Abstract: Sericin1 gene (Ser1) of silkworm (Bombyx mori) is specifically expressed in middle silkgland, its promoter plays an important role in silkworm bioreactor. In order to learn polymorphisms of upstream regulation sequence of Ser1 gene from  silkworm breed Yun 7A, we obtained Ser1 promoter sequences that had driver activity, Ser1 promoter sequence was cloned and then analyzed, the expression vector of pBac [Ser1p-DsRed-SV40+A3-EGFP] contained DsRed (red fluorescent protein gene)was driven by Ser1 promoter, the vector was transferred to BmN cells to verify the promoter activity through transient transfection expression assay. Sequence alignment showed that cis element area had been highly conservative between upstream regulation sequence of reported Ser1 and Ser1 promoter sequence of Yun 7A, the other area showed absence of the genetic phenomenon of 422 bp bases; there were conservative sequences of TATA box and CAAT box located at position -24～-30 and -112～-115 respectively, the sequences of TATA box was TATAAAA; the promoter of Ser1 could drive DsRed gene, the transiently expression products could be observed successfully in the cultural BmN cells and middle silkgland. The polymorphism and driver activity of Ser1 upstream regulation sequence from Yunnan silkworm breed were analyzed in this study, so as to lay the foundation for understanding the ability difference of sericin synthetic in the different silkworm breeds and acquiring the efficient promoter.

Key words: Bombyx mori, Ser1 gene, promoter, activity analysis

摘要： 家蚕Sericin1基因（Ser1）是中部丝腺特异表达基因，其启动子在家蚕生物反应器研究方面发挥着重要作用。为了解云南家蚕品种云7 Ser1基因上游调控序列存在的多态性，进而获得具有驱动活性的Ser1启动子序列，克隆了家蚕Ser1基因启动子并进行序列分析，构建了由该基因启动子驱动红色荧光蛋白基因DsRed的表达载体pBac [Ser1p-DsRed-SV40+A3-EGFP]，转染家蚕BmN细胞进行瞬时表达来验证启动子活性。该启动子同已报道Ser1基因上游调控序列比对发现，顺式作用元件区域高度保守，而其他区域存在422 bp的碱基缺失；Ser1基因上游调控序列中存在启动子TATA框和CAAT框分别位于-24～-30处和-112～-115处，其中TATA框的保守序列是TATAAAA；而启动子驱动下的DsRed基因在BmN细胞和中部丝腺中成功表达。结果表明云南家蚕品种Ser1上游调控序列存在多态性和驱动活性，为了解不同家蚕品种丝胶合成能力差异和获得高效启动子奠定了基础。

关键词: 家蚕, Ser1基因, 启动子, 活性分析