Optimization of Enzymatic Extraction Process of Salidroside by Response Surface Analysis

doi:10.19586/j.2095-2341.2022.0177

Current Biotechnology ›› 2023, Vol. 13 ›› Issue (3): 441-448.DOI: 10.19586/j.2095-2341.2022.0177

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Optimization of Enzymatic Extraction Process of Salidroside by Response Surface Analysis

Lihua ZHANG(), Yifan WANG, Xuyang ZHANG, Qianying LIU, Siqi GAO, Jian JIAO()

Beijing City University，Beijing 100083，China

Received:2022-11-02 Accepted:2023-04-28 Online:2023-05-25 Published:2023-06-12
Contact: Jian JIAO

响应面分析法优化酶法提取红景天苷的工艺研究

张立华(), 王一樊, 张旭阳, 刘千盈, 高思琦, 焦健()

北京城市学院，北京 100083

通讯作者: 焦健
作者简介:张立华 E-mail: zlh20083395@163.com；
基金资助:
北京城市学院种子基金(KYZZ202005)

Abstract

Abstract:

The aim of this study was to research the optimum condition on extraction process of salidroside from Rhodiola crenulata with the enzymatic of cellulase method. The response surface methodology（RSM） was conducted based on single factor tests and orthogonal experiments. The effect of enzyme dosage， enzymolysis temperature and enzymolysis pH on extraction rate were tested. The optimal extraction condition were enzyme dosage 0.36%， extraction temperature 45 ℃， extraction pH 5.5， the extraction rate was 1.39%±0.31%. In summary， this energy-saving and environment-friendly extraction process could significantly reduce the amount of cellulase， and effectively improve the extraction yield of salidroside. Therefore， this process can be applied in the extraction of salidroside from Rhodiola crenulata. The results provided a scientific reference for developing and utilization of salidroside from Rhodiola crenulata.

Key words: Rhodiola crenulata, salidroside, response surface methodology, cellulase

摘要：

为研究纤维素酶酶解法提取大花红景天中红景天苷的最佳工艺，在单因素和正交实验数据的基础上，以红景天苷得率为响应值，进行响应面分析设计，考察酶用量、酶解温度、酶解pH对红景天苷得率的影响。结果显示，最佳提取工艺条件为：纤维素酶用量为药材重量的0.36%，酶解温度45 ℃、酶解pH 5.5，提取率为1.39%±0.31%。研究结果表明，该提取方法可减少酶用量，节能环保，有效提高红景天苷的提取率，可应用于大花红景天中红景天苷的提取。研究结果可为大花红景天中红景天苷产品的开发及应用提供科学参考。

关键词: 大花红景天, 红景天苷, 响应面分析法, 纤维素酶

CLC Number:

S567.239

Lihua ZHANG, Yifan WANG, Xuyang ZHANG, Qianying LIU, Siqi GAO, Jian JIAO. Optimization of Enzymatic Extraction Process of Salidroside by Response Surface Analysis[J]. Current Biotechnology, 2023, 13(3): 441-448.

张立华, 王一樊, 张旭阳, 刘千盈, 高思琦, 焦健. 响应面分析法优化酶法提取红景天苷的工艺研究[J]. 生物技术进展, 2023, 13(3): 441-448.

Figures/Tables 14

Table 1 Factors and levels of orthogonal experimental design

水平	因素
水平	酶用量/%	酶解pH	酶解时间/min	料液比	酶解温度/℃
1	0.25	4.5	90	1∶15	50
2	0.50	5.0	120	1∶20	60

Table 2 Factors and levels of RSM design

因素	水平
因素	-1	0	1
A：酶用量/%	0.05	0.25	0.45
B：酶解温度/℃	40	50	60
C：酶解pH	3	5	7

Fig. 1 Standard curve of salidroside

Fig. 2 Effects of mesh numbers of powder on yield of salidroside

Fig. 3 Effects of material liquid ratio on yield of salidroside

Fig. 4 Effects of the mount of enzyme on yield of salidroside

Fig. 5 Effects of enzymatic hydrolysis temperature on yield of salidroside

Fig. 6 Effects of enzymatic hydrolysis pH on yield ofsalidroside

Fig. 7 Effects of enzymatic hydrolysis time on yield ofsalidroside

Fig. 8 Effects of washing solution pH on yield of salidroside

Table 3 Orthogonal experimental design and results of analysis

序号	因素					红景天苷/%
序号	A：酶用量/%	B：酶解pH	C：酶解时间/min	D：料液比	E：酶解温度/℃	红景天苷/%
1	0.25	4.5	90	1∶15	50	0.84
2	0.25	4.5	120	1∶20	60	1.28
3	0.25	5.0	120	1∶15	60	1.21
4	0.25	5.0	90	1∶20	50	1.35
5	0.50	4.5	120	1∶15	50	0.66
6	0.50	4.5	90	1∶20	60	0.86
7	0.50	5.0	90	1∶15	60	1.08
8	0.50	5.0	120	1∶20	50	0.85
k1	1.17	0.91	1.032 5	0.947 5	0.925
k2	0.862 5	1.122 5	1	1.085	1.107 5
R	0.307 5	0.212 5	0.032 5	0.137 5	0.182 5

Table 4 Design and results of RSM

实验编号	A：酶用量/%	B：酶解温度/℃	C：酶解pH	红景天苷/%
1	0	0	0	1.38
2	0	0	0	1.38
3	-1	1	0	1.22
4	-1	0	-1	1.03
5	-1	0	1	1.01
6	0	1	1	0.97
7	1	-1	0	1.31
8	0	0	0	1.46
9	0	0	0	1.35
10	0	1	-1	0.98
11	1	0	-1	1.10
12	0	-1	1	1.20
13	0	-1	-1	1.11
14	1	0	1	1.31
15	1	1	0	1.02
16	0	0	0	1.47
17	-1	-1	0	1.01

Table 5 The analysis of variance on yield

来源	平方和	自由度	均方	F值	P值
模型	0.47	9	0.053	11.53	0.002 0
A：酶用量	0.028	1	0.028	6.06	0.043 4
B：酶解温度	0.024	1	0.024	5.31	0.054 6
C：酶解pH	9.113E-003	1	9.113E-003	2.00	0.200 3
AB	0.063	1	0.063	13.71	0.007 6
AC	0.013	1	0.013	2.90	0.132 3
BC	2.500E-003	1	2.500E-003	0.55	0.483 0
A²	0.051	1	0.051	11.23	0.012 2
B²	0.10	1	0.10	22.99	0.002 0
C²	0.14	1	0.14	31.70	0.000 8
残差	0.032	7	4.558E-003
失拟项	0.020	3	6.808E-003	2.37	0.211 3
绝对误差	0.011	4	2.870E-003
总误差	0.50	16
R²=0.936 8	$R A d j 2$ =0.855 5	C.V.%=5.65

Table 5 The analysis of variance on yield

来源	平方和	自由度	均方	F值	P值
模型	0.47	9	0.053	11.53	0.002 0
A：酶用量	0.028	1	0.028	6.06	0.043 4
B：酶解温度	0.024	1	0.024	5.31	0.054 6
C：酶解pH	9.113E-003	1	9.113E-003	2.00	0.200 3
AB	0.063	1	0.063	13.71	0.007 6
AC	0.013	1	0.013	2.90	0.132 3
BC	2.500E-003	1	2.500E-003	0.55	0.483 0
A²	0.051	1	0.051	11.23	0.012 2
B²	0.10	1	0.10	22.99	0.002 0
C²	0.14	1	0.14	31.70	0.000 8
残差	0.032	7	4.558E-003
失拟项	0.020	3	6.808E-003	2.37	0.211 3
绝对误差	0.011	4	2.870E-003
总误差	0.50	16
R²=0.936 8	$R A d j 2$ =0.855 5	C.V.%=5.65

Fig. 9 Response surface map and contour map of interaction effect of enzyme quantity, enzymolysis temperature and enzymolysis time on the yield

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Optimization of Enzymatic Extraction Process of Salidroside by Response Surface Analysis

响应面分析法优化酶法提取红景天苷的工艺研究

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