Current Biotechnology ›› 2012, Vol. 2 ›› Issue (4): 270-275.DOI: 10.3969/j.issn.2095-2341.2012.04.06

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Study on Genetic Divergence of Hainan Common Wild Rice (Oraza rufipogon) Based on ITS Analysis

YIN Zhao-kun, LIAO Cheng-hong, XU Li-xin, PEI Xin-wu, YUAN Qian-hua   

  1. 1.College of Agriculture, Hainan University, Haikou 570228, China;
    2.Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2012-05-08 Online:2012-07-25 Published:2012-06-05

基于ITS序列分析对海南普通野生稻籼粳分化的研究

尹昭坤1,廖承红1,徐立新1,裴新梧2,袁潜华1*   

  1. 1.海南大学农学院, 海口 570228;
    2.中国农业科学院生物技术研究所, 北京 100081
  • 通讯作者: 袁潜华,研究员,主要从事转基因生物安全及作物遗传育种研究。E-mail:qhyuan@163.com
  • 作者简介:尹昭坤,硕士研究生,研究方向为植物生物技术与种质创新。E-mail:15248920982@163.com。
  • 基金资助:

    国家转基因生物新品种培育重大专项(2009ZX08011-010B)资助。

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Abstract: Eighteen individual samples of common wild rice (Oryza rufipogon Griff.) from Hainan were used for cloning and sequencing. Sequence analysis showed significant differences in ITS sequence among these populations. The results indicated that these populations had a genetic relationship, a total of 108 polymorphic fragments were detected in ITS1, and 145 polymorphic fragments in ITS2. Besides, there were 133 InDels in ITS1, 145 InDels in ITS2. It indicated that the common wild rice from Hainan performed some Indica-Japonica differentiations, more Indica than Japonica types, and 9 ITS Indica-Japonica differentiations sites were found. This work will help to understand the status of Oraza ruffipogon from Hainan in the origin and evolution of the cultivated rice in China, and also offer reference for potential use of the wild rice from Hainan.

Key words: Oraza rufipogon, ITS sequence, Indica-Japonica differentiation, Hainan

摘要： 对海南普通野生稻的18个居群分别进行ITS序列克隆和测序,研究海南普通野生稻居群的籼粳分化。结果表明,海南普通野生稻的ITS序列居群间差异性显著,ITS1长度为163~239 bp,G+C含量变化范围为53.5%~77-2%。ITS2长度为165~243 bp,G+C含量变化范围为54.5%~74.2%。ITS1和ITS2区简约信息位点分别为108个和145个,单一信息位点分别为7个和2个。InDel(插入/缺失)位点分别为133个和145个,发现了9个ITS籼粳特异性位点。ClustalX软件排序及Mega构树结果表明,海南普通野生稻存在籼粳分化,偏籼型有10个居群,偏粳型有8个居群。本研究有助于揭示海南普通野生稻在起源演化上的地位,并为有效利用海南优良野生稻种质资源提供理论依据。

关键词: 普通野生稻, ITS序列, 籼粳分化, 海南

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