Abstract: Bee pupa polypeptide has attracted wide attention because of its rich nutritional value and biological activities such as immune enhancement, anti-tumor and anti-oxidation. However, there is no report on the immunomodulatory activity of purified components of bee pupa polypeptide in vitro. In order to explore the effect of bee pupa polypeptide on the immune activity of macrophage RAW264.7, BPP-21, a purified component of bee pupa polypeptide, was taken as the research object, and the effects of BPP-21 at different concentrations (12.5, 25, 50, 100 and 200 μg·mL-1) on cell viability, phagocytosis, cytokine secretion, NO secretion and oxidative stress indexes of RAW264.7 were studied. The results showed that BPP-21 had no significant inhibitory activity on RAW264.7 cells within the concentrations ranged from 12.5 to 200 μg·mL-1, and significantly increased nitric oxide (NO) and interferon-γ (IFN-γ) secretion (P<0.05). In the range of 25~200 μg·mL-1, BPP-21 could significantly increase phagocytic activity, as well as interleukin-2 (IL-2) and tumor necrosis factor-α (TNF-α) secretion (P<005). In the range of 50~200 μg·mL-1, the activity of superoxide dismutase (SOD) in cells was significantly increased (P<005). The results showed that BPP-21, a purified component of bee pupa polypeptide, could enhance the immune activity of RAW264.7 macrophages, which provided a theoretical basis for the research and development of bee pupa polypeptide immunomodulator.

Key words: bee pupa, polypeptide, RAW264.7 cells, immune activity, oxidative stress

摘要： 蜂蛹多肽因具有丰富的营养价值，以及增强免疫、抗肿瘤及抗氧化等生物学活性，而受到了广泛关注，但目前关于蜂蛹多肽纯化组分的体外免疫调节活性的研究尚未见报道。为了探究蜂蛹多肽对巨噬细胞RAW264.7免疫活性的影响，以蜂蛹多肽纯化组分BPP-21为研究对象，研究其在不同浓度（12.5、25、50、100和200 μg·mL-1）下对RAW264.7巨噬细胞的细胞活力、吞噬能力、细胞因子分泌能力、NO分泌能力和氧化应激指标的影响。结果显示，在浓度12.5~200 μg·mL-1范围内，BPP-21对RAW264.7巨噬细胞无明显的细胞毒性作用，可显著提高干扰素-γ（interferon-gamma，IFN-γ）与NO的分泌水平（P<0.05）；在浓度25~200 μg·mL-1范围内，显著增加细胞吞噬能力以及白细胞介素-2（interleukin-2，IL-2）、肿瘤坏死因子α（tumor necrosis factor-alpha，TNF-α）的分泌量（P<0.05）；在浓度50~200 μg·mL-1范围内，显著提高细胞内超氧化物歧化酶（superoxide dismutase，SOD）活力（P<0.05）。研究表明，蜂蛹多肽纯化组分BPP-21可增强RAW264.7巨噬细胞的免疫活性，为蜂蛹多肽免疫调节剂的研究与开发提供了理论依据。

关键词: 蜂蛹, 多肽, RAW264.7细胞, 免疫活性, 氧化应激