Identification and Phylogenetic Analysis of Virus Species Infecting Ornamental Jasminum sambae in Fuzhou Region

doi:10.19586/j.2095-2341.2025.0115

Current Biotechnology ›› 2025, Vol. 15 ›› Issue (5): 874-881.DOI: 10.19586/j.2095-2341.2025.0115

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Identification and Phylogenetic Analysis of Virus Species Infecting Ornamental Jasminum sambae in Fuzhou Region

Xiangguo LIU¹(), Yujie JIN², Weimin LI³, Ying FU¹, Jing CHEN⁴, Hongrong HE⁴, Min LI⁵, Meiling HU⁵, Fangluan GAO², Jianguo SHEN⁵()

^1.Fuzhou Jinshan Park Management Office，Fuzhou 350007，China
^2.Institute of Plant Virology，Fujian Agriculture and Forestry University，Fuzhou 350002，China
^3.Department of Plant Protection，Beijing University of Agriculture，Key Laboratory for Northern Urban Agriculture Ministry of Agriculture and Rural Afairs，Beijing 102206，China
^4.Fuzhou Garden Center，Fuzhou 350007，China
^5.Fujian Key Laboratory for Technology Research of Inspection and Quarantine，Technology Center of Fuzhou Customs District，Fuzhou 350001，China

Received:2025-08-28 Accepted:2025-09-18 Online:2025-09-25 Published:2025-11-11
Contact: Jianguo SHEN

Abstract

Abstract:

Jasminum sambac is an important ornamental plant in China and the city flower of Fuzhou. To identify viral pathogens infecting ornamental jasmine in Fuzhou， virus-suspected jasmine samples from parks in Fuzhou were tested using RT-PCR， and the virus gene sequences were subjected to phylogenetic analysis. Results revealed four viruses in 80 tested sample， jasmine virus A （JaVA）， jasmine virus C （JaVC）， jasmine virus H （JaVH） and jasmine virus T （JaVT）， with detection rates of 18.75%， 51.25%， 30.0% and 21.25%， respectively. Mixed infections were observed in 30.0% samples， with nine different combination patterns showing following frequencies： JaVA+JaVC （3.75%）， JaVA+JaVH （1.25%）， JaVC+JaVH （5.0%）， JaVC+JaVT （2.5%）， JaVT+JaVH （1.25%）， JaVA+JaVC+JaVH （1.25%）， JaVA+JaVC+JaVT （1.25%，）， JaVC+JaVH+JaVT （8.75%）， and JaVA+JaVC+JaVH+JaVT （5.0%）. Sequence analysis showed that the Fuzhou isolates from ornamental jasmine share 98.83%~99.48%， 79.64%~95.29%， 85.84%~97.94% and 92.59%~97.85% nucleotide identity with the respective reference sequences in GenBank for JaVA， JaVC， JaVH and JaVT. Phylogenetic analysis indicated that the JaVC isolates obtained in this study were most closely related to the reported isolate TZS （GenBank accession number： ON241322）， the JaVH isolates were most closely related to the reported isolate Jiangsu （GenBank accession number： MH231179）， and the JaVT isolates were most closely related to the reported isolate YJ （GenBank accession number： MZ962345）. The main virus species and their co-infection characteristics of ornamental jasmine in Fuzhou were reported for the first time， which provids baseline data for the prevention and control of jasmine virus disease.

Key words: Jasminum sambae, jasmine A virus, jasmine C virus, jasmine H virus, jasmine T virus

摘要：

茉莉花（Jasminum sambac）是我国重要的观赏花卉，同时也是福州市市花。为明确福州地区观赏茉莉花易感染的主要病毒种类，利用RT-PCR方法对福州市主要公园采集的疑似感染病毒病的茉莉花样品进行检测，并对病毒基因序列进行系统发育分析。结果表明，福州地区的80份茉莉花疑似病样中共检测出茉莉花A病毒（jasmine virus A，JaVA）、茉莉花C病毒（jasmine virus C，JaVC）、茉莉花H病毒（jasmine virus H，JaVH）和茉莉花T病毒（jasmine virus T，JaVT）4种病毒，检出率分别为18.75%、51.25%、30.0%和21.25%。其中，30.0%的样品存在复合侵染现象，分别为JaVA+JaVC（3.75%）、JaVA+JaVH（1.25%）、JaVC+JaVH（5.0%）、JaVC+JaVT（2.5%）、JaVT+JaVH（1.25%）、JaVA+JaVC+JaVH（1.25%）、JaVA+JaVC+JaVT（1.25%）、JaVC+JaVH+JaVT（8.75%）和JaVA+JaVC+JaVH+JaVT（5.0%）。JaVA、JaVC、JaVH、JaVT福州茉莉花分离物序列与GenBank已报道的各病毒序列一致性分别为98.83%~99.48%、79.64%~95.29%、85.84%~97.94%和92.59%~97.85%。系统发育分析结果显示，获得的JaVC分离物与已报道的TZS分离物（GenBank登录号：ON241322）亲缘关系最近；JaVH分离物与已报道的Jiangsu分离物（GenBank登录号：MH231179）亲缘关系最近；JaVT分离物与已报道的YJ分离物（GenBank登录号：MZ962345）亲缘关系最近。首次报道了福州市观赏茉莉花的主要病毒种类及其复合侵染特征，为茉莉花病毒病的监测与防控提供了理论依据。

关键词: 茉莉花, 茉莉A病毒, 茉莉C病毒, 茉莉H病毒, 茉莉T病毒

CLC Number:

Q945.8

Xiangguo LIU, Yujie JIN, Weimin LI, Ying FU, Jing CHEN, Hongrong HE, Min LI, Meiling HU, Fangluan GAO, Jianguo SHEN. Identification and Phylogenetic Analysis of Virus Species Infecting Ornamental Jasminum sambae in Fuzhou Region[J]. Current Biotechnology, 2025, 15(5): 874-881.

刘向国, 金雨洁, 李为民, 付影, 陈婧, 何红榕, 李敏, 胡美玲, 高芳銮, 沈建国. 侵染中国福州观赏茉莉花的病毒种类鉴定及其进化分析[J]. 生物技术进展, 2025, 15(5): 874-881.

Figures/Tables 6

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引物名称	上下游引物序列（5′→3′）	退火温度/℃	目的片段/bp	参考文献
JaVA-(765)-F	5′-ATGAGTAACTCTGAAGGTGA-3′	42	765	—
JaVA-(765)-R	5′-TTAATTTACGTTAGACGCAAG-3′	42	765	—
JaVC-(891)-F	5′-ATGAGTTCCACATCGGATGAC-3′	48	891	—
JaVC-(891)-R	5′-TCATCGTTTCCAAATCCCTCT-3′	48	891	—
JaVH-(1020)-F	5′-ATGGCAGCCAGTGATAGTCC-3′	46	1 020	—
JaVH-(1020)-R	5′-TTACATTCTAGTCACGTACAGAC-3′	46	1 020	—
JaVT-(837)-F	5′-TCGGGTGAAGAGAAAGTCGTTG-3′	52	837	—
JaVT-(837)-R	5′-CAGACCACGCACGCCCAT-3′	52	837	—
TSV-F	5′-ATGAATACTTTGATCCAAGGT-3′	56	717	[9]
TSV-R	5′-TCAGTCTTGATTCACCAGG-3′	56	717	[9]
JMaV-2646F	5′-ACTTGCAGCCACTGGAGTT-3′	57	989	[9]
JMaV-3614R	5′-CTCACATTCTAGTTACGTACA-3′	57	989	[9]
CMV-PuF	5′-TCTCATGGATGCTTCTCCGCG-3′	60	880	[9]
CMV-PuR	5′-CCGTAAGCTGGATGGACAACC-3′	60	880	[9]
ArMV-F	5′-TTGGCAGCGGATTGGGAGTT-3′	52	370	[16]
ArMV-R	5′-ATTGGTTCCAGTTGTTAGTGAC-3′	52	370	[16]
TRSV-F	5′-TATTATGACGCCAACACTCT-3′	53	437	[20]
TRSV-R	5′-AGTATCAGACCTCCAACCA-3′	53	437	[20]
NLV-F	5′-CGAACAAAGCAAGCGAACT-3′	52	829	[16]
NLV-R	5′-AAGATAGGGCCTCTGGTCAAC-3′	52	829	[16]

引物名称	上下游引物序列（5′→3′）	退火温度/℃	目的片段/bp	参考文献
JaVA-(765)-F	5′-ATGAGTAACTCTGAAGGTGA-3′	42	765	—
JaVA-(765)-R	5′-TTAATTTACGTTAGACGCAAG-3′	42	765	—
JaVC-(891)-F	5′-ATGAGTTCCACATCGGATGAC-3′	48	891	—
JaVC-(891)-R	5′-TCATCGTTTCCAAATCCCTCT-3′	48	891	—
JaVH-(1020)-F	5′-ATGGCAGCCAGTGATAGTCC-3′	46	1 020	—
JaVH-(1020)-R	5′-TTACATTCTAGTCACGTACAGAC-3′	46	1 020	—
JaVT-(837)-F	5′-TCGGGTGAAGAGAAAGTCGTTG-3′	52	837	—
JaVT-(837)-R	5′-CAGACCACGCACGCCCAT-3′	52	837	—
TSV-F	5′-ATGAATACTTTGATCCAAGGT-3′	56	717	[9]
TSV-R	5′-TCAGTCTTGATTCACCAGG-3′	56	717	[9]
JMaV-2646F	5′-ACTTGCAGCCACTGGAGTT-3′	57	989	[9]
JMaV-3614R	5′-CTCACATTCTAGTTACGTACA-3′	57	989	[9]
CMV-PuF	5′-TCTCATGGATGCTTCTCCGCG-3′	60	880	[9]
CMV-PuR	5′-CCGTAAGCTGGATGGACAACC-3′	60	880	[9]
ArMV-F	5′-TTGGCAGCGGATTGGGAGTT-3′	52	370	[16]
ArMV-R	5′-ATTGGTTCCAGTTGTTAGTGAC-3′	52	370	[16]
TRSV-F	5′-TATTATGACGCCAACACTCT-3′	53	437	[20]
TRSV-R	5′-AGTATCAGACCTCCAACCA-3′	53	437	[20]
NLV-F	5′-CGAACAAAGCAAGCGAACT-3′	52	829	[16]
NLV-R	5′-AAGATAGGGCCTCTGGTCAAC-3′	52	829	[16]

Identification and Phylogenetic Analysis of Virus Species Infecting Ornamental Jasminum sambae in Fuzhou Region

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病毒	阳性样品数	检出率/%	采样地点（公园）
JaVA	15	18.75	金山、于山、闽江、流花溪、花海
JaVC	41	51.25	金山、于山、乌山、西湖、闽江、乌龙江、流花溪、茶亭、花海、光明港、国光
JaVH	24	30.00	金山、闽江、乌龙江、西湖、流花溪、茶亭、光明港、黎明湖、国光
JaVT	17	21.25	金山、于山、乌山、闽江、乌龙江、流花溪、茶亭、花海、光明港
JaVA+JaVC	3	3.75	金山、流花溪
JaVA+JaVH	1	1.25	流花溪
JaVC+JaVH	4	5.00	于山、茶亭、光明港
JaVC+JaVT	2	2.50	花海
JaVT+JaVH	1	1.25	茶亭
JaVA+JaVC+JaVH	1	1.25	流花溪
JaVA+JaVC+JaVT	1	1.25	金山
JaVC+JaVH+JaVT	7	8.75	乌龙江、流花溪、花海、光明港
JaVA+JaVC+JaVH+JaVT	4	5.00	金山、闽江、花海