火龙果对果蝇肠道损伤的缓解作用研究

doi:10.19586/j.2095-2341.2025.0144

生物技术进展 ›› 2026, Vol. 16 ›› Issue (2): 372-379.DOI: 10.19586/j.2095-2341.2025.0144

• 研究论文 • 上一篇

火龙果对果蝇肠道损伤的缓解作用研究

韩菊¹(), 陈嘉鹏², 周琪¹, 陈红蕊¹, 吴大畅¹()

^1.大连医科大学基础医学院，辽宁大连 116044
^2.大连医科大学附属第一医院，辽宁大连 116000

收稿日期:2025-10-20 接受日期:2025-12-05 出版日期:2026-03-25 发布日期:2026-04-27
通讯作者: 吴大畅
作者简介:韩菊 E-mail: 19808603823@163.com；

Investigation on the Alleviative Effects of Hylocereus undatus on Intestinal Damage in Drosophila melanogaster

Ju HAN¹(), Jiapeng CHEN², Qi ZHOU¹, Hongrui CHEN¹, Dachang WU¹()

^1.College of Basic Medical Sciences，Dalian Medical University，Liaoning Dalian 116044，China
^2.The First Affiliated Hospital of Dalian Medical University，Liaoning Dalian 116000，China

Received:2025-10-20 Accepted:2025-12-05 Online:2026-03-25 Published:2026-04-27
Contact: Dachang WU

摘要/Abstract

摘要：

探究火龙果（Hylocereus undatus）对果蝇肠道损伤的缓解作用及可能的作用机制，以期为火龙果的开发和应用提供理论依据。利用葡聚糖硫酸钠（dextran sulfate sodium salt，DSS）诱导野生型黑腹果蝇（Drosophila melanogaster）急性肠道损伤模型，分析喂食正常培养基与含不同浓度（0.5%、1%、5%）火龙果的培养基对果蝇生存指标（爬行能力、活跃度和生存率）、抗氧化指标[超氧化物歧化酶（superoxide dismutase，SOD）、过氧化氢酶（catalase，CAT）、还原型谷胱甘肽（glutathione,GSH）、丙二醛]和肠道损伤情况（蓝精灵实验、乳酸脱氢酶（lactate dehydrogenase，D-LDH）、Ca²⁺含量）的影响。结果表明，通过DSS成功构建了果蝇急性肠道损伤模型；喂食含火龙果的培养基后，果蝇的生存率、爬行能力和活跃度有明显改善，超氧化物歧化酶活性、过氧化氢酶活性、乳酸脱氢酶活性和还原型谷胱甘肽含量上升，Ca²⁺和丙二醛含量下降，肠道损伤得到明显缓解，且高浓度组（含5%火龙果）作用效果最为显著。火龙果对DSS诱导的果蝇肠道损伤具有一定的缓解作用，这可能与火龙果作用于肠道细胞的损伤修复，增强抗氧化能力有关。

关键词: 火龙果, 黑腹果蝇, 肠道损伤, 抗氧化

Abstract:

The study aims to investigate the alleviating effect of Hylocereus undatus on the intestinal damage in fruit flies， and explore the possible mechanism， with the goal of providing theoretical basis for the development and application of H. undatus. An acute intestinal injury model of wild-type Drosophila melanogaster was induced by dextran sulfate sodium salt （DSS）. The effects of feeding normal medium and medium containing different concentrations （0.5%， 1%， 5%） of H. undatus on the survival indicators （locomotor ability， activity and survival rate）， antioxidant indicators （superoxide dismutase， catalase， reduced glutathione， malondialdehyde）， and intestinal injury conditions （smurf assay， lactate dehydrogenase， Ca²⁺ content determination） of Drosophila melanogaster were analyzed. The results showed that the acute intestinal injury model of Drosophila melanogaster was successfully constructed by DSS. After feeding with medium containing H. undatus， the survival rate， locomotor ability and activity of Drosophila melanogaster were significantly improved， the activities of superoxide dismutase， catalase and lactate dehydrogenase and the content of reduced glutathione increased， while the contents of Ca²⁺ and malondialdehyde decreased， and the intestinal injury was significantly alleviated. Moreover， the high concentration group （containing 5% H. undatus） had the most significant effect. H. undatus has an alleviating effect on the intestinal injury induced by DSS in Drosophila melanogaster， which may be related to the repair of intestinal cell damage and the enhancement of antioxidant capacity by H. undatus.

Key words: Hylocereus undatus, Drosophila melanogaster, intestinal damage, anti-oxidant

中图分类号:

Q965

韩菊, 陈嘉鹏, 周琪, 陈红蕊, 吴大畅. 火龙果对果蝇肠道损伤的缓解作用研究[J]. 生物技术进展, 2026, 16(2): 372-379.

Ju HAN, Jiapeng CHEN, Qi ZHOU, Hongrui CHEN, Dachang WU. Investigation on the Alleviative Effects of Hylocereus undatus on Intestinal Damage in Drosophila melanogaster[J]. Current Biotechnology, 2026, 16(2): 372-379.

图/表 10

图1 果蝇爬行能力测定结果注：*表示差异在P<0.05水平上具有统计学意义。

Fig. 1 Results of Drosophila locomotor ability assay

图2 果蝇活跃度检测结果注：*表示差异在P<0.05水平上具有统计学意义。

Fig. 2 Results of Drosophila ability

图3 果蝇生存率测定结果

Fig. 3 Results of Drosophila survival rate

图4 CAT活性检测结果注：*表示差异在P<0.05水平上具有统计学意义。

Fig. 4 Results of CAT activity assay

图5 SOD活性检测结果注：*表示差异在P<0.05水平上具有统计学意义。

Fig. 5 Results of SOD activity assay

图6 GSH含量检测结果注：*表示差异在P<0.05水平上具有统计学意义。

Fig. 6 Results of GSH content assay

图7 MDA含量检测结果注：*表示差异在P<0.05水平上具有统计学意义。

Fig. 7 Results of MDA content assay

图8 蓝精灵实验结果

Fig. 8 Smurf assay results

图9 不同组别蓝精灵实验结果注：*表示与其他各组比较，差异在P<0.05水平上具有统计学意义。

Fig. 9 Smurf assay results of different groups

图10 肠上皮细胞损伤检测结果注：*表示与其他各组比较，差异在P<0.05水平上具有统计学意义。

Fig. 10 Results of intestinal epithelial cell injury detection

参考文献 29

[1]	PITMAN R S, BLUMBERG R S. First line of defense: the role of the intestinal epithelium as an active component of the mucosal immune system[J]. J. Gastroenterol., 2000, 35(11): 805-814.
[2]	YUAN Q, WALKER W A. Innate immunity of the gut: mucosal defense in health and disease[J]. J. Pediatr. Gastroenterol. Nutr., 2004, 38(5): 463-473.
[3]	柏超凡,洪梦杰,杨帅,等.人体内肠道菌群功能及调控作用研究进展[J].韶关学院学报,2024,45(9):58-63.
	BAI C F, HONG M J, YANG S, et al.. Research progress on the function and regulation of intestinal flora in human body[J]. J. Shaoguan Univ., 2024, 45(9): 58-63.
[4]	何丽,张永宣,李泊潼,等.模式生物果蝇在中医药现代研究中的优势与应用前景[J].中国中药杂志,2024,49(20):5410-5421.
	HE L, ZHANG Y X, LI B T, et al.. Advantages and application prospects of model organism Drosophila in modern research of traditional Chinese medicine[J]. China J. Chin. Mater. Med., 2024, 49(20): 5410-5421.
[5]	束家宽,余霞,丁成军,等.聚焦生物学核心素养的教学资源剖析:以遗传学模式生物果蝇为例[J].生物学通报,2023,58(11):40-43.
	SHU J K, YU X, DING C J, et al.. Analysis of teaching resources focusing on the key competence of biology: taking Drosophila, a genetic model organism, as an example[J]. Bull. Biol., 2023, 58(11): 40-43.
[6]	APIDIANAKIS Y, RAHME L G. Drosophila melanogaster as a model for human intestinal infection and pathology[J]. Dis. Models Mech., 2011, 4(1): 21-30.
[7]	BRUMBY A M, RICHARDSON H E. Using Drosophila melanogaster to map human cancer pathways[J]. Nat. Rev. Cancer, 2005, 5(8): 626-639.
[8]	郝阳光,张玮钰,金丽华.果蝇肠道免疫的研究进展[J].免疫学杂志,2013,29(9):813-817.
	HAO Y G, ZHANG W Y, JIN L H. Innate immune response of Drosophila intestinal tract[J]. Immunol. J., 2013, 29(9): 813-817.
[9]	BITEAU B, HOCHMUTH C E, JASPER H. Maintaining tissue homeostasis: dynamic control of somatic stem cell activity[J]. Cell Stem Cell, 2011, 9(5): 402-411.
[10]	JIANG H, EDGAR B A. Intestinal stem cells in the adult Drosophila midgut[J]. Exp. Cell Res., 2011, 317(19): 2780-2788.
[11]	王旭旭,马领领,马卓云,等.火龙果的功能及其作用机制研究进展[J].食品工业科技,2019,40(21):352-360.
	WANG X X, MA L L, MA Z Y, et al.. Research progress on the function of pitaya and its mechanism[J]. Sci. Technol. Food Ind., 2019, 40(21): 352-360.
[12]	ZHANG G, GU Y, DAI X. Protective effect of bilberry anthocyanin extracts on dextran sulfate sodium-induced intestinal damage in Drosophila melanogaster [J/OL]. Nutrients, 2022, 14(14): 2875[2026-02-22]. .
[13]	李文佳,刘强,金丽华.刺五加提取物对果蝇免疫功能的影响[J].中草药,2012,43(10):1997-2001.
	LI W J, LIU Q, JIN L H. Effect of extract from roots and rhizomes of Acanthopanax senticosus on immune function of Drosophila melanogaster [J]. Chin. Tradit. Herb. Drugs, 2012, 43(10): 1997-2001.
[14]	别娜娜,王红彩,刘思于,等.二十二碳六烯酸对阿尔茨海默病果蝇寿命的影响[J].食品科学,2025,46(6):156-163.
	BIE N N, WANG H C, LIU S Y, et al.. Effect of docosahexaenoic acid on the lifespan of Drosophila melanogaster with Alzheimer's disease[J]. Food Sci., 2025, 46(6): 156-163.
[15]	ANNA AALTO. Met1-ubiquitination determines inflammatory signalling in Drosophila melanogaster [D]. Finland: Åbo Akademi University, 2022.
[16]	李美英,罗皓琳,王弘,等.基于SDS诱导果蝇肠炎模型的番石榴多糖提取及活性组分评价综合实验设计[J].实验科学与技术,2025,23(6):1-8.
	LI M Y, LUO H L, WANG H, et al.. A comprehensive experimental design for the extraction of guava polysaccharides and the evaluation of active components using a Drosophila enteritis model induced by SDS[J]. Exp. Sci. Technol., 2025, 23(6): 1-8.
[17]	XU W, RUSTENHOVEN J, NELSON C A, et al.. A novel immune modulator IM33 mediates a Glia-gut-neuronal axis that controls lifespan[J]. Neuron, 2023, 111(20): 3244-3254.
[18]	崔海燕,纪龙翔,朱宇晴,等.黄芪多糖对脂多糖诱导肠上皮细胞IPEC-J2氧化应激和炎症反应的缓解作用[J].河南师范大学学报(自然科学版),2022,50(4):101-106.
	CUI H Y, JI L X, ZHU Y Q, et al.. Astragalus polysaccharides alleviate lipopolysaccharide-induced oxidative stress and inflammation in IPEC-J2 cells[J]. J. Henan Norm. Univ. Nat. Sci. Ed., 2022, 50(4): 101-106.
[19]	ESATBEYOGLU T, WAGNER A E, MOTAFAKKERAZAD R, et al.. Free radical scavenging and antioxidant activity of betanin: electron spin resonance spectroscopy studies and studies in cultured cells[J]. Food Chem. Toxicol., 2014, 73: 119-126.
[20]	郭伟灵.产肌苷肠道菌对结肠炎的缓解作用及机制[D].无锡:江南大学,2023.
[21]	魏晶晶.杜仲多糖抗衰老的作用及机制研究[D].杨凌:西北农林科技大学,2023.
[22]	ZHONG D, JIN K, WANG R, et al.. Microalgae-based hydrogel for inflammatory bowel disease and its associated anxiety and depression[J/OL]. Adv. Mater., 2024, 36(24): 2312275[2026-01-22]. .
[23]	KHUITUAN P, SAKENA K, BANNOB K. Prebiotic oligosaccharides from dragon fruits alter gut motility in mice[J]. Biomed. Pharmacother., 2023, 8(12): 598-619.
[24]	DAI X, ZHANG Q, ZHANG G, et al.. Protective effect of agar oligosaccharide on male Drosophila melanogaster suffering from oxidative stress via intestinal microflora activating the Keap1-Nrf2 signaling pathway[J/OL]. Carbohydr. Polym., 2023, 313: 120878[2026-01-11]. .
[25]	YASMIN A, SUMI M J, AKTER K, et al.. Comparative analysis of nutrient composition and antioxidant activity in three dragon fruit cultivars[J/OL]. PeerJ, 2024, 12: e17719[2026-01-11]. .
[26]	SINGH J, NIGAM R, KATIYAR H. A review on nutritional and medicinal potential of dragon fruit (Hylocereus polyrhizus)[J]. Int. J. Adv. Biochem. Res., 2024, 8(11): 214-218.
[27]	SNEHAL A S, SONAL. S S. Dragon fruit: a review of health coverage and nutrients[J]. Int. J. Res. Public. Rev., 2022,13(6): 2693-2702.
[28]	LIAO W, WU H, PANG L, et al.. Hylocereus undatus flower suppresses DSS-induced colitis in mice by reducing intestinal inflammation, repairing the intestinal physical barrier, and modulating gut and lung microbiota[J/OL]. J. Funct. Foods, 2023, 110: 105820[2026-01-11]. .
[29]	朱凯,陈冬生.人类常见疾病与衰老的果蝇模型研究进展[J].激光生物学报,2024,33(4):297-305.
	ZHU K, CHEN D S. Advances in Drosophila models of common human diseases and aging[J]. Acta Laser Biol. Sin., 2024, 33(4): 297-305.

火龙果对果蝇肠道损伤的缓解作用研究

Investigation on the Alleviative Effects of Hylocereus undatus on Intestinal Damage in Drosophila melanogaster

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