Abstract: Under the tendency rapid development of geneticallly modified orgnism(GMO), it is necessary to obtain the marker-free transgenic plants. At present,the marker-free transgenic technologies include co-transformation,site-specific recombination, transposons, homologous recombination and so on. This paper summarizes the character, the theories and research progress of marker-free and redundant sequence-free transgenic technologies, which are extensively used in recent research. Then, it also prospects the application potential of the safe transgenic technologies for the rapid commercialization of GMOs.

Key words: selectable marker gene, co-transformation, site-specific recombination; , transposons, homologous recombination

摘要： 获得无选择标记基因的转基因作物是转基因作物发展的必然趋势。目前剔除选择标记基因的常用方法包括共转化、位点特异性重组、转座子、同源重组等。本文综述了这些常用技术的原理和目前的发展现状，同时对这些技术的特点进行描述，并对植物安全转基因技术发展进行展望，以期推动转基因作物的快速产业化。

关键词: 选择标记基因, 共转化, 位点特异性重组, 转座子, 同源重组