Genetic Relationship of 40 Maize Inbred Lines Based on ISSR

doi:10.3969/j.issn.2095-2341.2011.03.11

journal1 ›› 2011, Vol. 1 ›› Issue (3): 214-218.DOI: 10.3969/j.issn.2095-2341.2011.03.11

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Genetic Relationship of 40 Maize Inbred Lines Based on ISSR

WANG Han-yu, Du Yan-wei, LI Ping, ZHANG Xi-wen, ZHU Jing-ying, ZHAO Jin-feng, YU Ai-li

1.College of Life Sciences, Hebei Agricultural University, Hebei Baoding 071000, China;
2.Millet Research Institute, Shanxi Academy of Agricultural Sciences, Shanxi Changzhi 046011, China

Received:2011-06-23 Online:2011-09-25 Published:2011-07-29

40份玉米自交系的ISSR遗传多样性分析

王寒玉1,杜艳伟2,李萍2,张喜文2,朱晶莹1,赵晋锋2,余爱丽1*

1.河北农业大学生命科学学院, 河北保定 071000;
2.山西省农业科学院谷子研究所, 山西长治 046011

通讯作者: 余爱丽,副教授,主要从事植物基因工程研究工作。E-mail:yuailimail@126.com
作者简介:王寒玉,硕士研究生,主要从事分子生物学与生物化学研究工作。E-mail:wanghanyu125@126.com。
基金资助:
山西省自然科学基金项目(2011011028-2);国家谷糜产业技术体系项目资助。

Abstract

Abstract: Genetic relationship of 40 maize inbred lines were analyzed by inter-simple sequence repeat (ISSR) marker. Ten primers, which selected from 59 primers, were used to amplify the maize simple, 90 bands were amplified, including 81 polymorphic bands. The percentage of polymorphic bands was 90.00%. The results showed that plenty diversities existed in the maize inbred lines. Cluster analysis by UPGMA indicated that genetic similarity was from 0.65~1.00. The result revealed the genetic relationship between different maise inbred lines. All the result provide reference for to fine varieties improvement and breeding on molecular level.

Key words: maize, ISSR, genetic relationship, genetic diversity

摘要： 利用ISSR分子标记技术对40份玉米自交系进行亲缘关系分析。从59条ISSR引物中筛选出10条重复性高、多态性好的引物,分别对全部供试材料进行扩增,共扩出90条清晰谱带,其中多态性条带81条,多态性比率为90.00%,表明供试材料基因组DNA的多态性较高。用 NTSYSpc-2.10软件中的 UPGMA进行聚类分析,40份玉米自交系的遗传相似系数变化范围在0.65~1.00之间,在此基础上构建聚类分析树状图,揭示了玉米各自交系间的亲缘关系。本研究为今后分子水平上玉米优良自交系的改良与选育提供了一定的参考依据。

关键词: 玉米, ISSR分子标记, 亲缘关系, 遗传多样性

WANG Han-yu1, Du Yan-wei2, LI Ping2, ZHANG Xi-wen2, ZHU Jing-ying1, ZHAO Jin-feng2, YU Ai-li1*. Genetic Relationship of 40 Maize Inbred Lines Based on ISSR[J]. journal1, 2011, 1(3): 214-218.

王寒玉,杜艳伟,李萍,张喜文,朱晶莹,赵晋锋,余爱丽. 40份玉米自交系的ISSR遗传多样性分析[J]. 生物技术进展, 2011, 1(3): 214-218.

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Genetic Relationship of 40 Maize Inbred Lines Based on ISSR

40份玉米自交系的ISSR遗传多样性分析

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