Research Progress on PRNs Gene Family

doi:10.19586/j.2095-2341.2021.190

Current Biotechnology ›› 2022, Vol. 12 ›› Issue (3): 325-331.DOI: 10.19586/j.2095-2341.2021.190

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Research Progress on PRNs Gene Family

Jinrui FAN(), Lei WANG, Junjie ZOU()

Biotechnology Research Institute，Chinese Academy of Agricultural Sciences，Beijing 100081，China

Received:2021-12-06 Accepted:2021-12-17 Online:2022-05-25 Published:2022-05-26
Contact: Junjie ZOU

PRNs基因家族研究进展

樊锦瑞(), 王磊, 邹俊杰()

中国农业科学院生物技术研究所，北京 100081

通讯作者: 邹俊杰
作者简介:樊锦瑞 E-mail：fanjinrui1997@163.com；
基金资助:
国家自然科学基金项目(32070337);北京市自然科学基金项目(6192025);中央级公益性科研院所基本科研业务费专项(1610392020001)

Abstract

Abstract:

Pirins （PRNs， PIRs） are gene family containing cupin domain and belong to the functionally diversity cupin superfamily. The N-terminal domain of PRN contains a metal-ion binding site， which is highly conserved in prokaryotes and eukaryotes. Human hPRN could participate in cancer development as transcriptional regulator and redox sensor， suggesting that hPRN is a potential therapeutic target. In plants， there are several members in PRNs gene family， whereas their biological functions remain largely unknown. This paper outlined the basic structure and biochemical characteristic of PRNs， and reviewed the biological research progresses in human， microorganism and plants， in order to provide clues to clarify the PRNs molecular mechanism of crop such as rice， and provide target genes for the genetic improvement of crops production or adapation by gene editing and other molecular breeding techniques.

Key words: PRNs gene family, metal ions, transcriptional regulator, redox sensor, target

摘要：

Pirins（简称PRNs、PIRs）是含有Cupin结构域的基因家族，属于功能各异的Cupin超家族成员之一。PRN蛋白的N端结构域含有金属离子结合位点，在原核和真核生物中高度保守。人hPRN可作为转录因子辅因子和氧化还原感受器参与癌症发生过程，是癌症治疗的潜在靶点。植物PRNs基因家族一般含有多个成员，其生物学功能尚未得到深入研究。概述了PRNs蛋白的基本结构及其生化特征，并综述了PRNs在人、微生物和植物中的生物学功能，以期为进一步解析水稻等作物中PRNs的功能提供一定线索，并为基因编辑等生物育种技术改良作物提供新的靶点。

关键词: PRNs基因家族, 金属离子, 转录因子辅因子, 氧化还原感受器, 靶点

CLC Number:

Q501

Jinrui FAN, Lei WANG, Junjie ZOU. Research Progress on PRNs Gene Family[J]. Current Biotechnology, 2022, 12(3): 325-331.

樊锦瑞, 王磊, 邹俊杰. PRNs基因家族研究进展[J]. 生物技术进展, 2022, 12(3): 325-331.

Figures/Tables 2

References 43

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物种	基因名	生物学功能	文献来源
人类	hPRN	与Fe²⁺结合，具有槲皮素酶活性，可降解槲皮素	[4,9]
		参与吸烟引起的上皮细胞凋亡，表达受核因子Nrf2调节	[1920]
		参与黑色素肿瘤细胞迁移，抑制黑素细胞衰老	[2123]
		表达受miR⁃455⁃5p调节，下调后抑制前列腺癌细胞的迁移和侵袭	[24]
		通过激活E2F1及其靶基因刺激乳腺癌肿瘤的发生	[25]
		末期髓细胞成熟所必需，下调有助于急性髓系白血病相关的分化停滞	[26]
		参与真皮纤维化过程	[27]
		下调Eaf2/U19信号转导并延缓其对前列腺癌细胞的生长抑制作用	[28]
		作为Fe²⁺/Fe³⁺依赖的氧化还原感受器，氧化态结合核因子NF-κB，调控下游基因表达	[2，2930]
沙雷氏菌	pirin_Sm	与丙酮酸脱氢酶E1亚基互作调节丙酮酸脱氢酶活性，调节丙酮酸的分解代谢	[31]
链霉菌	pirA	突变体对氧化损伤和聚酮化合物抗生素产生的抑制高度敏感，可催化β-氧化途径的第一步	[32]
大肠杆菌	YhhW	具有槲皮素酶活性，可降解槲皮素，与Ni²⁺结合	[4,9]
蓝藻	prnA	受盐、渗透等胁迫诱导上调	[33]
斯氏假单胞菌	pirin⁃like	与Cu²⁺结合，具有槲皮素酶活性	[12]
番茄	LePirin	参与细胞程序性死亡	[34]
拟南芥	AtPRN1	通过GCR1、GPA1、PRN1、NF-Y信号通路介导蓝光和脱落酸反应	[3,14]
		突变体对不同真菌敏感性不一样	[15]
		具有槲皮素酶活性，参与种子萌发时对光/暗响应	[1617]
	AtPRN2	稳定半胱氨酸蛋白酶XCP2，并增加对维管病原菌青枯雷尔氏菌的敏感性，突变体对该菌不敏感	[18]
	AtPRN2	抑制拟南芥木质部中的S型木质素积累	[13]

物种	基因名	生物学功能	文献来源
人类	hPRN	与Fe²⁺结合，具有槲皮素酶活性，可降解槲皮素	[4,9]
		参与吸烟引起的上皮细胞凋亡，表达受核因子Nrf2调节	[1920]
		参与黑色素肿瘤细胞迁移，抑制黑素细胞衰老	[2123]
		表达受miR⁃455⁃5p调节，下调后抑制前列腺癌细胞的迁移和侵袭	[24]
		通过激活E2F1及其靶基因刺激乳腺癌肿瘤的发生	[25]
		末期髓细胞成熟所必需，下调有助于急性髓系白血病相关的分化停滞	[26]
		参与真皮纤维化过程	[27]
		下调Eaf2/U19信号转导并延缓其对前列腺癌细胞的生长抑制作用	[28]
		作为Fe²⁺/Fe³⁺依赖的氧化还原感受器，氧化态结合核因子NF-κB，调控下游基因表达	[2，2930]
沙雷氏菌	pirin_Sm	与丙酮酸脱氢酶E1亚基互作调节丙酮酸脱氢酶活性，调节丙酮酸的分解代谢	[31]
链霉菌	pirA	突变体对氧化损伤和聚酮化合物抗生素产生的抑制高度敏感，可催化β-氧化途径的第一步	[32]
大肠杆菌	YhhW	具有槲皮素酶活性，可降解槲皮素，与Ni²⁺结合	[4,9]
蓝藻	prnA	受盐、渗透等胁迫诱导上调	[33]
斯氏假单胞菌	pirin⁃like	与Cu²⁺结合，具有槲皮素酶活性	[12]
番茄	LePirin	参与细胞程序性死亡	[34]
拟南芥	AtPRN1	通过GCR1、GPA1、PRN1、NF-Y信号通路介导蓝光和脱落酸反应	[3,14]
		突变体对不同真菌敏感性不一样	[15]
		具有槲皮素酶活性，参与种子萌发时对光/暗响应	[1617]
	AtPRN2	稳定半胱氨酸蛋白酶XCP2，并增加对维管病原菌青枯雷尔氏菌的敏感性，突变体对该菌不敏感	[18]
	AtPRN2	抑制拟南芥木质部中的S型木质素积累	[13]

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Research Progress on PRNs Gene Family

PRNs基因家族研究进展

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