Abstract:

To evaluate whether hydrogen-rich saline could relieve psoriasis in imiquimod-induced mouse， the male BALB/C mice which were aged eight to twelve weeks， were intraperitoneal injected by hydrogen-rich saline and normal saline for 7 days， then 5% imiquimod cream（IMQ） was given on the back， and the intraperitoneal injection of hydrogen-rich saline or normal saline was continued for 7 days. The disease clinical parameters were rated independently for the animals using the modified target lesion psoriasis severity score. The skin sections， stained with hematoxylin-eosin， were scored on Baker score. Inflammatory cytokine levels were measured through quantitative RT-PCR and ELISA kits. Malondialdehyde （MDA） kit and paraoxonase-1（PON-1） activity was used to determin plasma oxidant. The results showed that the psoriasis mouse model was successfully induced，and the hydrogen-rich saline group had lower clinical and histological scores than the normal saline mice. In the hydrogen-rich saline group， these clinical and pathology were significantly less than the normal saline group. Moreover， in comparison with normal saline mice， the level of plasma interleukin-1 （IL-1）， IL-6 and IL-17A was lower within hydrogen-rich mice. The levels of IL-1， IL-6 and IL-17A and tumor necrosis factor -α （TNF-α） in skin of hydrogen-rich saline group was less than those in normal saline group. IL-1， IL-6 and IL-17A mRNA levels were less in hydrogen-rich mice compared with normal saline group. The level of MDA in hydrogen group were lower than those in normal saline group. Moreover， the activity of PON-1 in hydrogen group were higher than those in normal saline group. This results showed that hydrogen-rich saline could alleviate the pathological features of psoriasis in imiquimod-induced mouse model， it may be associated with reducing the level of the oxidative stress and inflammation.

Key words: psoriasis, hydrogen-rich saline, inflammation, oxidative stress

摘要：

为了探讨富氢盐水是否可以减轻咪喹莫特诱导小鼠银屑病的发生发展及其作用机制，通过对8~12周龄的雄性BALB/C小鼠腹腔注射富氢盐水和生理盐水7 d后，背部给予5%的咪喹莫特乳膏（imiquimod cream,IMQ）的同时，继续给予富氢盐水或者生理盐水腹腔注射7 d。采用改良的银屑病皮损严重程度评分对银屑病模型小鼠的疾病临床参数进行独立评分。皮肤组织切片用苏木精-伊红染色，并利用Baker进行评分。应用ELISA试剂盒以及定量RT-PCR检测炎症细胞因子的表达水平。通过丙二醛（malondialdehyde，MDA）试剂盒以及对氧磷酶-1（paraoxonase-1，PON-1）活性进行血浆氧化指标的测定。结果显示，生理盐水组和富氢盐水组咪喹莫特诱导的小鼠银屑病模型临床和组织学改变均符合银屑病的表现，同时富氢盐水组的这些表现较生理盐水组均明显减轻（P<0.05）；与生理盐水组小鼠相比，富氢盐水组小鼠的组织中炎症因子白细胞介素-1（interleukin-1, IL-1）、IL-6、IL-17A水平以及血浆中IL-1、IL-6、IL-17A和肿瘤坏死因子-α（TNF-α）水平均明显降低（P<0.05）；富氢盐水组小鼠的组织中IL-1、IL-6、IL-17 mRNA水平均低于生理盐水组；富氢盐水组MDA水平低于生理盐水组，而PON-1活性高于生理盐水组。研究结果表明，在咪喹莫特诱导的银屑病小鼠模型中，富氢盐水可以减轻小鼠银屑病的病理表现，可能与平衡机体氧化还原环境、降低炎症水平有关。

关键词: 银屑病, 富氢盐水, 炎症, 氧化应激