Analysis of Clinical Application and Technology Hotspots of Digital PCR Technology Based on Bibliometrics Methods

doi:10.19586/j.2095-2341.2021.0184

Current Biotechnology ›› 2022, Vol. 12 ›› Issue (4): 606-613.DOI: 10.19586/j.2095-2341.2021.0184

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Analysis of Clinical Application and Technology Hotspots of Digital PCR Technology Based on Bibliometrics Methods

Peixuan MEN(), Yufeng XIAO, Bin ZHANG()

Institute of Medical Information/Medical Library，Chinese Academy of Medical Sciences and Peking Union Medical College，Beijing 100005，China

Received:2021-11-16 Accepted:2021-12-21 Online:2022-07-25 Published:2022-08-10
Contact: Bin ZHANG

基于计量学方法分析数字PCR技术的临床应用现状与技术热点

门佩璇(), 肖宇锋, 张玢()

中国医学科学院医学信息研究所/图书馆，北京 100005

通讯作者: 张玢
作者简介:门佩璇 E-mail：men.peixuan@imicams.ac.cn；
基金资助:
NSTL生命健康安全国际战略与政策情报研究专项(2021XM59-8)

Abstract

Abstract:

As the third generation PCR technology， digital PCR has developed rapidly in recent years and has been applied in many fields such as non-invasive prenatal screening， viral nucleic acid detection and tumor liquid biopsy. Chinese and English papers and patent documents related to “clinical application of digital PCR technology” included in the Chinese Biomedical Literature Database， PubMed Medical Literature Retrieval Service System， and Incopat Patent Database in this study as the data sources. The paper analyzed the current clinical application and technology hotpot trends of digital PCR according to bibliometrics methods， and clarified the main research organization， content and direction of this technology. The main institutions of digital PCR include Fred Hutchinson Cancer Research Center， Dana-farber Cancer Institute， Harvard Medical School， and Biorad inc. Chinese articles published by inspection and quarantine institutions accounted for a large proportion. At present， the research on digital PCR mainly focused on tumor concomitant diagnosis， virus detection and gene expression analysis. China has high enthusiasm for digital PCR research， especially in patent application， it has shown a leading trend.

Key words: bibliometrics methods, digital PCR, clinical application, research situation, technology hotspots

摘要：

近年来数字PCR技术作为第三代PCR技术迅猛发展，已被应用于无创产前筛查、病毒核酸检测及肿瘤液体活检等领域。以中国生物医学文献数据库、PubMed医学文献检索服务系统及Incopat专利数据库收录的与“数字PCR技术临床应用”相关的中英文论文和专利文献为数据源，利用计量学方法分析了数字PCR临床应用现状与技术热点趋势，明确该技术的主要研究机构包括美国弗雷德-哈钦森肿瘤研究中心、哈佛医学院丹娜法伯癌症研究所和Bio-Rad公司等。中文文献发表机构中检验检疫部门和疾控中心占比较高。国内外数字PCR技术研究主要聚焦于肿瘤伴随诊断、病毒检测和基因表达分析等方面。中国数字PCR技术研究热情较高，专利申请已展现出国际领先趋势。

关键词: 计量学方法, 数字PCR, 临床应用, 研究现状, 技术热点

CLC Number:

Q503

Peixuan MEN, Yufeng XIAO, Bin ZHANG. Analysis of Clinical Application and Technology Hotspots of Digital PCR Technology Based on Bibliometrics Methods[J]. Current Biotechnology, 2022, 12(4): 606-613.

门佩璇, 肖宇锋, 张玢. 基于计量学方法分析数字PCR技术的临床应用现状与技术热点[J]. 生物技术进展, 2022, 12(4): 606-613.

Figures/Tables 10

References 27

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检测对象	临床应用
循环肿瘤ctDNA	非侵入性检测非小细胞肺癌、前列腺癌、乳腺癌、头颈部鳞状细胞癌的基因突变
	肿瘤标本的等位基因绝对定量、罕见突变检测、拷贝数变异分析、DNA甲基化和基因重排等
	指导肺腺癌EGFR⁃TKI个性化治疗
胎儿游离DNA	诊断胎儿染色体非整体疾病
胎儿游离DNA	检测甲基丙二酸血症、血友病突变基因等单基因遗传病
病原微生物核酸	检测HIV、新冠肺炎病毒等病原体鉴定
	检测HBV、结核分枝杆菌等耐药突变
	检测青光眼睫状体炎综合征患者房水的人巨细胞病毒
供体来源游离DNA	非侵入性检测移植后的排斥反应

检测对象	临床应用
循环肿瘤ctDNA	非侵入性检测非小细胞肺癌、前列腺癌、乳腺癌、头颈部鳞状细胞癌的基因突变
	肿瘤标本的等位基因绝对定量、罕见突变检测、拷贝数变异分析、DNA甲基化和基因重排等
	指导肺腺癌EGFR⁃TKI个性化治疗
胎儿游离DNA	诊断胎儿染色体非整体疾病
胎儿游离DNA	检测甲基丙二酸血症、血友病突变基因等单基因遗传病
病原微生物核酸	检测HIV、新冠肺炎病毒等病原体鉴定
	检测HBV、结核分枝杆菌等耐药突变
	检测青光眼睫状体炎综合征患者房水的人巨细胞病毒
供体来源游离DNA	非侵入性检测移植后的排斥反应

序号	机构名称	所属国家	文献数
1	弗雷德-哈钦森肿瘤研究中心	美国	31
2	哈佛医学院丹娜法伯癌症研究所	美国	25
3	Bio⁃Rad公司	美国	18
3	清华大学	中国	17
4	奥胡斯大学	丹麦	17
5	九州大学	日本	14
6	法国国家健康与医学研究院	法国	8
7	近畿大学	日本	7
8	筑波大学	日本	7
9	布朗大学阿尔伯特医学院	美国	7

序号	机构名称	所属国家	文献数
1	弗雷德-哈钦森肿瘤研究中心	美国	31
2	哈佛医学院丹娜法伯癌症研究所	美国	25
3	Bio⁃Rad公司	美国	18
3	清华大学	中国	17
4	奥胡斯大学	丹麦	17
5	九州大学	日本	14
6	法国国家健康与医学研究院	法国	8
7	近畿大学	日本	7
8	筑波大学	日本	7
9	布朗大学阿尔伯特医学院	美国	7

序号	申请人	专利数量
1	领航基因科技（杭州）有限公司	21
2	宁波胤瑞生物医学仪器有限责任公司	12
3	广东出入境检验检疫局检验检疫技术中心	12
4	新羿制造科技（北京）有限公司	12
5	上海赛安生物医药科技股份有限公司	11
6	中国检验检疫科学研究院	11
7	上海新微技术研发中心有限公司	10
8	北京致雨生物科技有限公司	10
9	中国科学院苏州生物医学工程技术研究所	8
10	Bio⁃Rad生命医学产品公司	7
11	加州理工学院	7
12	利兰斯坦福初级大学董事	7
13	中山出入境检验检疫局检验检疫技术中心	7
14	中山大学达安基因股份有限公司	7
15	北京爱普拜生物技术有限公司	7
16	暨南大学	7
17	美国生命技术公司	6
18	中国科学院上海微系统与信息技术研究所	6
19	浙江省农业科学院	6
20	良培基因生物科技（武汉）有限公司	6

Analysis of Clinical Application and Technology Hotspots of Digital PCR Technology Based on Bibliometrics Methods

基于计量学方法分析数字PCR技术的临床应用现状与技术热点

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