Abstract:

Antibodies have attracted significant attention in disease treatment， especially in cancer therapy， owing to their high target selectivity. However， traditional antibody discovery methods， such as hybridoma technology， are complex to operate and dependent on animal models， rendering them increasingly inadequate to meet the needs of modern large-scale antibody screening. Consequently， there is an urgent need to develop novel high-throughput antibody screening approaches. Fab synthetic phage libraries offer advantages including lower cost， higher efficiency， and the capability to generate custom-specific antibodies， which is conducive to the identification of new disease targets and large-scale antibody screening. This review systematically summarized the research progress and clinical applications of Fab synthetic phage display libraries in terms of library design， display systems， and screening methods， with the aim of providing references for optimizing antibody screening and engineering.

Key words: Fab antibody, phage display library, synthetic library, antibody discovery

摘要：

抗体因其靶向性强在疾病治疗中尤其是癌症治疗中备受关注。然而，由于操作复杂且依赖动物免疫，杂交瘤技术等传统抗体发现手段难以满足现代大规模筛选的需求。因此，迫切需要开发新的高通量抗体筛选方法。Fab合成噬菌体文库用于抗体筛选的成本更低、效率更高，且能定制特异性抗体，这有利于疾病新靶点的发现和抗体的大规模筛选。系统综述了Fab合成噬菌体文库在文库设计、展示系统、筛选方法等方面的研究进展以及临床应用，以期对抗体筛选和工程的优化提供参考。

关键词: Fab抗体, 噬菌体文库, 合成文库, 抗体发现