Metabolomics and its Application in Environmental Toxicology

doi:10.19586/j.2095-2341.2022.0057

Current Biotechnology ›› 2022, Vol. 12 ›› Issue (5): 683-689.DOI: 10.19586/j.2095-2341.2022.0057

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Metabolomics and its Application in Environmental Toxicology

Jiayi LIN(), Ju XIE, Yueping ZHU(), Wenyu XIE

Guangdong Provincial Key Laboratory of Petrochemical Pollution Process and Control，Key Laboratory of Petrochemical Pollution Control of Guangdong Higher Education Institutes，Guangdong University of Petrochemical Technology，Guangdong Maoming 525000，China

Received:2022-04-14 Accepted:2022-07-12 Online:2022-09-25 Published:2022-09-30
Contact: Yueping ZHU

代谢组学及其在环境毒理学中的应用

林嘉意(), 谢炬, 朱越平(), 谢文玉

广东石油化工学院，广东省石油化工污染过程与控制重点实验室，广东高校石油化工污染控制重点实验室，广东茂名 525000

通讯作者: 朱越平
作者简介:林嘉意 E-mail：lero_lin@163.com；
基金资助:
茂名市科技计划项目(2020KJZX031)

Abstract

Abstract:

As a part of system biology， metabolomics is widely used in biomedical research because of its fast analysis. At present， the studies of metabolomics in environmental toxicology mainly focus on single pollutants， and the complex situation of contaminated areas should also be considered. The application of metabolomics in environmental toxicology was reviewed by introducing metabolomics and its development process. We also summarized the respective characteristics of the current mainstream metabolomics technology， and discussed the research and application progress of metabolomics in the toxicity evaluation of environmental heavy metals， organic pollutants and antibiotics， and environmental stress tolerance evaluation. At last， we pointed point out that the application of metabolomics in environmental toxicology needs to be strengthened. The paper was expected to provide reference for the application reasearch of metabonomics in environmental toxicology

Key words: metabonomics, environmental toxicology, heavy metals, organic pollutants, antibiotics

摘要：

代谢组学作为系统生物学的一部分，因其具有分析速度快的特点，被广泛用于生物医学等方面的研究。目前代谢组学在环境毒理学方面的研究主要针对单一污染物，但也需要考虑到被污染地的复杂情况。通过介绍代谢组学及其发展历程，总结了目前主流代谢组学技术的各自特点，讨论了代谢组学在环境重金属、有机污染物和抗生素中的毒性评估以及环境胁迫耐受性中的评价等方面内容，综述了其在环境毒理学中的应用，并指出其应用不足，旨在为代谢组学应用于环境毒理学的研究提供参考。

关键词: 代谢组学, 环境毒理学, 重金属, 有机污染物, 抗生素

CLC Number:

Q959

Jiayi LIN, Ju XIE, Yueping ZHU, Wenyu XIE. Metabolomics and its Application in Environmental Toxicology[J]. Current Biotechnology, 2022, 12(5): 683-689.

林嘉意, 谢炬, 朱越平, 谢文玉. 代谢组学及其在环境毒理学中的应用[J]. 生物技术进展, 2022, 12(5): 683-689.

Figures/Tables 2

References 48

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方法	取样特点	优点	缺点	参考文献
NMR	预处理简单、无偏向、范围广、无创性	良好的重现性、较高通量和较低检测成本	灵敏度低、分辨率低、需要大量样品、仪器所需的投资大	[6-7]
¹H-NMR	预处理简单、无偏向、范围广、无创性	样品用量少可回收、分析时间短、准确度高、重复性好、易制备	灵敏度低、峰信号重叠	[8，21]
¹³C-NMR	预处理要求低、一些含有矿物质的样品需要做脱矿处理	可获得结构上的细微变化	容易产生干扰峰	[10，22]

方法	取样特点	优点	缺点	参考文献
NMR	预处理简单、无偏向、范围广、无创性	良好的重现性、较高通量和较低检测成本	灵敏度低、分辨率低、需要大量样品、仪器所需的投资大	[6-7]
¹H-NMR	预处理简单、无偏向、范围广、无创性	样品用量少可回收、分析时间短、准确度高、重复性好、易制备	灵敏度低、峰信号重叠	[8，21]
¹³C-NMR	预处理要求低、一些含有矿物质的样品需要做脱矿处理	可获得结构上的细微变化	容易产生干扰峰	[10，22]

方法	取样特点	优点	缺点	参考文献
GC-MS	样品需要衍生化来生成挥发性化合物	灵敏度高，有可供参考、比较的标准谱图库，可以用于代谢产物定性	对样品有破坏性，进样需前处理增大误差，预处理过程繁杂	[6，23]
LC-MS	样品预处理复杂	适合分析复杂混合体系，高通量、高灵敏度、较宽的动态范围，避免了GC-MS中繁杂的样品前处理	对样品有破坏性，与GC-MS比较：标准谱图库较少、定性较差、有机物识别方面比较差	[6，24]
FT-ICR MS	样品预处理简单	高分辨率、高质量精度，可进行多级质谱分析	扫描速度慢、成本较高	[19，20]
MALDI FTICR MS	样品预处理简单	分析速度快、灵敏度高、质量精度高，可提供精确的分子质量信息	损害MS谱的质量，降低目标化合物的响应，降低了MALDI定量分析的准确性和重现性	[25-26]

方法	取样特点	优点	缺点	参考文献
GC-MS	样品需要衍生化来生成挥发性化合物	灵敏度高，有可供参考、比较的标准谱图库，可以用于代谢产物定性	对样品有破坏性，进样需前处理增大误差，预处理过程繁杂	[6，23]
LC-MS	样品预处理复杂	适合分析复杂混合体系，高通量、高灵敏度、较宽的动态范围，避免了GC-MS中繁杂的样品前处理	对样品有破坏性，与GC-MS比较：标准谱图库较少、定性较差、有机物识别方面比较差	[6，24]
FT-ICR MS	样品预处理简单	高分辨率、高质量精度，可进行多级质谱分析	扫描速度慢、成本较高	[19，20]
MALDI FTICR MS	样品预处理简单	分析速度快、灵敏度高、质量精度高，可提供精确的分子质量信息	损害MS谱的质量，降低目标化合物的响应，降低了MALDI定量分析的准确性和重现性	[25-26]

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Metabolomics and its Application in Environmental Toxicology

代谢组学及其在环境毒理学中的应用

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