Abstract:

Hydrogen molecule is a new type of gas signaling molecule， which has a certain effect on a variety of inflammation diseases. Psoriasis is a chronic， inflammatory disease， and molecular hydrogen is expected to be a new adjuvant therapy for psoriasis. The psoriasis model of mice was induced by 5% imquimod （IMQ） cream for 7 consecutive days， during which the mice were exposed to 35% hydrogen molecular environment twice a day for 2 h each time for intervention. The changes of skin lesions were observed daily and the psoriasis area and severity index （PASI） score was performed. On the 8th day， the skin lesions were taken by death， hematoxylin-eosin （HE） staining， and the histopathological lesion was evaluated using Baker score. The expressions of inflammatory cytokines IL-6， TNF-α and IFN-γ were detected by immunohistochemistry. Real-time quantitative fluorescent PCR （RT-qPCR） and Western blot were used to detect the relative mRNA and protein levels of inflammatory cytokines IL-6， TNF-α and IFN-γ in skin lesions. The content of oxidation index malondialdehyde （MDA） and the activity of antioxidation-related catalase （CAT） were detected by the kit. Results showed that， molecular hydrogen intervention decreased PASI score （P<0.05） and Baker score （P<0.001）， and improved histopathological changes of IMQ-induced mice. The mRNA and protein expression levels of IL-6， TNF-α and IFN-γ in the skin tissue of mice in the hydrogen molecular intervention group were decreased， the content of MDA was decreased， and the activity of CAT was increased （all P<0.05）. The results indicated that， molecular hydrogen can inhibit the formation of psoriasis skin lesions， reduce inflammation and oxidative stress damage， which has the potential to treat psoriasis.

Key words: hydrogen molecular, psoriasis-like dermatitis, oxidative stress, inflammatory response

摘要：

氢分子是一种新型的气体信号分子，对多种炎症疾病有一定的作用效果。银屑病是一种慢性炎症性疾病，氢分子有望作为银屑病辅助治疗的新手段。连续7 d使用5%咪喹莫特（imiquimod, IMQ）乳膏诱导小鼠银屑病模型，期间每天2次、每次2 h将小鼠置于35%的氢分子环境中进行干预。每天观察小鼠皮损变化并进行银屑病面积和严重程度指数（psoriasis area and severity index, PASI）评分；第8天，处死小鼠取皮损，苏木精-伊红（hematoxylin-eosin，HE）染色并使用Baker评分评估组织病理学损害程度；免疫组化检测皮损中炎症因子IL-6、TNF-α和IFN-γ的表达；实时荧光定量PCR（real-time quantitative fluorescent polymerase chain reaction, RT-qPCR）和蛋白免疫印迹检测皮损中炎症因子IL-6、TNF-α和IFN-γ的相对mRNA水平和蛋白水平的表达；用试剂盒检测皮损组织中氧化指标丙二醛（malondialdehyde, MDA）含量和抗氧化相关的过氧化氢酶（catalase, CAT）活力。结果显示，氢分子干预降低了IMQ诱导小鼠的PASI评分（P<0.05）和Baker评分（P<0.001），改善了组织的病理学变化；氢分子干预组小鼠皮损组织中IL-6、TNF-α和IFN-γ的mRNA及蛋白表达水平均下降，MDA的含量下降，CAT的活力增加（P<0.05）。结果表明，氢分子可抑制银屑病样皮损的形成、减轻炎症反应、减轻氧化应激损伤，有望成为治疗和缓解银屑病的新方法。

关键词: 氢分子, 银屑病样皮损, 氧化应激, 炎症反应