Research Progress of Riboswitch Biosensors for Small Molecule Target

doi:10.19586/j.2095-2341.2021.0140

Current Biotechnology ›› 2022, Vol. 12 ›› Issue (2): 168-175.DOI: 10.19586/j.2095-2341.2021.0140

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Research Progress of Riboswitch Biosensors for Small Molecule Target

Yifan WU¹(), Shenghao LIN², Wentao XU¹^,²()

^1.College of Food Science and Nutritional Engineering，China Agricultural University，Beijing 100083，China
^2.Key Laboratory of Precision Nutrition and Food Quality，Department of Nutrition and Health，China Agricultural University，Beijing 100083，China

Received:2021-08-05 Accepted:2021-09-03 Online:2022-03-25 Published:2022-03-25
Contact: Wentao XU

小分子靶标的核糖开关生物传感器研究进展

吴一凡¹(), 林晟豪², 许文涛¹^,²()

^1.中国农业大学食品科学与营养工程学院，北京 100083
^2.中国农业大学营养与健康系，食品精准营养与质量控制教育部重点实验室，北京 100083

通讯作者: 许文涛
作者简介:吴一凡 E-mail: Wyf001208@126.com;
基金资助:
山东省重大科技创新工程项目(2019JZZY011014);北京市农业科技项目(PXM2021_014207_000037)

Abstract

Abstract:

There are many kinds of small molecule compounds， and play key roles in many biological processes， which has important detection significance and value. The development of its rapid and sensitive visual detection technology is a research hotspot at present. Biosensor based on riboswitch provides a new way for small molecule detection due to its advantages， such as high recognition characteristics， simple operation and low cost. In this paper， the source， composition， regulatory mechanism， in vivo and in vitro screening of riboswitches， especially the classification of riboswitch biosensors based on small molecular targets were introduced， which aimed to provide a theoretical basis for the development and application of small molecule target riboswitch biosensor.

Key words: small molecule target, riboswitch, biosensor, screen, gene expression regulation

摘要：

小分子化合物种类繁多，在众多生化过程中发挥关键作用，具有重要的检测意义与价值,其快速灵敏可视化检测技术的开发是当前的研究热点。基于核糖开关的生物传感器因具有识别特性高、操作简便、成本低等优势，为小分子检测提供了一条新途径。对核糖开关的来源、构成、调控机制、体内外筛选，特别是对基于小分子靶标的核糖开关生物传感器分类进行了介绍，并从核糖开关的筛选、裁剪、理性设计、核糖开关无细胞传感器的应用等几个方向提出了展望，以期为小分子靶标的核糖开关生物传感器的发展和应用提供理论依据。

关键词: 小分子靶标, 核糖开关, 生物传感器, 筛选, 基因表达调控

CLC Number:

Q522

Yifan WU, Shenghao LIN, Wentao XU. Research Progress of Riboswitch Biosensors for Small Molecule Target[J]. Current Biotechnology, 2022, 12(2): 168-175.

吴一凡, 林晟豪, 许文涛. 小分子靶标的核糖开关生物传感器研究进展[J]. 生物技术进展, 2022, 12(2): 168-175.

Figures/Tables 2

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传感器类别	优点	缺点
核糖开关传感器	应用范围广，识别元件可快速响应，高特异性和灵敏度，成本低	结构刚性不足，人工合成筛选
酶生物传感器	制备简便，高特异性	应用范围相对较窄，成本高，易失活，稳定性较低
免疫传感器	抗原抗体识别特异性好	应用范围相对较窄，成本高，稳定性较低
微生物传感器	成本低，耐久性好	稳定性、灵敏度低，响应时间久
细胞传感器	成本低，制作简便	应用范围有限，稳定性较低

传感器类别	优点	缺点
核糖开关传感器	应用范围广，识别元件可快速响应，高特异性和灵敏度，成本低	结构刚性不足，人工合成筛选
酶生物传感器	制备简便，高特异性	应用范围相对较窄，成本高，易失活，稳定性较低
免疫传感器	抗原抗体识别特异性好	应用范围相对较窄，成本高，稳定性较低
微生物传感器	成本低，耐久性好	稳定性、灵敏度低，响应时间久
细胞传感器	成本低，制作简便	应用范围有限，稳定性较低

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Research Progress of Riboswitch Biosensors for Small Molecule Target

小分子靶标的核糖开关生物传感器研究进展

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