中草药虎杖离体快繁体系的优化

doi:10.19586/j.2095-2341.2023.0105

生物技术进展 ›› 2026, Vol. 16 ›› Issue (4): 934-940.DOI: 10.19586/j.2095-2341.2023.0105

中草药虎杖离体快繁体系的优化

刘铭¹^,²(), 张峻鑫¹^,², 贺志敏¹^,², 于丽平¹^,², 黄体冉¹^,²(), 马兰青¹^,²^,³(), 段留生⁴

^1.北京农学院生物与资源环境学院，北京 102206
^2.农业农村部华北都市农业重点实验室，北京 102206
^3.北京警察学院刑事科学技术系，北京 102202
^4.北京农学院植物科学技术学院，北京 102206

收稿日期:2023-09-11 接受日期:2023-11-08 出版日期:2026-07-25 发布日期:2026-09-11
通信作者: 黄体冉,马兰青
作者简介:刘铭E-mail: 1716097950@qq.com
基金资助:
北京市教育委员会科研计划一般项目(KM201910020016)

Optimization of Rapid Propagation System of Chinese Herbal Medicine Reynoutria japonica Houtt. in Vitro

Ming LIU¹^,²(), Junxin ZHANG¹^,², Zhimin HE¹^,², Liping YU¹^,², Tiran HUANG¹^,²(), Lanqing MA¹^,²^,³(), Liusheng DUAN⁴

^1.Bioscience and Resources Environment，Beijing University of Agriculture College，Beijing 102206，China
^2.Key Laboratory for Northern Urban Agriculture of Ministry of Agriculture and Rural Affairs，Beijing 102206，China
^3.Department of Criminal Science and Technology，Beijing Police College，Beijing 102202，China
^4.College of Plant Science and Technology，Beijing University of Agriculture，Beijing 102206，China

Received:2023-09-11 Accepted:2023-11-08 Online:2026-07-25 Published:2026-09-11
Contact: Tiran HUANG,Lanqing MA

摘要/Abstract

摘要：

为快速扩繁高质量虎杖种苗，优化虎杖离体组织快繁体系，以带腋芽虎杖茎段为外植体，探究了不同消毒时间和不同激素浓度对虎杖茎段增殖诱导和不定芽生根的影响。结果表明，虎杖带腋芽茎段的最佳灭菌方式为75%酒精15 s+(0.1%吐温-20+2%次氯酸钠)震荡清洗12 min，无菌外植体的获得率达到90.89%。诱导茎段腋芽出芽的最适培养基为MS+30 g·L^-1蔗糖+7 g·L^-1琼脂+1.5 mg·L^-1激动素+0.2 mg·L^-1萘乙酸，增殖率达到113.3%。不定芽生根培养的最适培养基为1/2 MS+30 g·L^-1蔗糖+5.5 g·L^-1琼脂+3 mg·L^-1绿色植物生长调节剂GGR6号生根率可达70.37%，且3 d左右分化出不定根，15 d左右获得完整植株；生根后组培苗的移栽成活率达到100%。研究结果优化了虎杖茎段组培快繁体系，无菌外植体获得率、增殖率和移栽成活率均超过90%，并且生根时间快，可显著缩短移栽时间，降低繁殖成本。

关键词: 虎杖, 离体快繁, 绿色植物生长调节剂

Abstract:

To rapid propagate high-quality Reynoutria japonica Houtt. seedlings and optimize a rapid propagation system of Reynoutria japonica Houtt. in vitro tissue， the effects of different disinfection times on the stem segments of Reynoutria japonica Houtt. and different hormone concentrations in the culture medium on the proliferation induction and adventitious bud rooting of the stem segments of Reynoutria japonica Houtt. were investigated. The results showed that the best sterilization method for the stem segment with axillary buds of Reynoutria japonica Houtt. was 75% alcohol 15 s+（0.1% Tween-20+2% sodium hypochlorite） for 12 min， and the acquisition rate of sterile explants reached 90.89%. The optimal medium for inducing axillary bud sprouting in stem segments was MS+30 g·L^-1 sucrose+7 g·L^-1 agar+1.5 mg·L^-1 kinetin+0.2 mg·L^-1 naphthalene acetic acid， with a proliferation rate of 113.3%. The optimal medium for rooting culture of adventitious buds was 1/2 MS+30 g·L^-1 sucrose+5.5 g·L^-1 agar+3 mg·L^-1 green plant growth regulator （GGR6）. The rooting rate could reach 70.37% and adventitious roots could be differentiated after about 3 days， and complete plants could be obtained after about 15 days. The survival rate of transplanted tissue cultured seedlings after rooting reached 100%. This study optimized a rapid culture system for stem segments of Reynoutria japonica Houtt.， with a sterile explant acquisition rate， proliferation rate， and transplant survival rate of over 90%. Moreover， the rooting time is fast， which can significantly shorten the transplant time and reduce reproduction costs.

Key words: Reynoutria japonica Houtt., rapid propagation in vitro, green plant growth regulator

中图分类号:

Q945.5

刘铭, 张峻鑫, 贺志敏, 于丽平, 黄体冉, 马兰青, 段留生. 中草药虎杖离体快繁体系的优化[J]. 生物技术进展, 2026, 16(4): 934-940.

Ming LIU, Junxin ZHANG, Zhimin HE, Liping YU, Tiran HUANG, Lanqing MA, Liusheng DUAN. Optimization of Rapid Propagation System of Chinese Herbal Medicine Reynoutria japonica Houtt. in Vitro[J]. Current Biotechnology, 2026, 16(4): 934-940.

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https://swjsjz.magtechjournal.com/CN/Y2026/V16/I4/934

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消毒时间/min	接种个数	培养时间/d	发芽率/%	污染率/%	死亡率/%	无菌外植体获得率/%
10	40	20	98.31±0.68a	23.73±9.63a	1.69±0.64b	74.58±3.93a
12	40	20	94.36±0.76a	3.47±0.71b	5.64±0.75a	90.89±6.73a
15	40	20	88.99±0.87a	3.13±0.89b	11.01±0.86a	86.90±7.30a
18	40	20	76.83±3.84a	7.68±1.07b	10.97±3.84a	84.68±8.51a

消毒时间/min	接种个数	培养时间/d	发芽率/%	污染率/%	死亡率/%	无菌外植体获得率/%
10	40	20	98.31±0.68a	23.73±9.63a	1.69±0.64b	74.58±3.93a
12	40	20	94.36±0.76a	3.47±0.71b	5.64±0.75a	90.89±6.73a
15	40	20	88.99±0.87a	3.13±0.89b	11.01±0.86a	86.90±7.30a
18	40	20	76.83±3.84a	7.68±1.07b	10.97±3.84a	84.68±8.51a

处理	KT/(mg·L^-1)	NAA/(mg·L^-1)	接种个数	培养时间/d	增殖率/%
1	1.0	0.2	40	30	87.5±9.01bc
2	1.5	0.4	40	30	99.2±7.64b
3	2.0	0.6	40	30	93.3±12.6b
4	1.0	0.4	40	30	80.0±9.01c
5	1.5	0.6	40	30	86.7±1.44bc
6	2.0	0.2	40	30	105.8±15.3a
7	1.0	0.6	40	30	58.3±21.8d
8	1.5	0.2	40	30	113.3±11.8a
9	2.0	0.4	40	30	91.7±6.3b

处理	KT/(mg·L^-1)	NAA/(mg·L^-1)	接种个数	培养时间/d	增殖率/%
1	1.0	0.2	40	30	87.5±9.01bc
2	1.5	0.4	40	30	99.2±7.64b
3	2.0	0.6	40	30	93.3±12.6b
4	1.0	0.4	40	30	80.0±9.01c
5	1.5	0.6	40	30	86.7±1.44bc
6	2.0	0.2	40	30	105.8±15.3a
7	1.0	0.6	40	30	58.3±21.8d
8	1.5	0.2	40	30	113.3±11.8a
9	2.0	0.4	40	30	91.7±6.3b

处理	AgNO₃/(mg·L^-1)	KT/(mg·L^-1)	NAA/(mg·L^-1)	接种个数	培养时间/d	增殖率/%
1	0	1.0	0.2	40	30	46.67±23.63b
2	0	1.5	0.4	40	30	47.50±10.00b
3	0	2.0	0.6	40	30	40.38±7.63bc
4	0.8	1.0	0.4	40	30	51.67±12.83ab
5	0.8	1.5	0.6	40	30	35.00±17.32bc
6	0.8	2.0	0.2	40	30	71.67±5.77a
7	1.6	1.0	0.6	40	30	30.83±5.20b
8	1.6	1.5	0.2	40	30	58.33±7.22a
9	1.6	2.0	0.4	40	30	38.33±8.78b

中草药虎杖离体快繁体系的优化

Optimization of Rapid Propagation System of Chinese Herbal Medicine Reynoutria japonica Houtt. in Vitro

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激素处理	接种个数	生根率/%	移栽成活率/%	观察现象
IBA	45	51.11	100	生根培养30 d，根长且粗壮，但出根慢，培养时间长
GGR6号	45	51.11	100	生根培养15 d，根长，多为3根左右，培养3 d可以观察到出根点

GGR6号/(mg·L^-1)	接种个数	培养时间/d	生根率/%	移栽成活率/%
3.0	20	15	70.37±16.97a	100±0.00
4.0	20	15	66.67±11.11ab	100±0.00
5.0	20	15	59.26±12.83ab	100±0.00
6.0	20	15	44.44±11.11b	100±0.00
7.0	20	15	59.26±6.42ab	100±0.00