Research Progress and Safety Evaluation of Transgenic Corn

doi:10.19586/j.2095-2341.2021.0196

Current Biotechnology ›› 2022, Vol. 12 ›› Issue (4): 523-531.DOI: 10.19586/j.2095-2341.2021.0196

• Reviews • Previous Articles Next Articles

Research Progress and Safety Evaluation of Transgenic Corn

Tingting LIU(), Tao TONG(), Kunlun HUANG()

Key Laboratory of Precision Nutrition and Food Quality，Key Laboratory of Functional Dairy，Ministry of Education，College of Food Science and Nutritional Engineering，China Agricultural University，Beijing 100083，China

Received:2021-12-17 Accepted:2022-01-19 Online:2022-07-25 Published:2022-08-10
Contact: Tao TONG,Kunlun HUANG

转基因玉米的研究进展和食用安全性评价

刘婷婷(), 仝涛(), 黄昆仑()

中国农业大学食品科学与营养工程学院，精准营养与食品质量重点实验室；教育部功能乳品重点实验室，北京 100083

通讯作者: 仝涛,黄昆仑
作者简介:刘婷婷 E-mail：SY20213061130@cau.edu.cn
基金资助:
北京市自然科学基金项目(7222249);山东省自然科学基金项目(ZR2021QC118);中国农业大学 2115 人才工程资助项目

Abstract

Abstract:

Corn is an important food crop and feed crop in China. At present， transgenic technology as a representative of biological breeding technology， has become the frontier and core technology in commercial breeding program. Transgenic corn with various excellent traits has been developed by transgenic technology in the world， which has created huge economic benefits. At the same time， unified and effective regulatory measures are important base for the genetic researches， development， extension and commercialization of genetically modified corn. Safety evaluation is the precondition for effective regulation. In this study， the commercialization progress of genetically modified corn and the research progress in transgene for important traits of corn were reviewed， and the food safety evaluation of genetically modified corn was summarized， which aimed to provide theoretical reference for research， management and promotion of genetically modified corn in China.

Key words: transgenic corn, biological breeding, regulatory measures, food safety

摘要：

玉米是我国重要的粮食作物和饲料作物。目前转基因技术作为生物育种技术的代表，已成为国际上育种的前沿和核心技术。世界各国利用转基因技术相继研发了具有多种优良性状的转基因玉米，创造了巨大的经济效益；同时统一、有效的监管措施是转基因玉米研发、推广和商业化的重要基础。食用安全性评价是有效监管的前提。针对转基因玉米的商业化和控制玉米重要性状基因的研究进展进行了综述，并对转基因玉米的食用安全性评价进行归纳分析，以期为我国转基因玉米的研发、管理和推广提供理论参考。

关键词: 转基因玉米, 生物育种, 监管措施, 食用安全性

CLC Number:

S513

Tingting LIU, Tao TONG, Kunlun HUANG. Research Progress and Safety Evaluation of Transgenic Corn[J]. Current Biotechnology, 2022, 12(4): 523-531.

刘婷婷, 仝涛, 黄昆仑. 转基因玉米的研究进展和食用安全性评价[J]. 生物技术进展, 2022, 12(4): 523-531.

References 65

1	路子显.近六十年我国玉米产业发展、贸易变化与未来展望[J].黑龙江粮食,2021(9):9-14.
2	梁晋刚,张旭冬,毕研哲,等.转基因抗虫玉米发展现状与展望[J].中国生物工程杂志,2021,41(6):98-104.
3	郭奥楠,杨学达,花薇.铁岭地区玉米主要病害发生危害调查分析[J].农业开发与装备,2021(10):165-166.
4	张正岩.中国转基因玉米技术商业化的风险预判研究[D].哈尔滨:东北农业大学,2018.
5	田文芳,赵国云,李丹.科学看待转基因[J].现代农村科技,2021(8):118.
6	International Service for the Acquisition of Agribiotech Applications (ISAAA). Global status of commercialized biotech/GM crops in 2018: biotech crops continue to help meet the challenges of increased population and climate change[R/OL].(2021-06-18)‍[2022-01-19]. .
7	焦悦,韩宇,杨桥,等.全球转基因玉米商业化发展态势概述及启示[J].生物技术通报,2021,37(4):164-76.
8	转基因权威关注.审批信息[EB/OL].(2017-08-02)[2020-02-14]. .
9	中华人民共和国农业农村部.2020年农业转基因生物安全证书批准清单[EB/OL].(2020-07-15) [2020-07-15]..
10	李梦杰, 贺晓云, 仝涛. 阿根廷转基因作物安全管理制度概况及进展 [J]. 生物技术进展, 2021, 11(6): 676-687.
11	中国农业信息网. 历年玉米进出口总值变动情况[DB/OL]. (2016-06-02)[2022-01-19]. .
12	丁云秀. 全球转基因玉米商业化应用现状及产品线研发进展 [J]. 农民致富之友,2016 (6): 99,25.
13	黎裕, 王天宇. 玉米转基因技术研发与应用现状及展望 [J]. 玉米科学, 2018, 26(2): 1-15, 22
14	DU D G C, ZHANG X. Transgenic maize lines expressing a cry1C* gene are resistant to insect pests [J]. Plant Mol. Biol. Rep., 2014, 32: 549-557.
15	QUAN R D, SHANG M, ZHANG H, et al.. Engineering of enhanced glycine betaine synthesis improves drought tolerance in maize [J]. Plant Biotechnol. J., 2004, 2(6): 477-486.
16	LI B, WEI A Y, SONG C X, et al.. Heterologous expression of the TsVP gene improves the drought resistance of maize [J]. Plant Biotechnol. J., 2008, 6(2): 146-159.
17	YANG L Y, LIU X L, GUO X Y, et al.. Comparison between transgenic maize with exotic betaine aldehyde dehydrogenase (BADH) gene and its untransformed counterpart [J]. Maydica, 2016, 61(2): 1-5.
18	刘成,杨炳鹏,孙宝成,等.转LOS5玉米的大田抗旱性鉴定[J].中国农业科学,2016,49(23):4469-4479.
19	刘岩,王国英,刘俊君,等.大肠杆菌gutD基因转入玉米及耐盐转基因植株的获得[J].中国科学C辑(生命科学),1998(6):542-547.
20	杨爱芳,张可炜,尹小燕,等.转基因耐盐玉米自交系的农艺性状及杂种优势表现的分析[J].中国农业科学,2007(12):2895-2902.
21	张举仁,李朝霞,解光宁.一种玉米mZmDEP基因和其表达抑制结构在玉米抗逆育种中的应用[P].中国:CN105349551B, 2019-07-02.
22	白云凤,赵晋锋,郑军,等.反义外壳蛋白基因介导的抗SCMV转基因玉米研究[J].作物学报,2006(5):661-665.
23	BAI Y, YANG H, QU L, et al.. Inverted-repeat transgenic maize plants resistant to sugarcane mosaic virus [J]. Acta Agron. Sin., 2007, 33: 973-978.
24	MIKEL M A, DARCY C J, RHODES A M, et al.. Inheritance of resistance to maize-dwarf mosaic-virus [J]. Phytopathology, 1983, 73(5): 792-795.
25	CAO X L, LU Y G, DI D P, et al.. Enhanced virus resistance in transgenic maize expressing a dsrna-specific endoribonuclease gene from E. coli [J/OL]. PLoS ONE, 2013, 8(4): e60829[2022-04-14]. .
26	SHEPHERD D N, MANGWENDE T, MARTIN D P, et al.. Maize streak virus-resistant transgenic maize: a first for Africa [J]. Plant Biotechnol. J., 2007, 5(6): 759-767.
27	ALLEN A, ISLAMOVIC E, KAUR J, et al.. Transgenic maize plants expressing the Totivirus antifungal protein, KP4, are highly resistant to corn smut [J]. Plant Biotechnol. J., 2011, 9(8): 857-864.
28	SUCHER J, BONI R, YANG P, et al.. The durable wheat disease resistance gene Lr34 confers common rust and northern corn leaf blight resistance in maize [J]. Plant Biotechnol. J., 2017, 15(4): 489-496.
29	THAKARE D, ZHANG J W, WING R A, et al.. Aflatoxin-free transgenic maize using host-induced gene silencing [J/OL]. Sci. Adv., 2017: 3(3): e1602382[2022-04-14]. .
30	LIU C, LI S X, YUE J, et al.. Microtubule-associated protein SBgLR facilitates storage protein deposition and its expression leads to lysine content increase in transgenic maize endosperm [J]. Int. J. Mol. Sci., 2015, 16(12): 29772-29786.
31	HUANG S, FRIZZI A, FLORIDA C A, et al.. High lysine and high tryptophan transgenic maize resulting from the reduction of both 19- and 22-kD alpha-zeins [J]. Plant Mol. Biol., 2006, 61(3): 525-535.
32	PLANTA J, XIANG X L, LEUSTEK T, et al.. Engineering sulfur storage in maize seed proteins without apparent yield loss [J]. Proc. Natl. Acad. Sci. USA, 2017, 114(43): 11386-11391.
33	RASCON-CRUZ Q, SINAGAWA-GARCIA S, OSUNA-CASTRO J A, et al.. Accumulation, assembly, and digestibility of amarantin expressed in transgenic tropical maize [J]. Theor. Appl. Genet., 2004, 108(2): 335-342.
34	OAKES J, BRACKENRIDGE D, COLLETTI R, et al.. Expression of fungal diacylglycerol acyltransferase2 genes to increase kernel oil in maize [J]. Plant Physiol., 2011, 155(3): 1146-1157.
35	SHEN B, ALLEN W B, ZHENG P Z, et al.. Expression of ZmLEC1 and ZmWRI1 increases seed oil production in maize [J]. Plant Physiol., 2010, 153(3): 980-987.
36	FARRE G, PEREZ-FONS L, DECOURCELLE M, et al.. Metabolic engineering of astaxanthin biosynthesis in maize endosperm and characterization of a prototype high oil hybrid [J]. Transgenic Res., 2016, 25(4): 477-489.
37	NAQVI S, ZHU C F, FARRE G, et al.. Transgenic multivitamin corn through biofortification of endosperm with three vitamins representing three distinct metabolic pathways [J]. Proc. Natl. Acad. Sci. USA, 2009, 106(19): 7762-7767.
38	ZHANG L, LUO Y Z, ZHU Y X, et al.. GmTMT2a from soybean elevates the alpha-tocopherol content in corn and Arabidopsis [J]. Transgenic. Res., 2013, 22(5): 1021-1028.
39	BI Y J, SUN Z C, ZHANG J, et al.. Manipulating the expression of a cell wall invertase gene increases grain yield in maize [J]. Plant Growth Regul., 2018, 84(1): 37-43.
40	WANG Z Y, CHEN X P, WANG J H, et al.. Increasing maize seed weight by enhancing the cytoplasmic ADP-glucose pyrophosphorylase activity in transgenic maize plants [J]. Plant Cell Tissue Organ. Cult., 2007, 88(1): 83-92.
41	NAHAMPUN H N, LEE C J, JANE J L, et al.. Ectopic expression of bacterial amylopullulanase enhances bioethanol production from maize grain [J]. Plant Cell Rep., 2013, 32(9): 1393-405.
42	孙越,刘秀霞,李丽莉,等.兼抗虫、除草剂、干旱转基因玉米的获得和鉴定[J].中国农业科学,2015,48(2):215-228.
43	余桂容,曹颖,杜文平,等.抗虫耐草甘膦双价基因表达载体的构建及玉米转化[J].西南农业学报,2015,28(2):475-479.
44	王国义,贺晓云,许文涛,等.转基因植物食用安全性评估与监管研究进展[J].食品科学,2019,40(11):343-350.
45	王瑶,卢佳希,胡贻椿,等.转Cry1Ab⁃ma基因玉米对大鼠的亚慢性毒性研究[J].中国食物与营养,2022,28(1):10-16.
46	ZHU Y, HE X, LUO Y, et al.. A 90-day feeding study of glyphosate-tolerant maize with the G2⁃aroA gene in Sprague-Dawley rats [J]. Food Chem. Toxicol., 2013: 51: 280-287.
47	张宇,令狐丽琴,胡贻椿,等.转AO基因高植酸酶玉米营养学评价分析[J].中国食物与营养,2017,23(5):50-54.
48	韩军花,杨月欣.转基因食品中的天然毒素与抗营养素[J].中国食品学报,2005(1):82-88.
49	蔡磊明.转基因抗虫玉米Bt-176食用安全性研究[J].现代农药,2003,2(1):27-30.
50	朱亚熙.转G2-aroA基因耐草甘膦玉米和非转基因玉米营养成分的比较分析[J].中国食物与营养,2012,18(9):65-69.
51	吴凯晋.转基因抗矮花叶玉米对大鼠部分亚慢性毒性指标的影响[J].安徽农业科学,2010,38(12):6224-6226.
52	金红,王永,程奕.深加工转基因产品检测技术的研究进展[J].天津农业科学,2003,9(4):32-35.
53	王卫国,过世东.GM饲料DNA在加工和畜禽消化道中的降解——转基因饲料安全性评价[J].粮食与饲料工业,2004(10):38-40.
54	MURRAY S R, BUTLER R C, TIMMERMAN-VAUGHAN G M. Quantitative real-time PCR assays to detect DNA degradation in soy-based food products [J]. J. Sci. Food Agric., 2009, 89(7): 1137-1144.
55	FERNANDES T J R, OLIVEIRA M B P P, MAFRA I. Tracing transgenic maize as affected by breadmaking process and raw material for the production of a traditional maize bread, broa [J]. Food Chem., 2013, 138(1): 687-692.
56	覃文,曹际娟,朱水芳.加工产品中转基因玉米Bt11成分实时荧光PCR定量(性)检测[J].生物技术通报,2003(6):46-50.
57	李欣竹.Cry1Ie蛋白的模拟胃肠液消化稳定性及热稳定性分析[J].生物技术通报,2015,31(11):214-221.
58	陈德龙,张莳眉,邹世颖,等.转基因耐草甘膦除草剂玉米CC-2喂养SD大鼠90天亚慢性毒性研究[J].农业生物技术学报,2013,21(12):1448-1457.
59	贺晓云.抗玉米根虫的转基因DAS-59122-7玉米和非转基因玉米SD大鼠90天喂养实验的结果比较[J].农业生物技术学报,2013,21(12):1533.
60	APPENZELLER L M, MALLEY L L, MACKENZIE S A, et al.. Subchronic feeding study with genetically modified stacked trait lepidopteran and coleopteran resistant (DAS-Ø15Ø7-1xDAS-59122-7) maize grain in Sprague-Dawley rats [J]. Food Chem. Toxicol., 2009, 47(7): 1512-1520.
61	郭梦凡.转Cry1Ab和epsps基因玉米的大鼠三代生殖毒性和子代神经发育毒性评价[D].北京:中国疾病预防控制中心,2018.
62	Food and Agriculture Organization of the United Nations/World Health Organization. Evaluation of allergenicity of genetically modiﬁed foods[R]. Report of a joint FAO/WHO expert consultation of allergenicity of foods derived from biotechnology. Italy: Food and Agriculture Organization of the United Nations, 2001.
63	卢云.转基因植物中潮霉素B磷酸转移酶的原核表达以及致敏性评估[J].农业生物技术学报,2013,21(12):1529.
64	赵浩含,王建华,刘允军.CP4-EPSPS蛋白的模拟胃肠液消化稳定性分析[J].生物技术,2017,27(2):180-185, 204.
65	王玉.AM79EPSPS蛋白的体外模拟胃肠液消化稳定性研究[J].生物技术通报,2017,33(6):176-181.

[1]	Zhenhua XU, Shiwei GAO, Dawei GAO, Yanming YU, Haiying LIU, Hongtao WU, Shuli ZHANG, Zhongyi SUN, Xin WANG, Ping YAN. Biological Breeding Science and Technology Innovation for Seed Industry Development and Prospects [J]. Current Biotechnology, 2025, 15(4): 557-564.
[2]	Ziya GAO, Bingxin HUANGFU, Jiake LI, Fangqian LI, Zhihao MA, Yang PANG, Jingang LIANG, Xiaoyun HE. Risk Identification and Suggestions for the Industrialization of Biological Breeding——based on Grower Field Research [J]. Current Biotechnology, 2025, 15(3): 456-465.
[3]	Jing WANG, Haitao GUAN, Xiaolei ZHANG, Baohuai WANG, Baohai LIU, Hongtao WEN. Detection Dynamic and Development Tendency of Agricultural Gene Editing Products [J]. Current Biotechnology, 2024, 14(5): 712-723.
[4]	Kehao CAO, Junli ZHU, Huashan HE, Weizhuo XU. Impact of the Fourth Modifications of Patent Laws on Biotechnology Patent Applications and Industry Development [J]. Current Biotechnology, 2023, 13(5): 663-670.
[5]	Enze CHENG-CHEN, Minghong JIA, Yueying LI. Research Progress of Nucleic Acid Reference Materials of Food-borne Pathogenic Bacteria [J]. Current Biotechnology, 2023, 13(2): 195-200.
[6]	Doudou LEI, Runran MA, Jiabo WANG, Weijun KONG. Research Progress on New Biological Detection Technology for Pesticide Residues in Foods [J]. Current Biotechnology, 2023, 13(1): 1-10.
[7]	Xing DANG, Binwei ZHI, Kehao CAO, Tingting LIU, Biao CHEN, Yuanjie DING. Patent Analysis on Genetically Modified Maize Biological Breeding Technology and Development Suggestions [J]. Current Biotechnology, 2022, 12(4): 614-622.
[8]	Min LIN. The Development Course and Industrialization Countermeasure of Agricultural Biological Breeding Technology [J]. Current Biotechnology, 2021, 11(4): 405-417.
[9]	Feng ZHANG, Xiaoyun HE, Kunlun HUANG, Qihuai WANG. Food Safety Evaluation of Transgenic Herbicide Tolerant Soybean [J]. Current Biotechnology, 2021, 11(4): 489-495.
[10]	YU Yan-qin, DU Jing-qi, ZHAO Jun, YANG Wei-min*. Physicochemical Properties of Jujube Fruit Peel Red Pigment and it's Application in Food Additives [J]. Curr. Biotech., 2014, 4(2): 129-134.
[11]	WANG Chun-ling, Bruce Hammond*. Food Safety of Bt Cry Proteins from Biotechnology-derived Crops [J]. journal1, 2011, 1(4): 235-241.

Research Progress and Safety Evaluation of Transgenic Corn

转基因玉米的研究进展和食用安全性评价

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

References 65

Related Articles 11

Recommended Articles

Metrics

Comments