N-PS对皮肤屏障损伤预防与修复的效果评价

doi:10.19586/j.2095-2341.2025.0160

生物技术进展 ›› 2026, Vol. 16 ›› Issue (2): 432-438.DOI: 10.19586/j.2095-2341.2025.0160

• 研究论文 • 上一篇

N-PS对皮肤屏障损伤预防与修复的效果评价

程贤(), 张祝兰(), 陈洲琴, 高晓晶, 杨煌建, 严凌斌, 连云阳()

福建省微生物研究所，福建省新药（微生物）筛选重点实验室，福州 350007

收稿日期:2025-11-17 接受日期:2026-01-05 出版日期:2026-03-25 发布日期:2026-04-27
通讯作者: 张祝兰,连云阳
作者简介:程贤 E-mail: 331788272@qq.com
基金资助:
福建省属公益类研究所基本科研专项(2022R1005007);福建省属公益类研究所基本科研专项(2025R1005006)

Evaluation of the Preventive and Reparative Effects of N-PS on Skin Barrier Impairment

Xian CHENG(), Zhulan ZHANG(), Zhouqin CHEN, Xiaojing GAO, Huangjian YANG, Lingbin YAN, Yunyang LIAN()

Fujian Provincial Key Laboratory of Screening for Novel Microbial Products，Fujian Institute of Microbiology，Fuzhou 350007，China

Received:2025-11-17 Accepted:2026-01-05 Online:2026-03-25 Published:2026-04-27
Contact: Zhulan ZHANG,Yunyang LIAN

摘要/Abstract

摘要：

研究旨在探讨红色诺卡氏菌细胞壁骨架多糖组分（Nocardia rubra cell wall skeleton polysaccharide components, N-PS）对皮肤屏障损伤预防与修复的效果。考察N-PS的抗氧化活性及其对RAW 264.7细胞的吞噬活性、B16小鼠黑色素瘤细胞合成黑色素、酪氨酸酶活性的影响以及对H₂O₂损伤后HaCaT细胞的修复效果。在0.25~4.00 mg·mL^-1浓度范围内，N-PS具有较强的DPPH自由基清除能力，清除率最高可达40.0%。200 μg·mL^-1 N-PS刺激RAW 264.7细胞，可使细胞增殖率增加2.56%，RAW264.7细胞的吞噬活性提高26.94%，巨噬细胞介导的先天免疫反应提高65.88%；使B16小鼠黑色素瘤细胞繁殖率降低36.89%，对黑色素细胞合成黑色素的抑制率为39.05%，细胞酪氨酸酶的总活性降低38.86%。N-PS对H₂O₂损伤HaCaT细胞的保护作用较好，用800 μg·mL^-1 N-PS保护后的细胞增殖率由空白对照组的65.62%提高至97.79%。在0.25~4.00 mg·mL^-1浓度范围内，N-PS具有最高40.0%的抗氧化活性，200 μg·mL^-1的N-PS对皮肤屏障损伤有较好的预防与修复效果。

关键词: 红色诺卡氏菌, N-PS, 免疫调节, 抗氧化活性, RAW 264.7细胞, 鼠黑色素瘤B16, 皮肤屏障修复

Abstract:

This study aimed to investigate the preventive and reparative effects of Nocardia rubra cell wall skeleton polysaccharide components （N-PS） on skin barrier impairment. The antioxidant activity of N-PS was evaluated， together with its effects on phagocytic activity of RAW 264.7 cells， melanin synthesis and tyrosinase activity in B16 mouse melanoma cells， as well as the reparative effect on HaCaT cells injured by H₂O₂. Within the concentration range of 0.25~4.00 mg·mL^-1， N-PS showed strong DPPH free radical scavenging activity， with a maximum scavenging rate of 40.0%. Stimulation with 200 μg·mL^-1 N-PS increased the proliferation rate of RAW 264.7 cells by 2.56%， enhanced the phagocytic activity by 26.94%， and elevated the macrophage-mediated innate immune response by 65.88%. Meanwhile， N-PS reduced the proliferation of B16 mouse melanoma cells by 36.89%， inhibited melanin synthesis by 39.05%， and decreased total cellular tyrosinase activity by 38.86%. N-PS exhibited significant protective effects on H₂O₂-damaged HaCaT cells， and the cell viability increased from 65.62% in the blank control group to 97.79% after pretreatment with 800 μg·mL^-1 N-PS. In summary， N-PS showed a maximum DPPH scavenging activity of 40.0% at 0.25~4.00 mg·mL^-1， and 200 μg·mL^-1 N-PS exerted favorable preventive and reparative effects on skin barrier impairment.

Key words: Nocardia rubra, N-PS, immune regulation, antioxidant activity, RAW 264.7 cells, mouse melanoma B16, skin barrier repair

中图分类号:

Q819

程贤, 张祝兰, 陈洲琴, 高晓晶, 杨煌建, 严凌斌, 连云阳. N-PS对皮肤屏障损伤预防与修复的效果评价[J]. 生物技术进展, 2026, 16(2): 432-438.

Xian CHENG, Zhulan ZHANG, Zhouqin CHEN, Xiaojing GAO, Huangjian YANG, Lingbin YAN, Yunyang LIAN. Evaluation of the Preventive and Reparative Effects of N-PS on Skin Barrier Impairment[J]. Current Biotechnology, 2026, 16(2): 432-438.

图/表 5

图1 不同浓度N-PS的DPPH自由基清除活性注：**表示与对照组相比，实验组在P<0.01水平上具有统计学意义。

Fig. 1 DPPH radical scavenging activity of N-PS at different concentrations

图2 N-PS对RAW 264.7细胞的影响A：N-PS对RAW 264.7细胞吞噬中性红的影响；B：N-PS对RAW 264.7细胞增殖率的影响；C：N-PS对RAW 264.7细胞NO含量的影响。*、**表示与对照组相比，实验组在P<0.05和P<0.01水平上具有统计学意义。

Fig. 2 The effect of N-PS on the RAW 264.7 cells

图3 N-PS对B16小鼠黑色素瘤细胞的影响A：N-PS对B16小鼠黑色素瘤细胞增殖率的影响；B：N-PS对B16小鼠黑色素瘤细胞黑色素合成总量的影响；C：N-PS对B16小鼠黑色素瘤细胞酪氨酸酶活性的影响。*、**表示与对照组相比，实验组在P<0.05和P<0.01水平上具有统计学意义。

Fig. 3 The effect of N-PS on the B16 mouse melanoma cells

图4 不同浓度H2O2和N-PS对HaCaT细胞增殖率的影响A:不同浓度H2O2对HaCaT细胞增殖率的影响；B:不同浓度N-PS对HaCaT细胞增殖率的影响。*、**表示与对照组相比，实验组在P<0.05、P<0.01水平上具有统计学意义。

Fig. 4 The effect of different concentrations of H2O2 and N-PS on the proliferation rate of HaCaT cells

图5 N-PS对H2O2损伤HaCaT细胞的影响注：*、**表示与对照组相比，实验组在P<0.05、P<0.01水平上具有统计学意义。

Fig. 5 The effect of N-PS on H2O2 induced damage to HaCaT cells

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N-PS对皮肤屏障损伤预防与修复的效果评价

Evaluation of the Preventive and Reparative Effects of N-PS on Skin Barrier Impairment

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