Abstract: With the increasing demand for mango from Yunnan, the planting area of mango has been expanding, and new varieties are continuously introduced, thus causing the occurrence of anthracnose disease of mango in Yunnan Province to become more and more serious. In order to carry out targeted biological control of mango anthracnose, the pathogen of postharvest mango anthracnose was isolated by tissue block separation method. Morphological observation was used as preliminary identification, and the pathogen was verified according to Koch’s postulates. Subsequently, rDNA-ITS sequence and phylogenetic tree analysis were used to identify the taxonomic status of pathogenic bacteria. Finally, 5 kinds of biocontrol bacteria were used to conduct antagonistic tests on pathogenic bacteria. Through isolation and identification of the pathogen causing mango postharvest anthracnose, Colletotrichum gloeosporioides was determined to be the pathogen causing mango postharvest anthracnose in Yunnan Province. The sequence length of internal transcribed spacer (ITS) in this strain was 536 bp and the login number was MH744668. The five biocontrol bacteria had certain bacteriostatic to mango anthracnose pathogenic bacteria, and had good biocontrol development potential. Among them, the antibiotic L-44 had the best bacteriostatic effect, with the inhibition rate of 53.7%. The research results provided a new insight into biological control of mango postharvest diseases in Yunnan Province.

Key words: mango anthracnose disease, Colletotrichum gloeosporioide, Koch’s postulates, biocontrol bacterium screening

摘要： 随着对云南芒果需求量的增加，其种植面积日趋扩大，并不断引入新品种，这也导致云南省芒果炭疽病害发生日趋加重。为了有针对性地开展芒果炭疽病的生物防治，采用组织块分离法分离芒果采后炭疽病病原菌，通过形态学观察初步鉴定，并遵循柯赫氏法则对病原菌进行验证。随后，利用rDNA-ITS序列和系统发育树分析明确病原菌的分类学地位。最后，采用5种生防细菌对病原菌进行拮抗试验。通过对芒果采后炭疽病病原菌进行分离鉴定，确定胶孢炭疽菌（Colletotrichum gloeosporioides）是引起云南芒果采后炭疽病的病原菌，该菌株内转录间隔区（internal transcribed spacer，ITS）序列长度为536 bp，登录号为MH744668；5株生防细菌对芒果炭疽病病原菌都有一定的抑菌作用，具有较好的生防开发潜能，其中抗生素溶杆菌L-44的抑菌效果最好，抑制率达53.7%。研究结果为云南省芒果采后病害的生物防治提供了新的思路。

关键词: 芒果炭疽病, 胶孢炭疽菌, 柯赫氏法则, 生防细菌筛选