Identification of Powdery Mildew Resistant Genes based on SCAR Markers in Common Bean (Phaseolus vulgaris L.)

doi:10.3969/j.issn.2095-2341.2013.0 .

Curr. Biotech. ›› 2013, Vol. 3 ›› Issue (5): 357-362.DOI: 10.3969/j.issn.2095-2341.2013.0 .

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Identification of Powdery Mildew Resistant Genes based on SCAR Markers in Common Bean (Phaseolus vulgaris L.)

WU Xing-bo, HAO Jun-jie, ZHANG Xiao-yan, WAN Shu-wei, LI Hong-wei, SHAO Yang, SUN Ji-lu

1.Qingdao Academy of Agricultural Sciences, Shandong Qingdao 266100, China;
2.College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China

Received:2013-07-29 Online:2013-09-25 Published:2013-09-06

普通菜豆抗白粉病基因SCAR标记鉴定

吴星波1,2,郝俊杰1*,张晓艳1,万述伟1,李红卫1,邵阳1，孙吉禄1

1.青岛市农业科学研究院, 山东青岛 266100;
2.西南大学园艺园林学院, 重庆 400715

通讯作者: 郝俊杰，助理研究员，博士，研究方向为蔬菜抗病育种。E-mail : haojunj@126.com
作者简介:吴星波，硕士，研究方向为蔬菜分子生物学与基因工程。E-mail :wuxingbo@126.com。
基金资助:
现代农业产业技术体系专项资金资助(CARS-09)；农业部“引进国际先进农业科学技术”项目（2012Z-52；2011-G1（3）--08）；青岛市农科院博士基金项目资助。

Abstract

Abstract: Eight SCAR primer combinations（SAU5, SS18, SF6Em3, SF12R9, SF13R10, SF18R7, SF18R15 and SMe1Em5）of common bean powdery mildew resistant genes were used to check the genome DNA of 78 common bean accessions. The SCAR primer pairs of SF12R9, SF13R10, SF18R7, SF18R15 and SMe1Em5 did not amplified target bands at all; SAU5 marker appeared in 52 accessions, SF6Em3 marker in 66 accessions, and SS18 marker in 76 accessions. 2~3 SCAR markers appeared in 76 accessions respectively. The types of powdery mildew resistant genes in each of the 78 accessions have been identified, and the accessions with pyramiding powdery mildew resistance genes were selected.

Key words: common bean, SCAR, powdery mildew, resistance gene, molecular marker

摘要： 利用8个来自于普通菜豆抗白粉病基因SCAR 标记（SAU5、SS18、SF6Em3、SF12R9、SF13R10、SF18R7、SF18R15和SMe1Em5）引物组，对78份普通菜豆资源进行抗白粉病分子标记的鉴定。结果表明：在这78份资源中，SF12R9、SF13R10、SF18R7、SF18R15和SMe1Em5均无扩增带。有52份资源含有SAU5标记，66份资源含有SF6Em3标记，76份资源含有SS18标记。76份资源含有2~3个标记。该研究明确了78份参试菜豆资源所含的抗白粉病基因类型，并筛选出抗白粉病基因聚合体的参试菜豆资源。

关键词: 普通菜豆, SCAR, 菜豆白粉病, 抗病基因, 分子标记

WU Xing-bo1,2, HAO Jun-jie1*, ZHANG Xiao-yan1, WAN Shu-wei1, LI Hong-wei1, SHAO Yang1, SUN Ji-lu1. Identification of Powdery Mildew Resistant Genes based on SCAR Markers in Common Bean (Phaseolus vulgaris L.)[J]. Curr. Biotech., 2013, 3(5): 357-362.

吴星波,郝俊杰,张晓艳,万述伟,李红卫,邵阳，孙吉禄. 普通菜豆抗白粉病基因SCAR标记鉴定[J]. 生物技术进展, 2013, 3(5): 357-362.

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Identification of Powdery Mildew Resistant Genes based on SCAR Markers in Common Bean (Phaseolus vulgaris L.)

普通菜豆抗白粉病基因SCAR标记鉴定

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