Curr. Biotech. ›› 2014, Vol. 4 ›› Issue (3): 207-210.DOI: 10.3969/j.issn.2095-2341.2014.0 .

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Mutation Breeding of Vitamin B1 Auxotrophy of Propionibacteria freudenreichii

CHUAI Yu-duo, MA Zhi-gang, WANG De-pei   

  1. 1.Tianjin Modern Vocational Technology College, Tianjin 300350, China;
    2.College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China
  • Received:2014-03-21 Online:2014-05-25 Published:2014-04-11

费氏丙酸菌维生素B1营养缺陷型菌株的诱变选育

揣玉多1,马志刚2,王德培2   

  1. 1.天津现代职业技术学院, 天津 300350;
    2.天津科技大学生物工程学院, 天津 300457
  • 作者简介:揣玉多，讲师，硕士，主要从事菌种选育和发酵饲料的研究。E-mail：cyd0707@126.com

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Abstract: Acetic acid is one of main by-products in propionic acid fermentation. Vitamin B1 is involved in acetic acid biosynthesis. The vitamin B1 auxotrophy would reduce the acetic acid biosynthesis, which result in increase of propionic acid yield. A vitamin B1 auxotrophy mutant Pf007 was screened out from Propionibacteria freudenreichii IFFI.10019 by UV treatment，and the propionic acid production level reached up to 2.1 g/L，which increased 91% compared with original strain's 1.1 g/L.

Key words: propionic acid, mutation, auxotrophy

摘要： 在丙酸发酵过程中，乙酸为主要副产物，维生素B1与乙酸合成途径相关，筛选维生素B1营养缺陷型突变株有助于降低乙酸的合成，提高丙酸的产量。以费氏丙酸菌IFFI.10019作为出发菌株，经过紫外线诱变处理，筛选育种获得维生素B1营养缺陷型费氏丙酸菌二株，其中Pf007菌株的丙酸产量由原来的1.1 g/L提高到2.1 g/L，提高率达到91%。

关键词: 丙酸, 诱变, 营养缺陷型

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