Waterlogging Tolerance of vgb Transgenic Brassica napus

doi:10.3969/j.issn.2095-2341.2014.04.09

Curr. Biotech. ›› 2014, Vol. 4 ›› Issue (4): 280-285.DOI: 10.3969/j.issn.2095-2341.2014.04.09

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Waterlogging Tolerance of vgb Transgenic Brassica napus

LI Pei-yu, ZHANG Rui, SUN Guo-qing, MA Lin, XUE Ji-xiong, LIN Qin, GUO San-dui

1.National Key Facility of Crop Gene Resources and Genetic Improvement, Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China;

2.College of Agriculture, Xinjiang Agricultural University, Urumqi 830052, China

Received:2014-05-23 Online:2014-07-25 Published:2014-07-06

转vgb基因油菜的耐涝性

李培玉1,2,张锐1,孙国清1,马林2,薛计雄1,2,林芹1,2,郭三堆1

1.中国农业科学院生物技术研究所, 农作物基因资源与基因改良国家重大科学工程, 北京 100081;

2.新疆农业大学农学院, 乌鲁木齐 830052

通讯作者: 郭三堆，研究员，博士生导师，主要从事棉花基因工程研究。E-mail:gsdui@cass.cn
作者简介:李培玉，硕士研究生，研究方向为基因工程。E-mail:lpy-0000@163.com。
基金资助:
国家转基因生物新品种培育重大专项(2011ZX08005-004)资助。

Abstract

Abstract: Rape is an important oilseed and protein crop. Waterlogging affects the development of rape in China. The improvement of rape waterlogging tolerance ability is of great significance for the sustainable development of rape. The vgb transgenic plants were identified by PCR and Southern blot. The change of SOD，MDA and proline contents in transgenic plants were significantly lower than those in the control after 15 d waterlogging treatment. Agronomic traits of plants were investigated after waterlogging，the result showed that waterlogging tolerance of vgb transgenic plants were significantly enhanced compared with that of control， indicating that expression of vgb gene in Brassica napus have a significant effect on its waterlogging tolerance.

Key words: transgenic, Brassica napus, vgb, waterlogging tolerance

摘要： 油菜是我国重要的油料作物和蛋白质饲料作物，涝害严重影响了我国油菜产业的发展，提高油菜的耐涝能力对于我国油菜可持续发展具有非常重大的意义。本研究采用PCR、Southern杂交等方法对转vgb基因油菜植株进行鉴定，15 d淹涝实验结果显示，转基因油菜的超氧化物歧化酶、丙二醛和脯氨酸含量在淹涝前后的变幅显著低于对照。对淹水后的农艺性状进行调查，转vgb基因的油菜相比较对照抗涝性明显得到增强，证明vgb基因在油菜中的表达对油菜的抗涝性具有显著作用。

关键词: 转基因, 油菜, 透明颤菌血红蛋白基因, 耐涝

LI Pei-yu1,2, ZHANG Rui1, SUN Guo-qing1, MA Lin1,2, XUE Ji-xiong1,2, LIN Qin1,2, GUO San-dui1. Waterlogging Tolerance of vgb Transgenic Brassica napus[J]. Curr. Biotech., 2014, 4(4): 280-285.

李培玉,张锐,孙国清,马林,薛计雄, ,林芹,郭三堆. 转vgb基因油菜的耐涝性[J]. 生物技术进展, 2014, 4(4): 280-285.

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Waterlogging Tolerance of vgb Transgenic Brassica napus

转vgb基因油菜的耐涝性

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