Curr. Biotech. ›› 2016, Vol. 6 ›› Issue (5): 328-335.DOI: 10.3969/j.issn.2095-2341.2016.05.04

Previous Articles     Next Articles

Cloning and Expression Analysis of Maize Mediator Subunit Gene ZmMED7#br#

WANG Jing, ZHAO Jun, ZONG Na,   

  1. 1.Faculty of Maize Functional Genomics, Department of Crop Genome Research and Genetic Improvement, Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
    2.National Key Facility for Crop Gene Resources and Genetic Improvement, CAAS, Beijing 100081, China
  • Received:2016-04-25 Online:2016-09-25 Published:2016-06-03

玉米中介体亚基ZmMED7基因的克隆及表达分析

王晶1,2,赵军1,2,宗娜1,2*   

  1. 1.中国农业科学院生物技术研究所, 作物基因组与遗传改良研究室玉米功能基因组创新团队, 北京 100081;
    2.中国农业科学院, 农作物基因资源与基因改良国家重大科学工程, 北京 100081
  • 通讯作者: 宗娜，副研究员，博士，研究方向为植物抗逆性和籽粒发育分子生物学和分子育种研究。E-mail:zongna@caas.cn
  • 作者简介:王晶，硕士研究生，研究方向为植物抗逆分子生物学研究。E-mail:13051524392@163.com。
  • 基金资助:
    国家自然科学基金项目(31170235) 资助。

PDF

Abstract: To study the function of maize mediator subunit ZmMED7 gene. We used Real-time Quantitative PCR analysis to study the gene expression levels of ZmMED7-1 and ZmMED7-2 in different tissues and under the conditions of abiotic stress, such as ABA、NaCl and PEG treatment. Results showed that: The expressions of ZmMED7-1 and ZmMED7-2 could be detected in all the tissues sampled at different  development stages, but expression of ZmMED7-1 and ZmMED7-2 were highest at the leaves of V1 and V5 stage. Treated by ABA、NaCl and PEG all lead to decreased expression of ZmMED7-1 and ZmMED7-2. The germination of ZmMED7-1 over-expression transgenic Arabidopsis seeds showed more sensitive to salt stress treatment. ZmMED7 play negative regulator roles in the process of plants responding to salt stress treatment.

Key words: mediator complex, expression analysis, abiotic stress

摘要： 为了研究玉米中介体亚基ZmMED7基因的功能，通过荧光定量PCR技术,研究ZmMED7-1和ZmMED7-2在玉米不同组织中的表达及在ABA处理、盐害和渗透胁迫等逆境条件下的应答反应，并将ZmMED7-1和ZmMED7-2转入拟南芥中研究其基因功能。结果显示，ZmMED7在玉米不同发育时期的组织器官中均有表达，ZmMED7-1和ZmMED7-2分别在V1和V5期的叶片中表达量最高。ABA、盐害和渗透胁迫等处理都能够抑制ZmMED7-1和ZmMED7-2 基因的表达。过表达ZmMED7-1导致拟南芥在种子萌发时期对盐胁迫的耐受能力减弱。研究结果说明：ZmMED7基因作为负调控因子参与了植物逆境胁迫应答反应。

关键词: 转录中介体, 表达分析, 逆境胁迫

[an error occurred while processing this directive]