生物技术进展 ›› 2018, Vol. 8 ›› Issue (2): 161-168.DOI: 10.19586/j.2095-2341.2017.0154

• 研究论文 • 上一篇    下一篇

响应面法优化玫瑰茄粗多糖提取工艺及其抗氧化活性的研究

郑大恒1,2,王未2,李倩2,陈瑶3,张伟杰2,赵婷4,仰榴青4*   

  1. 1.绍兴文理学院生命科学学院, 浙江 绍兴 312000; 2.江苏大学食品与生物工程学院, 江苏 镇江 212013; 3.江苏大学环境与安全工程学院, 江苏 镇江 212013; 4.江苏大学化学化工学院, 江苏 镇江 212013
  • 收稿日期:2017-11-22 出版日期:2018-03-25 发布日期:2017-12-21
  • 通讯作者: 仰榴青，教授，博士，主要从事天然活性产物提取与应用研究。E-mail:yangliuqing@jsu.edu.cn
  • 作者简介:郑大恒，实验师，硕士，主要从事天然活性产物提取与应用研究。E-mail:zdh@usx.edu.cn。
  • 基金资助:
    江苏省普通高校研究生科研创新计划（CXZZ12-0706）资助。

Optimization on Extraction Technology of Polysaccharides from Hibiscus sabdariffa by Response Surface Methodology and Evaluation of its Antioxidant Activity

ZHENG Daheng, WANG Wei, LI Qian, CHEN Yao, ZHANG Weijie, ZHAO Ting4, YANG Liuqing4   

  1. 1.School of Life Science, Shaoxing University, Zhejiang Shaoxing 312000, China; 2.School of Food and Biological Engineering, Jiangsu University, Jiangsu Zhenjiang 212013, China; 3.School of Environment and Safety, Jiangsu University, Jiangsu Zhenjiang 212013, China; 4.School of Chemistry and Chemical Engineering, Jiangsu University, Jiangsu Zhenjiang 212013, China
  • Received:2017-11-22 Online:2018-03-25 Published:2017-12-21

PDF

摘要： 利用响应面法优化玫瑰茄粗多糖的提取工艺条件,测定粗多糖的抗氧化活性。按照Box-Behnken中心组合试验设计原理，以玫瑰茄粗多糖的得率为响应值，在单因素试验的基础上，进行响应面分析试验，考察料液比、提取时间和提取温度对得率的影响。玫瑰茄粗多糖最佳提取工艺条件为：料液比1∶26 (g/mL)、提取时间3.1 h、提取温度90℃，在该最优条件下所得玫瑰茄粗多糖的得率为14.41%，与预测值接近；玫瑰茄粗多糖对DPPH、羟基、超氧阴离子自由基具有一定的清除作用。研究结果为玫瑰茄粗多糖的研究、开发和利用提供了理论基础。

关键词: 玫瑰茄, 多糖, 响应面分析, 提取工艺优化, 抗氧化

Abstract: In this study, the crude polysaccharide was extracted from Hibiscus sabdariffa, and its extraction process and antioxidant activity were also studied. Based on single factor experiments, the response surface methodology (RSM) was employed to further optimize extraction conditions. The optimal extracted parameters were solid-liquid ratio of 1∶26, extraction time of 3.1 h and the extraction temperature of 90℃. Under these conditions, the yield of the crude polysaccharide was 14.41%, which is closed to the predicted value. Meanwhile, it had certain scavenging effect on DPPH free radical, hydroxyl free radical and superoxidant free radical. This process can be applied in the preparation of the polysaccharide. The results provided a scientific basis for researching, developing and utilization of the polysaccharide from Hibiscus sabdariffa.

Key words: Hibiscus sabdariffa L., polysaccharide, response surface methodology, optimization of extraction process, antioxidant activity

版权所有 © 2021《生物技术进展》编辑部

京ICP备05021913号-17

本系统由北京玛格泰克科技发展有限公司设计开发 技术支持：support@magtech.com.cn