:2025-11-11
Contact: Min LIN,Ruiqiang MA

Abstract

Abstract:

Live microbial agriculture is an innovative agricultural model that uses on-site intelligent fermentation as its core technology. Through beneficial microbial on-site intelligent fermentation technology， highly active microorganisms are directly cultivated in application scenarios such as field planting， pond mouth breeding， and facility agriculture. Liquid live microbial preparations are used for immediate and nearby application， breaking the bottleneck problems of long production cycles， declining field vitality， and low application effects in traditional agricultural microbial technology. At present， research on live microbial agricultural microbiology technology is still in its initial stage and there have been no relevant reports. This review summarized the multidimensional advantages， intelligent fermentation technology， main functions， and application areas of live microbial agriculture. It also looks forward to the future application prospects of live microbial agriculture， aiming to provide new impetus for improving agricultural production efficiency， reshaping the application mode and industrial development of agricultural microorganisms.

Key words: live microbial agriculture, on-site intelligent fermentation, highly active bacterial agent, application model innovation

摘要：

活菌农业是一种以现场智能发酵为核心技术的创新农业模式，通过有益微生物现场智能发酵技术，在田间种植、塘口养殖和设施农业等应用场景中直接培育高活性微生物，以液态活菌制剂形式实现现做现用和就近施用，有望打破传统农业微生物技术存在的生产周期长、田间活力衰退和应用效果低下等瓶颈问题。目前，活菌农业微生物技术研究还处于初始阶段，尚未有相关报道。综述梳理了活菌农业的多维度优势、智能发酵技术及其主要功能和应用领域，并对活菌农业的未来应用前景进行了展望，旨在为提升农业生产效率和重塑农业微生物应用模式与产业发展提供新动力。

关键词: 活菌农业, 现场智能发酵, 高活力菌剂, 应用模式创新

CLC Number:

Q939.9

Xiaofeng GAO, Wenjing CUI, Jinfeng WEI, Zhu LIU, Min LIN, Ruiqiang MA. Live Microbial Agriculture：Reconstructing the Application Model of Agricultural Microorganisms and New Driving Forces for Industrial Development[J]. Current Biotechnology, 2025, 15(5): 747-754.

郜晓峰, 崔文靖, 魏金凤, 刘柱, 林敏, 马瑞强. 活菌农业：重塑农业微生物应用模式与产业发展新动能[J]. 生物技术进展, 2025, 15(5): 747-754.

Figures/Tables 5

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类型	传统菌剂发酵	智能活菌发酵	智能活菌优势
设备投资	50~100万	20~60万	投资低40% 投产快80%
场地建设	需要	无需特殊场地
安装调试	3个月以上	无需现场安装
生产周期	3~5 d·次^-1	1 d·次^-1	效率高50%
发酵效率	200亿	100亿 (3 d累计300亿)	效率高50%
运营人员	专业发酵工程师	无需发酵工程师	人力省80%

类型	传统菌剂发酵	智能活菌发酵	智能活菌优势
设备投资	50~100万	20~60万	投资低40% 投产快80%
场地建设	需要	无需特殊场地
安装调试	3个月以上	无需现场安装
生产周期	3~5 d·次^-1	1 d·次^-1	效率高50%
发酵效率	200亿	100亿 (3 d累计300亿)	效率高50%
运营人员	专业发酵工程师	无需发酵工程师	人力省80%

应用领域	生产菌种	智能培菌机活菌数/(CFU·mL^-1)	智能活菌站活菌数/(CFU·mL^-1)	主要功能
植保领域	苏云金芽孢杆菌	≥10亿	≥20亿	防治棉铃虫、玉米螟、菜青虫、马铃薯甲虫、金龟子和松毛虫等
	哈茨木霉	≥2亿	≥5亿	防治病害：立枯病、猝倒病、根腐病、灰霉病、白粉病等；促进生长：根系发育、抗逆性增强
	淡紫拟青霉	≥2亿	≥5亿	防治线虫：根结线虫、胞囊线虫等；防治害虫：蚜虫、粉虱、蓟马等；防治土传病害：立枯病、猝倒病等
	根瘤菌	≥2亿	≥5亿	豆科作物生物固氮，促进结瘤
	白僵菌	≥2亿	≥5亿	防治棉铃虫、玉米螟、稻纵卷叶螟、马铃薯甲虫、金龟子、蚜虫、粉虱、松毛虫、天牛等
	复合芽孢杆菌	≥50亿	≥100亿	土壤改良、病害防治、促进植物生长、改善作物品质
水产领域	丁酸梭菌	≥5亿	≥10亿	促进消化吸收、改善肠道微生态、增强免疫力、促进生长
	乳酸菌	≥50亿	≥100亿	分解水中的有机废弃物，改善水质，减少氨氮和亚硝酸盐的积累，通过维持水体微生态平衡，减少病原菌的滋生
	芽孢杆菌	≥50亿	≥100亿	降解有机废弃物、改善水体微生态、稳定水体环境、促进藻类平衡、增加溶氧
畜牧领域	禽类专用：丁酸梭菌+凝结芽孢杆菌	≥10亿	≥10亿	促进消化吸收、改善肠道微生态、增强免疫力、促进生长
畜牧领域	猪和反刍动物专用：乳酸菌	≥20亿	≥100亿	促进消化吸收、改善肠道微生态、增强免疫力、促进生长
环保领域	复合乳酸菌、复合芽孢杆菌	≥50亿	≥100亿	除臭：源头分解臭气分子，去除氨气、硫化氢等臭源，降低臭味浓度
环保领域	复合芽孢杆菌	≥50亿	≥100亿	堆肥：快速升温，维持高温，腐熟充分

应用领域	生产菌种	智能培菌机活菌数/(CFU·mL^-1)	智能活菌站活菌数/(CFU·mL^-1)	主要功能
植保领域	苏云金芽孢杆菌	≥10亿	≥20亿	防治棉铃虫、玉米螟、菜青虫、马铃薯甲虫、金龟子和松毛虫等
	哈茨木霉	≥2亿	≥5亿	防治病害：立枯病、猝倒病、根腐病、灰霉病、白粉病等；促进生长：根系发育、抗逆性增强
	淡紫拟青霉	≥2亿	≥5亿	防治线虫：根结线虫、胞囊线虫等；防治害虫：蚜虫、粉虱、蓟马等；防治土传病害：立枯病、猝倒病等
	根瘤菌	≥2亿	≥5亿	豆科作物生物固氮，促进结瘤
	白僵菌	≥2亿	≥5亿	防治棉铃虫、玉米螟、稻纵卷叶螟、马铃薯甲虫、金龟子、蚜虫、粉虱、松毛虫、天牛等
	复合芽孢杆菌	≥50亿	≥100亿	土壤改良、病害防治、促进植物生长、改善作物品质
水产领域	丁酸梭菌	≥5亿	≥10亿	促进消化吸收、改善肠道微生态、增强免疫力、促进生长
	乳酸菌	≥50亿	≥100亿	分解水中的有机废弃物，改善水质，减少氨氮和亚硝酸盐的积累，通过维持水体微生态平衡，减少病原菌的滋生
	芽孢杆菌	≥50亿	≥100亿	降解有机废弃物、改善水体微生态、稳定水体环境、促进藻类平衡、增加溶氧
畜牧领域	禽类专用：丁酸梭菌+凝结芽孢杆菌	≥10亿	≥10亿	促进消化吸收、改善肠道微生态、增强免疫力、促进生长
畜牧领域	猪和反刍动物专用：乳酸菌	≥20亿	≥100亿	促进消化吸收、改善肠道微生态、增强免疫力、促进生长
环保领域	复合乳酸菌、复合芽孢杆菌	≥50亿	≥100亿	除臭：源头分解臭气分子，去除氨气、硫化氢等臭源，降低臭味浓度
环保领域	复合芽孢杆菌	≥50亿	≥100亿	堆肥：快速升温，维持高温，腐熟充分

Live Microbial Agriculture：Reconstructing the Application Model of Agricultural Microorganisms and New Driving Forces for Industrial Development

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