植物乳植杆菌HCS03-001冻干粉制备工艺优化及抗幽门螺杆菌的功效

doi:10.19586/j.2095-2341.2023.0125

生物技术进展 ›› 2024, Vol. 14 ›› Issue (2): 287-294.DOI: 10.19586/j.2095-2341.2023.0125

植物乳植杆菌HCS03-001冻干粉制备工艺优化及抗幽门螺杆菌的功效

汪海涛¹^,²(), 宋佳¹^,², 余萍¹^,²(), 陈雪娇¹^,²

^1.江西仁仁健康微生态科技有限公司，江西樟树 331200
^2.仁仁微生物科技研究（沈阳）有限公司，沈阳 110170

收稿日期:2023-10-17 接受日期:2024-02-04 出版日期:2024-03-25 发布日期:2024-04-17
通讯作者: 余萍
作者简介:汪海涛 E-mail: 724312941@qq.com；

Optimization of Preparation Technology of Lactiplantibacillus plantarum HCS03-001 Freeze-dried Powder and its Anti-Helicobacter pylori Function

Haitao WANG¹^,²(), Jia SONG¹^,², Ping YU¹^,²(), Xuejiao CHEN¹^,²

^1.Jiangxi Renren Health Microecological Technology Co. ，Ltd. ，Jiangxi Zhangshu 331200，China
^2.Renren Microbial Technology Research （Shenyang） Co. ，Ltd. ，Shenyang 110170，China

Received:2023-10-17 Accepted:2024-02-04 Online:2024-03-25 Published:2024-04-17
Contact: Ping YU

摘要/Abstract

摘要：

为优化植物乳植杆菌（Lactiplantibacillus plantarum）HCS03-001的冻干粉制备工艺，探究其抗幽门螺杆菌的功能效果，通过单因素试验、主因素分析和响应面试验优化植物乳植杆菌HCS03-001冻干粉的制备工艺，采用体外抑菌试验验证该益生菌的抗幽门螺杆菌功效。结果发现，优化后制备冻干粉的最佳工艺条件为：发酵温度37 ℃，发酵时间16 h，接种量5.2%，冻干时间44 h，在此条件下活菌数达到5.85×10¹¹ CFU·g^-1。体外抑菌试验结果表明植物乳植杆菌HCS03-001的发酵上清液和菌悬液均能有效抑制幽门螺杆菌的生长，抑制率达到69.55%~78.78%。研究结果表明植物乳植杆菌HCS03-001具备抗幽门螺杆菌的功能。

关键词: 植物乳植杆菌, 冻干粉, 响应面, 幽门螺杆菌

Abstract:

The aim of the study is to optimize the preparation technology of freeze-dried powder of Lactiplantibacillus plantarum HCS03-001 and explore its functional effect of anti-Helicobacter pylori. In this study， the preparation technology of freeze-dried powder of Lactiplantibacillus plantarum HCS03-001 was optimized by single factor test， principal factor analysis and response surface test， and then the anti-Helicobacter pylori effect of the probiotics was verified by bacteriostatic test in vitro. The results showed that the optimum conditions for preparing freeze-dried powder were fermentation temperature 37 ℃， fermentation time 16 h， inoculum 5.2% and freeze-drying time 44 h. Under these conditions， the number of live bacteria was 5.85 × 10¹¹ CFU·g^-1. These results of bacteriostatic test in vitro showed that the fermentation supernatant and bacterial suspension of Lactiplantibacillus plantarum HCS03-001 could effectively inhibit the growth of Helicobacter pylori， and the inhibition rate was 69.55%~78.78%， which showed that Lactiplantibacillus plantarum HCS03-001 have the function of anti-Helicobacter pylori.

Key words: Lactiplantibacillus plantarum, freeze-dried powder, response surface, Helicobacter pylori

中图分类号:

Q815

汪海涛, 宋佳, 余萍, 陈雪娇. 植物乳植杆菌HCS03-001冻干粉制备工艺优化及抗幽门螺杆菌的功效[J]. 生物技术进展, 2024, 14(2): 287-294.

Haitao WANG, Jia SONG, Ping YU, Xuejiao CHEN. Optimization of Preparation Technology of Lactiplantibacillus plantarum HCS03-001 Freeze-dried Powder and its Anti-Helicobacter pylori Function[J]. Current Biotechnology, 2024, 14(2): 287-294.

图/表 10

参考文献 20

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因素	水平
因素	-1	1
发酵温度/℃	35	36
发酵时间/h	8	12
初始pH	6.0	6.3
接种量/%	1	3
冻干保护剂添加比例	1∶1	1:2
冻干时间/h	40	42

因素	水平
因素	-1	1
发酵温度/℃	35	36
发酵时间/h	8	12
初始pH	6.0	6.3
接种量/%	1	3
冻干保护剂添加比例	1∶1	1:2
冻干时间/h	40	42

项目	平方和	自由度	均方	F值	P值
模型	6.73	14	0.48	1 344.61	<0.000 1
A-发酵温度	0.31	1	0.31	859.3	<0.000 1
B-发酵时间	0.15	1	0.15	412.33	<0.000 1
C-接种量	8.01×10^-3	1	8.01×10^-4	22.4	0.000 3
D-冻干时间	0.11	1	0.11	313.66	<0.000 1
AB	4.23×10^-3	1	4.23×10^-4	11.82	0.004
AC	1.23×10^-3	1	1.23×10^-4	3.43	0.085 4
AD	1.00×10^-4	1	1.00×10^-4	0.28	0.605 2
BC	0	1	0	0	1
BD	1.00×10^-4	1	1.00×10^-4	0.28	0.605 2
CD	0	1	0	0	1
A²	1.79	1	1.79	5 020.01	<0.000 1
B²	0.25	1	0.25	688.16	<0.000 1
C²	0.032	1	0.032	88.27	<0.000 1
D²	5.12	1	5.12	14 323.47	<0.000 1
残差	5.01×10^-3	14	3.58×10^-4	—	—
失拟项	3.13×10^-3	10	3.13×10^-4	0.66	0.726 9
纯误差	1.88×10^-3	4	4.70×10^-4	—	—
总离差	6.73	28	—	—	—

项目	平方和	自由度	均方	F值	P值
模型	6.73	14	0.48	1 344.61	<0.000 1
A-发酵温度	0.31	1	0.31	859.3	<0.000 1
B-发酵时间	0.15	1	0.15	412.33	<0.000 1
C-接种量	8.01×10^-3	1	8.01×10^-4	22.4	0.000 3
D-冻干时间	0.11	1	0.11	313.66	<0.000 1
AB	4.23×10^-3	1	4.23×10^-4	11.82	0.004
AC	1.23×10^-3	1	1.23×10^-4	3.43	0.085 4
AD	1.00×10^-4	1	1.00×10^-4	0.28	0.605 2
BC	0	1	0	0	1
BD	1.00×10^-4	1	1.00×10^-4	0.28	0.605 2
CD	0	1	0	0	1
A²	1.79	1	1.79	5 020.01	<0.000 1
B²	0.25	1	0.25	688.16	<0.000 1
C²	0.032	1	0.032	88.27	<0.000 1
D²	5.12	1	5.12	14 323.47	<0.000 1
残差	5.01×10^-3	14	3.58×10^-4	—	—
失拟项	3.13×10^-3	10	3.13×10^-4	0.66	0.726 9
纯误差	1.88×10^-3	4	4.70×10^-4	—	—
总离差	6.73	28	—	—	—

组别	OD₆₀₀	抑菌圈直径/mm	幽门螺杆菌抑制率/%
空白对照	—	0	0
阳性对照	—	20.92±1.02**	100.00**
菌悬液	1.911	16.48±2.14**	78.78**
发酵上清液	0.253	14.55±0.84**	69.55**

植物乳植杆菌HCS03-001冻干粉制备工艺优化及抗幽门螺杆菌的功效

Optimization of Preparation Technology of Lactiplantibacillus plantarum HCS03-001 Freeze-dried Powder and its Anti-Helicobacter pylori Function

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参考文献 20

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实验编号	发酵温度/℃	发酵时间/h	初始pH	接种量/%	冻干保护剂	冻干时间/h	活菌数/（×10¹¹ CFU·g^-1）
1	-1	-1	-1	-1	-1	-1	5.46
2	-1	1	-1	-1	-1	1	5.31
3	1	-1	1	1	-1	1	5.18
4	1	1	1	-1	1	1	5.11
5	1	1	-1	1	1	-1	5.06
6	-1	-1	-1	1	1	1	4.87
7	1	1	-1	1	-1	-1	5.25
8	1	-1	1	-1	-1	-1	5.29
9	-1	-1	1	1	1	-1	5.33
10	-1	1	1	-1	1	-1	5.02
11	1	-1	-1	-1	1	1	5.33
12	-1	1	1	1	-1	1	5.26

序号	A：发酵温度/℃	B：发酵时间/h	C：接种量/%	D：冻干时间/h	活菌数/（×10¹¹CFU·g^-1）
1	0	1	1	0	5.68
2	0	0	0	0	5.79
3	0	-1	1	0	5.48
4	0	-1	-1	0	5.44
5	0	0	0	0	5.81
6	0	0	-1	-1	4.74
7	0	0	-1	1	4.94
8	-1	1	0	0	5.09
9	0	-1	0	-1	4.52
10	1	-1	0	0	5.17
11	0	0	1	1	4.98
12	0	0	0	0	5.83
13	0	1	0	-1	4.75
14	1	0	0	-1	4.49
15	0	0	1	-1	4.78
16	0	1	-1	0	5.64
17	1	0	1	0	5.45
18	-1	0	1	0	5.08
19	1	1	0	0	5.33
20	1	0	-1	0	5.34
21	-1	0	0	1	4.34
22	0	0	0	0	5.84
23	0	-1	0	1	4.72
24	0	0	0	0	5.84
25	0	1	0	1	4.97
26	1	0	0	1	4.65
27	-1	0	0	-1	4.16
28	-1	-1	0	0	4.80
29	-1	0	-1	0	5.04

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