Purity Analysis of Bitter Gourd Hybrid Seeds by RAPD and SRAP Molecular Marker Technology

doi:10.3969/j.issn.2095-2341.2014.04.10

Curr. Biotech. ›› 2014, Vol. 4 ›› Issue (4): 286-291.DOI: 10.3969/j.issn.2095-2341.2014.04.10

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Purity Analysis of Bitter Gourd Hybrid Seeds by RAPD and SRAP Molecular Marker Technology

ZHANG Shao-ping, ZHANG Yu-can, ZHANG Wei-guang, LAI Zheng-feng, ZHENG Yun-yun, CHEN Yu-shui

1.Fujian-Taiwan Horticultural Research Center, Fujian Academy of Agricultural Sciences, Fujian Zhangzhou 363005, China;

2.Institute of Agriculture and Biological Resources, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China;

3.Fujian Academy of Agricultural Sciences, Fuzhou 350003, China

Received:2014-06-03 Online:2014-07-25 Published:2014-06-10

RAPD及SRAP两种分子标记技术对苦瓜杂交种纯度的鉴定分析

张少平1,张玉灿1,2*,张伟光3,赖正锋1,郑云云1,陈玉水1

1.福建省农业科学院闽台园艺研究中心, 福建漳州 363005;

2.福建省农业科学院农业生物资源研究所, 福州 350003;

3.福建省农业科学院, 福州 350003

通讯作者: 张玉灿，研究员，主要从事苦瓜等瓜类作物相关育种研究。E-mail：996328193@qq.com
作者简介:张少平，农艺师，主要从事苦瓜等药食同源蔬菜相关研究。E-mail：zspnc@163.com。
基金资助:
福建省农业科学院科技创新团队—瓜类育种科技创新团队项目(CXTD-1-04)资助。

Abstract

Abstract: Using RAPD and SRAP molecular marker technology, the genomic DNA of Ruyu No.11 bitter gourd and its parents (or female) were analyzed to obtain differential gene fragments. After repeated experiments of DNA extraction, PCR amplification and analysis by agarose gel electrophoresis of PCR products, in the tested 46 RAPD primers and 121 pairs of SRAP primers, only 1 RAPD primer and 1 SRAP primer pair could amplify the characteristic polymorphic bands to distinguish between F1 generation hybrid and the female parent. Further test showed that the two primers from RAPD and SRAP, which possessed high stability and repeatability, could be utilized in purity identification of Ruyu No.11 seed.

Key words: bitter gourd, RAPD, SRAP, hybrid, purity

摘要： 以F1代苦瓜杂交种如玉11号及其亲本为材料，利用RAPD及SRAP两种分子标记技术对这3种苦瓜基因组DNA进行比较分析，以获得该杂交种及其亲本（或母本）差异目的基因片段。经过多次对该3种苦瓜叶片DNA提取，PCR扩增及其PCR产物的琼脂糖凝胶电泳分析，在供试的46个RAPD引物及121对SRAP引物中，筛选出1个RAPD引物及1对SRAP引物能区分该苦瓜杂交种及其母本种子，通过进一步验证分析，证明该两种分子标记的特异引物可作为如玉11号苦瓜杂交种子的纯度鉴定之用。

关键词: 苦瓜, RAPD, SRAP, 杂交种, 纯度

ZHANG Shao-ping1, ZHANG Yu-can1,2*, ZHANG Wei-guang3, LAI Zheng-feng1, ZHENG Yun-yun1, CHEN Yu-shui1. Purity Analysis of Bitter Gourd Hybrid Seeds by RAPD and SRAP Molecular Marker Technology[J]. Curr. Biotech., 2014, 4(4): 286-291.

张少平,张玉灿,张伟光,赖正锋,郑云云,陈玉水. RAPD及SRAP两种分子标记技术对苦瓜杂交种纯度的鉴定分析[J]. 生物技术进展, 2014, 4(4): 286-291.

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Purity Analysis of Bitter Gourd Hybrid Seeds by RAPD and SRAP Molecular Marker Technology

RAPD及SRAP两种分子标记技术对苦瓜杂交种纯度的鉴定分析

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