基于HPLC指纹图谱和化学计量学的蒲公英质量评价研究

doi:10.19586/j.2095-2341.2025.0110

生物技术进展 ›› 2026, Vol. 16 ›› Issue (1): 105-114.DOI: 10.19586/j.2095-2341.2025.0110

基于HPLC指纹图谱和化学计量学的蒲公英质量评价研究

李斌¹(), 周玉梅¹, 王霞¹, 宋志军², 闫炳雄²()

^1.会宁县种子管理站，甘肃会宁 730700
^2.广西壮族自治区药用植物园，西南濒危药材资源开发国家工程研究中心，南宁 530023

收稿日期:2025-08-22 接受日期:2025-10-23 出版日期:2026-01-25 发布日期:2026-02-12
通讯作者: 闫炳雄
作者简介:李斌 E-mail: 973826098@qq.com；
基金资助:
广西壮族自治区中药管理局项目(GXZYA20230003);广西药用植物园保育人才小高地自主研究项目(GXYYXGD202203);广西壮族自治区药用植物园创新团队项目(桂药创2019006)

Study on Quality Evaluation of Dandelion Based on HPLC Fingerprint and Chemometrics

Bin LI¹(), Yumei ZHOU¹, Xia WANG¹, Zhijun SONG², Bingxiong YAN²()

^1.Huining County Seed Management Station，Gansu Huining 730700，China
^2.National Engineering Research Center for Southwest Endangered Medicinal Materials Resources Development，Guangxi Botanical Garden of Medicinal Plants，Nanning 530023，China

Received:2025-08-22 Accepted:2025-10-23 Online:2026-01-25 Published:2026-02-12
Contact: Bingxiong YAN

摘要/Abstract

摘要：

为综合评价会宁地区蒲公英的质量特征，建立了高效液相色谱法（high performance liquid chromatography，HPLC）指纹图谱并进行相似度评价分析。采用化学模式识别方法对15批蒲公英进行主成分分析（principal component analysis，PCA）和正交偏最小二乘法-判别分析（orthogonal partial least squares discriminant analysis，OPLS-DA），并结合多成分含量测定，对会宁地区蒲公英的品质进行综合评价。结果表明，蒲公英指纹图谱有20个共有峰，除S3和S12外，其余13批相似度均大于0.89；PCA将15批蒲公英按来源划分为2类，OPLS-DA进一步筛选出5个差异性标志物，分别是咖啡酸、新绿原酸、阿魏酸、菊苣酸和绿原酸，且差异显著性排序为咖啡酸>新绿原酸>阿魏酸>菊苣酸>绿原酸；含量测定结果显示，蒲公英菊苣酸含量均达到药典规定标准，且栽培种植蒲公英中的咖啡酸和菊苣酸普遍高于野生蒲公英。研究建立的蒲公英质量综合评价方法能有效分析不同批次蒲公英质量的差异性，为蒲公英的质量评价、栽培种植以及健康产品开发提供科学依据。

关键词: 蒲公英, 指纹图谱, 化学计量, 含量测定, 质量评价

Abstract:

To comprehensively evaluate the quality characteristics of dandelion from Huining region， this study established an high performance liquid chromatography（HPLC） fingerprint and conducted similarity evaluation. Chemical pattern recognition methods， including principal component analysis （PCA） and orthogonal partial least squares discriminant analysis（OPLS-DA）， were applied to analyze 15 batches of dandelion samples. Additionally， multi-component content determination was performed to comprehensively assess the quality of dandelion from Huining region. The results showed that the dandelion fingerprint contained 20 common peaks， with similarity values greater than 0.89 for 13 batches except S3 and S12. PCA classified the 15 batches into two categories based on their origin and OPLS-DA further identified five differential makers， namely caffeic acid， neochlorogenic acid， ferulic acid， chicoric acid， and chlorogenic acid. The significance of differences ranked as follows： caffeic acid>neochlorogenic acid>ferulic acid>chicoric acid>chlorogenic acid. The content determination results indicated that the chicoric acid content in all dandelion samples exceeded the current pharmacopoeia standards， and the levels of caffeic acid and chicoric acid in cultivated dandelion were generally higher than those in wild dandelion. The established comprehensive quality evaluation method for dandelion effectively analyzed the differences in quality among different batches， providing a scientific basis for quality assessment， cultivation， and the development of health products derived from dandelion.

Key words: dandelion, fingerprint, chemometrics, content determination, quality evaluation

中图分类号:

Q946.81+9

李斌, 周玉梅, 王霞, 宋志军, 闫炳雄. 基于HPLC指纹图谱和化学计量学的蒲公英质量评价研究[J]. 生物技术进展, 2026, 16(1): 105-114.

Bin LI, Yumei ZHOU, Xia WANG, Zhijun SONG, Bingxiong YAN. Study on Quality Evaluation of Dandelion Based on HPLC Fingerprint and Chemometrics[J]. Current Biotechnology, 2026, 16(1): 105-114.

图/表 9

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编号	样品来源	编号	样品来源
S1	会师镇野生蒲公英	S9	郭城驿野生蒲公英
S2	会师镇野生蒲公英	S10	老君坡镇种植蒲公英
S3	杨崖集镇野生蒲公英	S11	老君坡镇种植蒲公英
S4	中川镇野生蒲公英	S12	太平店镇种植蒲公英
S5	新塬镇野生蒲公英	S13	太平店镇种植蒲公英
S6	白草塬镇野生蒲公英	S14	甘肃蒲岳农业科技开发有限公司
S7	大沟镇野生蒲公英	S15	甘肃蒲岳农业科技开发有限公司
S8	大沟镇野生蒲公英

编号	样品来源	编号	样品来源
S1	会师镇野生蒲公英	S9	郭城驿野生蒲公英
S2	会师镇野生蒲公英	S10	老君坡镇种植蒲公英
S3	杨崖集镇野生蒲公英	S11	老君坡镇种植蒲公英
S4	中川镇野生蒲公英	S12	太平店镇种植蒲公英
S5	新塬镇野生蒲公英	S13	太平店镇种植蒲公英
S6	白草塬镇野生蒲公英	S14	甘肃蒲岳农业科技开发有限公司
S7	大沟镇野生蒲公英	S15	甘肃蒲岳农业科技开发有限公司
S8	大沟镇野生蒲公英

对照品	标准曲线方程式	R²	线性范围/（mg·mL^-1）
新绿原酸	y=27 415 774x-45 572	0.999 6	0.140~1.402
绿原酸	y=27 469 882x-57 778	0.999 5	0.181~1.810
咖啡酸	y=4 698 948x-2 997	0.999 7	0.232~2.321
菊苣酸	y=39 487 351x+65 292	0.999 6	0.135~1.352
阿魏酸	y=18 989 323x-23 578	0.999 8	0.152~1.520
异绿原酸B	y=26 742 352x+47 252	0.999 5	0.119~1.190
异绿原酸A	y=10 823 104x-14 278	0.999 7	0.124~1.243
芦丁	y=2 821 032x+4 651	0.999 8	0.128~1.282
槲皮素	y=2 496 454x+1 987	0.999 8	0.145~1.450
木犀草素	y=465 648x-2 341	0.999 9	0.136~1.365
山奈酚	y=978 532x-4 657	0.999 6	0.148~1.482
芹菜素	y=1 227 312x-1 044	0.999 6	0.155~1.550

对照品	标准曲线方程式	R²	线性范围/（mg·mL^-1）
新绿原酸	y=27 415 774x-45 572	0.999 6	0.140~1.402
绿原酸	y=27 469 882x-57 778	0.999 5	0.181~1.810
咖啡酸	y=4 698 948x-2 997	0.999 7	0.232~2.321
菊苣酸	y=39 487 351x+65 292	0.999 6	0.135~1.352
阿魏酸	y=18 989 323x-23 578	0.999 8	0.152~1.520
异绿原酸B	y=26 742 352x+47 252	0.999 5	0.119~1.190
异绿原酸A	y=10 823 104x-14 278	0.999 7	0.124~1.243
芦丁	y=2 821 032x+4 651	0.999 8	0.128~1.282
槲皮素	y=2 496 454x+1 987	0.999 8	0.145~1.450
木犀草素	y=465 648x-2 341	0.999 9	0.136~1.365
山奈酚	y=978 532x-4 657	0.999 6	0.148~1.482
芹菜素	y=1 227 312x-1 044	0.999 6	0.155~1.550

编号	新绿原酸	绿原酸	咖啡酸	菊苣酸	阿魏酸	异绿原酸B	异绿原酸A	芦丁	槲皮素	木犀草素	山奈酚	芹菜素
S1	0.716	0.410	0.376	5.569	0.059	0.040	0.153	3.162	1.386	0.042	0.089	0.465
S2	0.516	0.285	0.260	4.948	0.012	0.013	0.109	6.009	4.209	0.114	0.186	0.102
S3	0.805	0.360	0.304	6.018	0.011	0.056	0.087	8.023	2.233	0.221	0.078	0.989
S4	0.911	0.238	0.407	5.778	0.038	0.084	0.011	9.106	4.325	0.192	0.059	0.322
S5	0.708	0.168	0.520	4.831	0.002	0.038	0.095	4.006	1.238	0.110	0.204	0.251
S6	0.502	0.506	0.414	4.507	0.003	0.093	0.124	5.992	3.413	0.136	0.065	0.578
S7	0.120	0.402	0.370	4.943	0.019	0.013	0.044	6.237	6.187	0.132	0.208	0.249
S8	0.094	0.660	0.654	5.529	0.005	0.013	0.308	4.851	5.279	0.118	0.185	0.423
S9	0.021	0.335	0.416	8.459	0.025	0.068	0.151	6.050	2.366	0.015	0.135	0.895
S10	0.030	0.430	0.337	6.650	0.061	0.117	0.110	4.781	5.444	0.096	0.173	0.484
S11	0.043	0.795	0.352	5.277	0.031	0.064	0.098	3.850	4.560	0.109	0.434	0.359
S12	0.044	0.652	0.615	4.705	0.182	0.425	0.035	4.097	2.159	0.147	0.248	0.344
S13	0.431	0.509	0.480	7.852	0.235	0.114	0.324	3.255	3.129	0.067	0.227	0.704
S14	0.202	0.585	0.426	9.472	0.055	0.044	0.177	7.043	1.955	0.105	0.207	0.806
S15	0.101	0.622	0.434	8.068	0.096	0.186	0.108	4.949	6.197	0.132	0.318	0.554
平均	0.350	0.464	0.424	6.174	0.056	0.091	0.129	5.427	3.605	0.116	0.188	0.502

基于HPLC指纹图谱和化学计量学的蒲公英质量评价研究

Study on Quality Evaluation of Dandelion Based on HPLC Fingerprint and Chemometrics

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