Abstract:

Pseudomonas aeruginosa is a conditionally pathogenic bacterium with greater infection risk and harm to patients. Establishing a detection method for P. aeruginosa in cold chain foods and completing the construction of a traceable molecular evolutionary tree is crucial for food safety. Real-time fluorescence PCR technology was used to establish a rapid detection method for P. aeruginosa in cold chain food. After the detection of P. aeruginosa through cold chain food sampling investigation， the strains detected in the samples were sequenced and analyzed， and a biological evolutionary tree of P. aeruginosa was established to complete the traceability analysis. As a result， a total of 10 pathogenic bacteria were successfully detected from 271 randomly collected cold chain foods using this method， and the overall detection rate of pathogenic bacteria was 3.69% （10/271）. The source of contamination of P. aeruginosa was successfully traced and the localization of various genera was completed. The research results could provide ideas and methods for the detection and traceability analysis of P. aeruginosa and other related pathogens in cold chain food.

Key words: cold chain food, Pseudomonas aeruginosa, biological evolutionary tree, real-time fluorescence PCR

摘要：

铜绿假单胞菌是条件致病菌，对于患者具有较大的感染风险与危害，因此建立冷链食品中铜绿假单胞菌的检测方法并完成其溯源分子进化树构建工作，对于保障食品安全至关重要。以冷链食品中的铜绿假单胞菌为研究对象，应用实时荧光PCR检测技术建立快速检测方法。通过冷链食品抽样调查检测出铜绿假单胞菌后，将样品中检测出的铜绿假单胞菌菌株进行测序分析并建立铜绿假单胞菌生物进化树完成溯源分析。结果利用该方法成功从随机采集的271份冷链食品中检出10株病原菌，病原菌总体检出率为3.69%(10/271)，并通过分子进化树的构建成功溯源铜绿假单胞菌的污染来源并完成菌种种属定位。研究成果可以为冷链食品中铜绿假单胞菌等相关病原菌的检测溯源分析提供思路与方法。

关键词: 冷链食品, 铜绿假单胞菌, 生物进化树, 实时荧光PCR检测