Abstract: Spores and protoplasts of the original strain Streptomyces avermitilis 76-12 were treated with nitrosoguanidine (NTG), acridine orange, ultraviolet radiation (UV) and LiCl respectively. Then a series of high yield avermectin producing and stable mutants were obtained through mutation and rational selection. The avermection productivity of the mutant N-1-2 was 2.47 times of the original strain. Furthermore, several special mutants were obtained. Mutant strain G-32 produced only “a” avermectins; mutant strain Ave8 had higher lever of avermectin B1a content than original strain; and mutant strain UA-G developed bluegreen spores.

Key words: avermectin, streptomyces avermitilis, mutation, rational screening

摘要： 以阿维链霉菌(Streptomyces avermitilis)76-12为出发菌株,采用亚硝基胍、吖啶橙、紫外线和氯化锂分别对其孢子和原生质体进行诱变,经抗代谢物理性筛选,获得一系列高产突变株,其中N-1-2高产突变株的发酵单位是出发菌株的2.47倍。实验中同时获得了只产阿维菌素a组分的突变株G-32、Bla组分含量高的Ave8菌株和产蓝绿色孢子的突变株UA-G等。

关键词: 阿维菌素, 阿维链霉菌, 诱变, 理性筛选