Abstract:

To investigate the expression of ribonucleo protein NHP2 in hepatocellular carcinoma （HCC） and to explore the relationship with disease progression and prognosis. Differentially expressed genes of HCC were screened by Quick Go and GEPIIA 2 online databases， and NHP2 was identified as object of study. Furthermore， bioinformatics methods such as STRING， TIMER 2.0， UALCAN database were used to analyze the differential expression of NHP2 in pancreatic cancer and its correlation with pathological progress in HCC and other pancreatic cancers， and to predict the relationship between prognosis and survival. The target miRNAs and lncRNAs were analysed with the miRNet database， the possible regulatory network of CeRNAs was mapped by Cytescape v3.8.0. Results showed that， 113 genes related to cell aging and 2 206 differentially expressed genes（DEGs） in HCC were found in the online database. 19 DEGs were involved in the biological process of cell aging in HCC. Among them， NHP2 is significantly highly expressed in a variety of cancers including HCC， and the prognosis of HCC with high NHP2 expression is poor in statistical difference. There are 10 interacting proteins encoded by the NHP2 gene， it is mainly involved in 1 signalling pathway （KEGG）， 6 molecular functions （MF）， 11 cellular components （CC） and 7 biological processes （BP）. Two NHP2-targeting miRNAs and 57 NHP2-targeting lncRNAs had been predicted. The results indicated that， NHP2 is highly expressed in a variety of tumors， including HCC， and is significantly different in age and stage of patients with a variety of tumors. During the proliferation and senescence of HCC， it may be regulated by the regulatory axis of lncRNAs/miR-1-3p/NHP2 or lncRNAs/miR-124-3p/NHP2 and be a prognostic predictor and a potential therapeutic target of HCC.

Key words: NHP2, cellular senescence, hepatocellular carcinoma, bioinformatics

摘要：

为了探讨核糖核蛋白NHP2在肝细胞癌（hepatocellular carcinoma, HCC）中的表达情况，了解其与疾病进展与预后的关系，通过Quick Go、GEPIA 2在线数据库筛选HCC细胞衰老的差异表达基因并确定了研究对象基因NHP2；进一步使用STRING、TIMER 2.0、UALCAN数据库等生物信息学方法分析NHP2在泛癌中的差异表达以及在肝癌和其他泛癌中病理进展过程中的相关性，并预测预后生存关系；使用miRNet数据库分析其靶向miRNAs和lncRNAs，应用Cytoscape v3.8.0绘制可能的CeRNAs调控网络图。结果显示，在线数据库检索到细胞衰老相关生物学过程相关基因113个，HCC差异表达基因2 206个，共有19个差异表达基因参与了肝癌细胞衰老的生物学过程。其中，NHP2在包括HCC的多种癌症中显著高表达，NHP2高表达的肝癌人群预后较差，具有统计学差异。NHP2基因编码的互作蛋白有10个，主要参与了1个信号通路（KEGG信号通路）、6个分子功能（molecular function，MF）、11个细胞组分（cellular component，CC）和7个生物学过程（biological process，BP）。结果预测出NHP2靶向miRNAs有2个，lncRNAs有57个。研究结果表明，NHP2在包括HCC的多种肿瘤中高表达，且根据患者的年龄、分期等状态有明显差异，在HCC增殖和衰老过程中，可能通过lncRNAs/miR-1-3p/NHP2或lncRNAs/miR-124-3p/NHP2调控轴进行调节，是HCC预后的预测因子和潜在治疗靶点。

关键词: 核糖核蛋白, 细胞衰老, 肝细胞癌, 生物信息学