Abstract:

The objective of this study was to explore the impact of nano-bubble hydrogen water （NBHW） pretreatment on the initiation and progression of imiquimod （IMQ）-induced psoriasis in mice， as well as the underlying mechanisms. Eight-week-old male BALB/c mice were randomly assigned to five groups： control （CON）， normal saline （NS）， NBHW， calcipotriol （CLO）， and a combination of CLO and NBHW （CLO+NBHW）. After pretreatment， the psoriasis model was established by administering 5% IMQ cream on the shaved back skin and ears， while maintaining the respective treatments. The evolution of skin lesions was documented， and the severity was assessed using the psoriasis area and severity index （PASI）. Hematoxylin-eosin （HE） staining was employed to examine skin tissue sections， evaluating the degree of acanthosis. The spleen index of each group was statistically analyzed， and serum oxidation levels were determined using a malondialdehyde （MDA） kit. Additionally， ELISA was utilized to detect the expression levels of inflammatory factors in serum and skin lesions. The results demonstrated successful induction of psoriasis in all groups. Notably， the NBHW group exhibited less severe inflammation and histopathological alterations compared to the NS and CON groups. Pretreatment with NBHW significantly mitigated the severity of inflammation and histopathological changes in the IMQ-induced mouse model. Additionally， the expression of inflammatory factors in tissues， such as the NBHW group， was downregulated， and the MDA level was lower than in the CON group. These findings indicated that NBHW exhibit a significant protective effect against IMQ-induced psoriasis in mice， not only improving skin lesions but also reducing the production of inflammatory factors and the expression of oxidative markers.

Key words: psoriasis, nanobubble hydrogen water, inflammation, oxidative stress

摘要：

为了探讨纳米气泡氢水（nano-bubble hydrogen water, NBHW）处理对咪喹莫特(imiquimod, IMQ)诱导的小鼠银屑病的发生和发展的影响以及其作用机制，使用8周龄的雄性BALB/C小鼠，将其随机分为5组，空白组（control，CON）、生理盐水组(normal saline，NS）、NBHW组、卡泊三醇组（clobetasol propionate，CLO）以及联合组（CLO+NBHW）。处理后在小鼠维持原处理同时开始造模，背部去毛皮肤处以及耳朵处涂5% IMQ乳膏。记录小鼠皮损变化，用（psoriasis area and severity index，PASI）银屑病皮损评分评估小鼠的皮损表现。同时采用苏木精-伊红（hematoxylin-eosin，HE）染色法对皮肤组织切片进行染色,并对棘层肥厚程度进行评估，并统计分析各组小鼠脾脏指数情况。此外,利用丙二醛(malondialdehyde，MDA)试剂盒测定血清中的氧化指标，ELISA法检测炎症因子在血清及小鼠背部皮损中的表达水平。评估NBHW对这些炎性细胞因子基因表达的影响。结果发现，各组银屑病小鼠造模成功, NBHW组炎症情况和组织病理学变化较NS组和CON组轻微。NBHW处理可以明显减轻咪喹莫特诱发模型小鼠炎症情况和组织病理变化的严重程度。相对于CON组,NBHW组等组织中炎症因子下调；MDA水平低于CON组。结果表明，NBHW对于咪喹莫特诱导的银屑病小鼠模型表现出显著的防治效果，不仅在皮肤病变的改善上有明显作用，还能够降低炎症因子的产生及氧化指标的表达。

关键词: 银屑病, 纳米气泡氢水, 炎症, 氧化应激