Abstract: Promoter is an important element in regulation of gene expression. The regulation role of promoter is the result of a multi-level interaction of multiple factors. The level, location and manner of gene expression are regulated by the promoter, so deeply study of promoter is of great importance for understanding biological growth, defense system and disease. This paper reviews the methods of promoter cloning, bioinformatics analysis and forecasting of promoter, compares two methods of promoter analysis, introduces the progress of promoter methylation and polymorphism, so as to provide a reference for further study of promoters.

Key words: promoter, clone, bioinformatics, transcription factor, methylation, polymorphism

摘要： 启动子是调控基因表达的重要基因元件，它的调控作用是多层次多因素共同作用的结果。通过启动子的调控能够控制基因表达的水平、部位及方式。深入研究启动子对于了解生物的生长发育、防御系统、疾病等都有非常重要的意义。本文综述了启动子克隆、生物信息学分析和预测的方法，比较了两种启动子分析方法，介绍了启动子甲基化、多态性和合成启动子的研究进展，以期能够为启动子的研究提供参考。

关键词: 启动子, 克隆, 生物信息学, 转录因子, 甲基化, 多态性