Abstract: To observe the influence of PAP on the IFN-γ and soluble LAG-3 molecule level of spleen lymphocyte supernatant  in mice which acute exposured to low doses of cadmium. The solution of cadmium chloride were injected into the intraperitoneal of the test mice with 1 mg/kg. After 24 h, the model had been successfully made, PAP of low, medium and high doses were injected into the intraperitoneal for a week, spleen index and thymus index were measured at the end of the experiment, the content of IFN-γ and sLAG-3 were measured by ELISA. The cells of CD3+,CD4+,CD8+ in spleen lymphocytes was observed by immunofluorescence. The results showed that, compared with the blank control and the cadmium stained positive control, the content of IFN-γ and sLAG-3 were increased among the different doses of PAP groups. The high concentration group increased the most obvious（P<0.05）, the amount of CD3+, CD4+ cells were also correspond to the number of rising but CD8+cells were not increased significantly. It was still the most significant increase in the high dose group. PAP could increase the content of IFN-γ and soluble LAG-3 of the mice acute exposuring to low doses of cadmium, and CD3+,CD4+ lymphocytes were in a corresponding increase.

Key words: PAP, IFN-γ, sLAG-3

摘要： 为了观察蕨麻多糖对急性低剂量镉染毒小鼠脾淋巴细胞上清液中IFN-γ和可溶性LAG-3（soluble LAG-3，sLAG-3）分子水平的影响。将试验小鼠一次性腹腔注射氯化镉溶液(1 mg/kg)染毒，观察24h即造模成功后，腹腔注射低、中、高剂量蕨麻多糖治疗一周，于实验终末测各组小鼠脾脏、胸腺指数，ELISA法测IFN-γ、sLAG-3含量；免疫荧光法观察CD3+、CD4+、CD8+T淋巴细胞。结果表明蕨麻多糖低、中、高剂量组中，IFN-γ、sLAG-3的含量较空白对照组和单纯镉染阳性对照组均增高，以高浓度组增高明显（P<0.05）；CD3+、CD4+T淋巴细胞较空白对照组和单纯镉染阳性对照组均增多，仍以高浓度组增多明显,CD8+T变化不显著。蕨麻多糖能促进急性低剂量镉染毒小鼠脾淋巴细胞分泌IFN-γ和sLAG-3，CD3+、CD4+T淋巴细胞亦相应增加。

关键词: 蕨麻多糖, IFN-&gamma, sLAG-3