Abstract: A large number of animal experiments and clinical trials have shown the potential positive effects of hydrogen on the prevention or treatment of diseases. Although the safety of hydrogen has been widely recognized at present, there are still a lack of experimental data supporting whether long-term hydrogen intervention affects the physiological function of the body. In order to explore the effects of long-term drinking of hydrogen-rich water (HRW) on the physiological function, we elaborated the body and organ weight of rats, the changes of water, food intake, feces and urine excretions, and the cardiac function. Besides, the blood indicators were also monitored. The results were as follows: HRW had no significant effects on the blood routine indicators, fasting blood glucose and the liver function index. The serum triglyceride level of rats in HRW group was significantly elevated (P＜0.05), but there were no significant differences in cholesterol, total bile acid and uric acid between two groups. HRW had no significant effects on the cardiac function of rats. The brain weight of rats increased markedly in HRW group in comparison with the control group (P＜0.05); HRW had no significant effects on body and other organs weight, water and food intake as well as faeces and urine excretions. We observed the long-term effects of HRW intervention, which is of basic research value and clinical significance.

Key words: hydrogen-rich water, long-term intervention, physiological function, brain, triglyceride

摘要： 大量动物实验和临床试验显示，氢气可能对疾病的预防或治疗有积极作用。尽管目前氢气的安全性已被广泛认可，但是长期氢气干预是否会对机体的生理功能产生影响还缺乏实验数据支持。为了探究长期饮用富氢水（hydrogen-rich water, HRW）对生理功能的影响，测试了长期饮用富氢水大鼠的体重、脏器重量、饮水进食及排泄量、心脏功能、血常规和血清生化指标。研究发现：富氢水对血常规/空腹血糖/肝功能无显著影响；富氢水能够显著提高大鼠血清甘油三酯水平（P＜0.05），而胆固醇、胆汁酸和尿酸水平无显著变化；富氢水对大鼠心脏功能无显著影响；富氢水能够显著提高大鼠脑组织重量（P＜0.05）；富氢水对大鼠体重、其他脏器重量、饮食进水量和排泄量无显著影响。对富氢水干预后的长期影响效果的观察具有一定的基础研究价值和临床参考意义。

关键词: 富氢水, 长期干预, 生理功能, 脑, 甘油三酯