关键词: 新型冠状病毒, 数字PCR, 核酸检测, &ldquo, 两步法&rdquo, 检测

Abstract: In 2020, corona virus disease 2019 (COVID-19) pandemic caused by SARS-CoV-2 outbreaks in many countries around the world, which poses a great challenge to the prevention and control of the epidemic. Nucleic acid detection by real-time fluorescent PCR(RT-PCR) is the main detection method used at this stage, but the situation of “false negative results”, multiple sampling and repeated detection is also frequent. There is an urgent need to establish a detection technology with higher sensitivity, less sample need and simple operation to screen out patients infected SARS-CoV-2. Digital PCR has shown promise as a precision nucleic acid quantification technology because of its high sensitivity and specificity, which has been widely used in the field of virus detection. In this paper, the structure of SARS-CoV-2 and the problems of detecting methods by RT-PCR were introduced. And the application prospects of digital PCR in the detection of the SARS-CoV-2 were described, which was expected to provide references for the development of more efficient detection kits.

Key words: SARS-CoV-2, digital PCR, nucleic acid detection, detecting in two steps