Isolation and Identification of a Salt-tolerance Myxobacteria

doi:10.3969/j.issn.2095-2341.2013.04.11

Curr. Biotech. ›› 2013, Vol. 3 ›› Issue (4): 288-292.DOI: 10.3969/j.issn.2095-2341.2013.04.11

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Isolation and Identification of a Salt-tolerance Myxobacteria

ZHANG Xian-jiao, XIE Xiao-lin, CHEN Mei-biao, DUAN Jin-mei, LI Yan-xuan, ZHU Hong-hui

Guangdong Institute of Microbiology, Gangzhou Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, State Key Laboratory of Applied Microbiology, South China (MinistryProvince Jointly Development), Guangzhou 510070, China

Received:2013-05-29 Online:2013-07-25 Published:2013-07-03

一株耐盐粘细菌的分离与鉴定

张鲜姣,谢小林,陈美标,段锦梅,李燕璇,朱红惠*

广东省微生物研究所, 广东省菌种保藏与应用重点实验室, 广东省微生物应用新技术公共实验室, 省部共建华南应用微生物国家重点实验室, 广州 510070

通讯作者: 朱红惠,研究员，主要从事微生物资源环境方向研究。E-mai:zhuhonghui66@yahoo.com.cn
作者简介:张鲜姣，硕士，研究方向为粘细菌的分离鉴定。E-mail:zhangxianjiao2008@163.com。
基金资助:
国家自然科学基金项目（31170009），广东省自然科学基金项目（10151007002000008），新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室开放基金课题重点项目（BRZD1004）资助。

Abstract

Abstract: Base on conventional isolational method, a salttolerance myxobacteria was isolated from the saline-alkaline soils of Akesu in Xinjiang. According to the morphologies of swarms, fruiting bodies,vegetative cells, myxospores, physiological and biochemical characteristic and the sequence of 16S rDNA, the strain was identified to Corallococcus macrosporus. Analysis of salt-tolerance showed the strain 5x1 could survive in NaCl concentration of 2%.

Key words: salt-tolerance, Corallococcus macrosporus, identification

摘要： 采用传统的子实体转接法和反复纯化法，从新疆阿克苏地区盐碱地土壤样品中分离1株粘细菌，经过对其菌落、子实体、营养细胞、粘孢子大小形态观察、生理生化特征试验及16S rDNA序列分析，鉴定菌株5x1为大孢珊瑚球菌（Corallococcus macrosporus），在对其进行耐盐性分析时发现，菌株5x1能耐受NaCl的浓度为2%。结果表明：菌株5x1为陆生耐盐粘细菌。

关键词: 耐盐, 大孢珊瑚球菌, 鉴定

ZHANG Xian-jiao, XIE Xiao-lin, CHEN Mei-biao, DUAN Jin-mei, LI Yan-xuan, ZHU Hong-hui*. Isolation and Identification of a Salt-tolerance Myxobacteria[J]. Curr. Biotech., 2013, 3(4): 288-292.

张鲜姣,谢小林,陈美标,段锦梅,李燕璇,朱红惠. 一株耐盐粘细菌的分离与鉴定[J]. 生物技术进展, 2013, 3(4): 288-292.

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Isolation and Identification of a Salt-tolerance Myxobacteria

一株耐盐粘细菌的分离与鉴定

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