大豆GmMYB109转录因子的亚细胞定位及表达模式分析

doi:10.19586/j.2095-2341.2026.0057

生物技术进展 ›› 2026, Vol. 16 ›› Issue (4): 919-925.DOI: 10.19586/j.2095-2341.2026.0057

大豆GmMYB109转录因子的亚细胞定位及表达模式分析

张鸿棋¹(), 于月华²(), 倪志勇¹()

^1.新疆农业大学生命科学学院，新疆极端环境生物生态适应与进化自治区重点实验室，乌鲁木齐 830052
^2.新疆农业大学农学院，乌鲁木齐 830052

收稿日期:2026-03-06 接受日期:2026-04-15 出版日期:2026-07-25 发布日期:2026-09-11
通信作者: 于月华,倪志勇
作者简介:张鸿棋 E-mail: 756377698@qq.com
基金资助:
“天山英才培养”计划项目(2023TSYCCX0012);新疆维吾尔自治区自然科学基金项目(2025D01E14);国家自然科学基金项目(32572298)

Analysis of Subcellular Localization and Expression Patterns of Soybean Transcription Factor GmMYB109

Hongqi ZHANG¹(), Yuehua YU²(), Zhiyong NI¹()

^1.Xinjiang Key Laboratory for Ecological Adaptation and Evolution of Extreme Environment Organisms，College of Life Sciences，Xinjiang Agricultural University，Urumqi 830052，China
^2.College of Agronomy，Xinjiang Agricultural University，Urumqi 830052，China

Received:2026-03-06 Accepted:2026-04-15 Online:2026-07-25 Published:2026-09-11
Contact: Yuehua YU,Zhiyong NI

摘要/Abstract

摘要：

为了探究大豆中GmMYB109转录因子的功能，利用生物信息学方法对其序列进行分析，烟草瞬时表达分析其亚细胞定位，qRT-PCR测定其在不同组织以及盐胁迫不同时段下的表达量。生物信息学预测结果表明GmMYB109有2个SANT结构域且定位于细胞核中；瞬时表达结果表明其定位于烟草表皮的细胞核中，说明GmMYB109是一个核定位的转录因子；qRT-PCR结果表明GmMYB109在叶中表达量最高，其次为茎、子叶和根，且在盐胁迫下GmMYB109表达量逐渐升高，表明其受盐胁迫诱导表达。研究结果可为进一步探讨GmMYB109转录因子的生物学功能和作用机理提供理论依据。

关键词: 大豆, MYB转录因子, 亚细胞定位, 盐胁迫

Abstract:

To investigate the function of the GmMYB109 transcription factor in soybean， we performed bioinformatics analysis on its sequence， examined its subcellular localization using transient expression in tobacco， and determined its expression levels in different tissues and at different time points under salt stress by qRT?PCR. Bioinformatics prediction showed that GmMYB109 contains two SANT domains and is localized in the nucleus. Transient expression assays confirmed its nuclear localization in tobacco epidermal cells， indicating that GmMYB109 is a nuclear?localized transcription factor. qRT?PCR results revealed that GmMYB109 was most highly expressed in leaves， followed by stems， cotyledons and roots. Its expression gradually increased under salt stress， suggesting that GmMYB109 is induced by salt stress. These results provide theoretical basis for further exploring the biological function and regulatory mechanism of the GmMYB109 transcription factor.

Key words: soybean, MYB transcription factor, subcellular localization, salt stress

中图分类号:

Q291

张鸿棋, 于月华, 倪志勇. 大豆GmMYB109转录因子的亚细胞定位及表达模式分析[J]. 生物技术进展, 2026, 16(4): 919-925.

Hongqi ZHANG, Yuehua YU, Zhiyong NI. Analysis of Subcellular Localization and Expression Patterns of Soybean Transcription Factor GmMYB109[J]. Current Biotechnology, 2026, 16(4): 919-925.

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链接本文: https://swjsjz.magtechjournal.com/CN/10.19586/j.2095-2341.2026.0057

https://swjsjz.magtechjournal.com/CN/Y2026/V16/I4/919

图/表 5

表1 本研究使用的引物序列

Table 1 Primer sequences used in this study

引物名称	引物序列（5'→3'）	用途
GmMYB109-F	5'-ATGTCATCAAGTGGAACCATTTGGAGCTAT-3'	基因克隆
GmMYB109-R	5'-5'-TTATTGATGCATTGTTGGAGGTGCCAT-3'	基因克隆
GmMYB109-pCAMBIA1304-F	5'-cgggggactcttgaccatggATGTCATCAAGTGGAACCATTTGGAGCTAT-3'	亚细胞定位
GmMYB109-pCAMBIA1304-R	5'-ctcctttactagtcagatctTTGATGCATTGTTGGAGGTGCCAT-3'	亚细胞定位
GmMYB109-qPCR-F	5'-AGTTGGACATCCTGTGGCTG-3'	荧光定量
GmMYB109-qPCR-R	5'-GATGAGGATGAGCATGGGGG-3'	荧光定量
GmCYP2-F	5'-CGGGACCAGTGTGCTTCTTCA-3'	荧光定量内参引物
GmCYP2-R	5'-CCCCTCCACTACAAAGGCTCG-3'	荧光定量内参引物

图1 GmMYB109的克隆及pCAMBIA1304载体构建A：GmMYB109的克隆，泳道3—目的条带，泳道1、2、4—阴性对照；B：GmMYB109-pCAMBIA1304-DH5α载体构建，M—2000 DNA marker；1~4—实验样品；C：GmMYB109-pCAMBIA1304-GV3101转化，M—2000 DNA marker；1~4—实验样品。

Fig. 1 Cloning of GmMYB109 and construction of pCAMBIA1304 vector

图2 GmMYB109的生物信息学分析A：GmMYB109的进化树分析，Glycine max—大豆，Vigna angularis—赤豆，Mucuna pruriens—刺毛黧豆，Gastrolobium bilobum—毒羊豆，Lotus japonicus—百脉根，Medicago truncatula—蒺藜苜蓿，Cicer arietinum—鹰嘴豆，Lupinus albus—白羽扇豆，Stylosanthes scabra—西卡柱花草，Bauhinia variegata—宫粉羊蹄甲，Prosopis cineraria—灰牧豆树，Senna tora—决明，Quillaja saponaria—皂皮树，Fagus crenata—齿水青冈，Juglans regia—胡桃，Morella rubra—杨梅；B：GmMYB109蛋白的结构域分析；C：GmMYB109蛋白的二级结构预测；D：GmMYB109蛋白的疏水性分析

Fig. 2 Bioinformatics analysis of GmMYB109

图3 GmMYB109的亚细胞定位结果

Fig. 3 Subcellular localization results of GmMYB109

图4 GmMYB109基因的表达模式分析A：GmMYB109基因的不同组织表达模式分析；B：250 mmol·L-1 NaCl胁迫下GmMYB109基因在不同时段表达模式分析；*、****分别表示差异在P<0.05、P<0.0001水平上具有统计学意义。

Fig. 4 Expression pattern analysis of the GmMYB109 gene

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大豆GmMYB109转录因子的亚细胞定位及表达模式分析

Analysis of Subcellular Localization and Expression Patterns of Soybean Transcription Factor GmMYB109

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