Application of Caenorhabditis elegans Model in the Study of Memory and Forgetting Behavior

doi:10.19586/j.2095-2341.2023.0077

Current Biotechnology ›› 2023, Vol. 13 ›› Issue (6): 837-843.DOI: 10.19586/j.2095-2341.2023.0077

• Reviews • Previous Articles Next Articles

Application of Caenorhabditis elegans Model in the Study of Memory and Forgetting Behavior

Xin ZHAO¹^,²(), Xinyu LI¹(), Minghao LI¹, Shiyi ZHOU¹, Yaqi DENG¹, Zhiyuan ZHENG¹, Wei ZOU¹()

^1.School of Public Health，Kunming Medical University，Kunming 650500，China
^2.Xi'an Public Health Center，Xi'an 710299，China

Received:2023-06-06 Accepted:2023-08-28 Online:2023-11-25 Published:2023-12-12
Contact: Wei ZOU

秀丽隐杆线虫模型在记忆和遗忘行为研究中的运用

赵歆¹^,²(), 李鑫玉¹(), 李明浩¹, 周诗艺¹, 邓雅琪¹, 郑至远¹, 邹伟¹()

^1.昆明医科大学公共卫生学院，昆明 650500
^2.西安市公共卫生中心，西安 710299

通讯作者: 邹伟
作者简介:赵歆E-mail: 798766529@qq.com
李鑫玉E-mail: xinyuliyx@163.com。第一联系人：（李鑫玉、赵歆并列第一作者）
基金资助:
云南省基础研究计划(昆医联合专项)(202301AY070001-194)

Abstract

Abstract:

Memory is the basis of learning and mastering new knowledge， and forgetting helps to maintain the efficiency of the brain memory system， so memory and forgetting are important components of the normal operation of the brain neural network. With its small size， short life cycle， and easy recognition of single neurons， Caenorhabditis elegans has become one of the ideal models for neuroscience and behavioral research. Studies based on C. elegans model combined with higher model organisms to explore the mechanisms of memory and forgetting will help reveal the occurrence of diseases related to abnormal memory and forgetting. In this paper， we reviewed the molecular mechanism of memory and forgetting behavior of C. elegans widely used in volatile substances and pathogenic bacteria， and the application of transgenic C. elegans in memory and forgetting related diseases， so as to provide theoretical reference for subsequent research on memory and forgetting.

Key words: Caenorhabditis elegans, memory, forgetting behavior, molecular mechanism

摘要：

记忆是学习和掌握新知识的基础，遗忘则有助于保持大脑记忆系统的高效性，因此记忆与遗忘是大脑神经网络正常运作的重要组成部分。秀丽隐杆线虫生物体积小、生命周期短、易于识别单个神经元，已成为神经科学和行为学领域研究的理想模型之一，基于秀丽隐杆线虫模型的研究结合高等模式生物探索记忆和遗忘的机制将有助于揭示记忆和遗忘异常相关疾病的发生。综述了秀丽隐杆线虫广泛用于挥发性物质与病原菌的记忆与遗忘行为的分子机制研究，以及转基因线虫在记忆与遗忘相关疾病中的应用，旨在为后续记忆和遗忘的研究提供理论参考。

关键词: 秀丽隐杆线虫, 记忆, 遗忘行为, 分子机制

CLC Number:

Xin ZHAO, Xinyu LI, Minghao LI, Shiyi ZHOU, Yaqi DENG, Zhiyuan ZHENG, Wei ZOU. Application of Caenorhabditis elegans Model in the Study of Memory and Forgetting Behavior[J]. Current Biotechnology, 2023, 13(6): 837-843.

赵歆, 李鑫玉, 李明浩, 周诗艺, 邓雅琪, 郑至远, 邹伟. 秀丽隐杆线虫模型在记忆和遗忘行为研究中的运用[J]. 生物技术进展, 2023, 13(6): 837-843.

References 53

1	ATKINSON R C, SHIFFRIN R M. Human memory: a proposed system and its control processes[M]// Psychology of Learning and Motivation. Amsterdam: Elsevier, 1968: 89-195.
2	ANDERMANE N, JOENSEN B H, HORNER A J. Forgetting across a hierarchy of episodic representations[J]. Curr. Opin. Neurobiol., 2021, 67: 50-57.
3	周启心,毛榕榕,徐林.令人烦恼的记忆[J].生命科学,2014,26(6):610-619.
4	FRANKLAND P W, KÖHLER S, JOSSELYN S A. Hippocampal neurogenesis and forgetting[J]. Trends Neurosci., 2013, 36(9): 497-503.
5	DAVIS R L, ZHONG Y. The biology of forgetting-a perspective[J]. Neuron, 2017, 95(3): 490-503.
6	张亚,田绍文.遗忘的神经机制及其与神经系统疾病的相关性[J].中国生物化学与分子生物学报,2021,37(2):169-175.
7	GRIFFIN E F, SCOPEL S E, STEPHEN C A, et al.. ApoE-associated modulation of neuroprotection from Aβ-mediated neurodegeneration in transgenic Caenorhabditis elegans [J/OL]. Dis. Model. Mech., 2019, 12(2): dmm037218[2019-02-15]. .
8	PRETSCH D, ROLLINGER J M, SCHMID A, et al.. Prolongation of metallothionein induction combats Aβ and α-synuclein toxicity in aged transgenic Caenorhabditis elegans [J/OL]. Sci. Rep., 2020, 10(1): 11707[2020-7-10]. .
9	罗盛,陈姚静,张占军.老年人记忆衰退的神经生物学基础研究进展[J].中华老年多器官疾病杂志,2022,21(12): 910-913.
10	王庆磊,陈艳梅,张继川.主动遗忘的细胞分子机制研究进展[J].基因组学与应用生物学,2021,40(S4):3863-3869.
11	张亚,田绍文.遗忘的神经机制及其与神经系统疾病的相关性[J].中国生物化学与分子生物学报,2021,37(2):169-175.
12	VAN DER KOLK B A, FISLER R. Dissociation and the fragmentary nature of traumatic memories: overview and exploratory study[J]. J. Trauma. Stress, 1995, 8(4): 505-525.
13	彭江,刘强.老年遗忘型轻度认知功能障碍对情节记忆影响[J].世界最新医学信息文摘,2018,18(67):43.
14	张莹,田升军,李志强,等.阿尔茨海默病与遗忘型轻度认知功能障碍患者的记忆损害[J].中国老年学杂志,2016,36(8): 1869-1870.
15	SCHAFER W R. Neurophysiological methods in C. elegans: an introduction[J]. WormBook, 2006: 1-4.
16	LEUNG M C, WILLIAMS P L, BENEDETTO A, et al.. Caenorhabditis elegans: an emerging model in biomedical and environmental toxicology[J]. Toxicol. Sci. Off. J. Soc. Toxicol., 2008, 106(1): 5-28.
17	GRANATO E T, ZIEGENHAIN C, MARVIG R L, et al.. Low spatial structure and selection against secreted virulence factors attenuates pathogenicity in Pseudomonas aeruginosa [J]. ISME J., 2018, 12(12): 2907-2918.
18	GRAY J M, HILL J J, BARGMANN C I. A circuit for navigation in Caenorhabditis elegans [J]. Proc. Natl. Acad. Sci. USA, 2005, 102(9): 3184-3191.
19	WIXTED J T. The psychology and neuroscience of forgetting[J]. Annu. Rev. Psychol., 2004, 55: 235-269.
20	CASTILLO DÍAZ F, CAFFINO L, FUMAGALLI F. Bidirectional role of dopamine in learning and memory-active forgetting[J]. Neurosci. Biobehav. Rev., 2021, 131: 953-963.
21	BAI H, HUANG H, ZHAO N, et al.. Small G protein RAC-2 regulates forgetting via the JNK-1 signalling pathway in Caenorhabditis elegans [J]. Eur. J. Neurosci., 2022, 56(12): 6162-6173.
22	赵娜,任长虹,刘虎岐,等.秀丽隐杆线虫学习行为研究方法[J].西北农林科技大学学报(自然科学版),2009,37(11):55-61.
23	YE H Y, YE B P, WANG D Y. Learning and learning choice in the nematode Caenorhabditis elegans [J]. Neurosci. Bull., 2006, 22(6): 355-360.
24	HAR A C, CHAO M Y. The neurobiology of olfaction[M]. Boca Raton: CRC Press/Taylor Francis Group, 2010.
25	BARGMANN C I, HARTWIEG E, HORVITZ H R. Odorant-selective genes and neurons mediate olfaction in C. elegans [J]. Cell, 1993, 74(3): 515-527.
26	INOUE A, SAWATARI E, HISAMOTO N, et al.. Forgetting in C. elegans is accelerated by neuronal communication via the TIR-1/JNK-1 pathway[J]. Cell Rep., 2013, 3(3): 808-819.
27	KAUFFMAN A L, ASHRAF J M, CORCES-ZIMMEMAN M R, et al.. Insulin signaling and dietary restriction differentially influence the decline of learning and memory with age[J/OL]. PLoS Biol., 2010, 8(5): e1000372[2010-05-18]. .
28	RAHMANI A, CHEW Y L. Investigating the molecular mechanisms of learning and memory using Caenorhabditis elegans [J]. J. Neurochem., 2021, 159(3): 417-451.
29	GOURGOU E, ADIGA K, GOETTEMOELLER A, et al.. Caenorhabditis elegans learning in a structured maze is a multisensory behavior[J/OL]. Iscience., 2021, 24(4): 102284[2021-04-23]. .
30	PEREIRA A G, GRACIDA X, KAGIAS K, et al.. C. elegans aversive olfactory learning generates diverse intergenerational effects[J]. J. Neurogenet., 2020, 34(3-4): 378-388.
31	MOORE R S, KALETSKY R, MURPHY C T. Protocol for transgenerational learned pathogen avoidance behavior assays in Caenorhabditis elegans [J/OL]. STAR Protoc., 2021, 2(1): 100384[2021-03-10]. .
32	HARRIS G, SHEN Y, HA H, et al.. Dissecting the signaling mechanisms underlying recognition and preference of food odors[J]. J. Neurosci. Off. J. Soc. Neurosci., 2014, 34(28): 9389-9403.
33	ZHANG Y, LU H, BARGMANN C I. Pathogenic bacteria induce aversive olfactory learning in Caenorhabditis elegans [J]. Nature, 2005, 438(7065): 179-184.
34	SHIVERS R P, KOOISTRA T, CHU S W, et al.. Tissue-specific activities of an immune signaling module regulate physiological responses to pathogenic and nutritional bacteria in C. elegans [J]. Cell Host Microbe, 2009, 6(4): 321-330.
35	LEE K, MYLONAKIS E. An intestine-derived neuropeptide controls avoidance behavior in Caenorhabditis elegans [J]. Cell Rep., 2017, 20(10): 2501-2512.
36	BAI H, ZOU W, ZHOU W, et al.. Deficiency of innate immunity against Pseudomonas aeruginosa enhances behavioral avoidance via the HECW-1/NPR-1 module in Caenorhabditis elegans [J/OL]. Infect. Immun., 2021, 89(10): e0006721[2021-09-16]. .
37	MELO J A, RUVKUN G. Inactivation of conserved C. elegans genes engages pathogen- and xenobiotic-associated defenses[J]. Cell, 2012, 149(2): 452-466.
38	KRAEMER P J, GOLDING J M. Adaptive forgetting in animals[J]. Psychon. Bull. Rev., 1997, 4(4): 480-491.
39	KANDEL E R, DUDAI Y, MAYFORD M R. The molecular and systems biology of memory[J]. Cell, 2014, 157(1): 163-186.
40	KITAZONO T, HARA-KUGE S, MATSUDA O, et al.. Multiple signaling pathways coordinately regulate forgetting of olfactory adaptation through control of sensory responses in Caenorhabditis elegans [J]. J. Neurosci. Off. J. Soc. Neurosci., 2017, 37(42): 10240-10251.
41	HADZISELIMOVIC N, VUKOJEVIC V, PETER F, et al.. Forgetting is regulated via Musashi-mediated translational control of the Arp2/3 complex[J]. Cell, 2014, 156(6): 1153-1166.
42	LIM J P, FEHLAUER H, DAS A, et al.. Loss of CaMKI function disrupts salt aversive learning in C. elegans [J]. J. Neurosci. Off. J. Soc. Neurosci., 2018, 38(27): 6114-6129.
43	CHEUNG B H, COHEN M, ROGERS C, et al.. Experience-dependent modulation of C. elegans behavior by ambient oxygen[J]. Curr. Biol., 2005, 15(10): 905-917.
44	王凤娟,方亚影,吴宿慧,等.东莨菪碱诱导秀丽线虫痴呆模型的特点探析[J].中药药理与临床,2021,37(1):207-211.
45	杨洋,王凤林,刘德,等.CRISPR-Cas9技术在植物次生代谢物生产中的研究进展[J].生物技术进展,2022,12(6):806-816.
46	LIANG P, XU Y, ZHANG X, et al.. CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes[J]. Protein Cell, 2015, 6(5): 363-372.
47	黄春蒙,朱鹏宇,王智,等.基于RNAi技术的转基因植物研究进展[J].生物技术进展,2020,10(1):1-9.
48	解啸,成飞雪,程菊娥,等.RNAi在植物寄生线虫中的研究及应用[J].基因组学与应用生物学,2015,34(5):1087-1091.
49	MUIRHEAD C S, SRINIVASAN J. Small molecule signals mediate social behaviors in C. elegans [J]. J. Neurogenet., 2020, 34(3-4): 395-403.
50	GRIFFIN E F, CALDWELL K A, CALDWELL G A. Genetic and pharmacological discovery for Alzheimer's disease using Caenorhabditis elegans [J]. ACS Chem. Neurosci., 2017, 8(12): 2596-2606.
51	薛晓利,张建琴,宋少娟,等.秀丽隐杆线虫衰老与衰老相关神经退行性疾病模型及药物筛选研究进展[J].中国药理学与毒理学杂志,2017,31(5):429-438.
52	曹绪文,张留所.CRISPR/Cas9基因编辑技术在线虫中的应用进展和展望[J].中国科学:生命科学,2018,48(5):513-520.
53	YEMINI E, LIN A, NEJATBAKHSH A, et al.. NeuroPAL: a multicolor atlas for whole-brain neuronal identification in C. elegans [J]. Cell, 2021, 184(1): 272-288.

[1]	Yunshuo CHENG, Zixu LI, Guanghua MAO, Xiangyang WU. Studies on the Immunological Effects of Dinotefuran Exposure on Zebrafish Juveniles [J]. Current Biotechnology, 2025, 15(3): 446-455.
[2]	Fan LIU, Chen CHEN, Yuanzhi ZHU, Yanjun FU, Lin WANG, Ting ZHANG, Shan WANG. Obstructive Sleep Apnea and Cognitive Impairment: A Review of Epigenetic Advances [J]. Current Biotechnology, 2024, 14(5): 805-812.
[3]	Wei ZHANG, Hongfang WANG, Baohua XU. Overview of the Main Molecular Mechanisms of Biological Aging [J]. Current Biotechnology, 2023, 13(2): 228-233.
[4]	Guizhen GAO, Yungu ZHAI, Lubin ZHANG, Jinmei CHANG, Haihua LUO, Xiaoming WU. Advances on Germplasm Identification and Variety Breeding to Saline-alkali Stress of Rapeseed [J]. Current Biotechnology, 2022, 12(5): 647-654.
[5]	Geru TAO, Shucun QIN. Molecular Mechanism of Hydrogen Biomedicine in Relieving Free Radical Oxidative Stress [J]. Current Biotechnology, 2022, 12(4): 490-496.
[6]	Xiangyang WANG, Anjun LIU, Jing ZHAO. Effects of Dexmedetomidine on Offspring Development, Spatial Learning and Memory Ability of Pregnancy Rats with Chronic Stress Depression [J]. Current Biotechnology, 2022, 12(1): 135-141.
[7]	YAO Mawulikplimi Adzavon, ZHAO Pengxiang*, ZHANG Xujuan, WANG Limin, MA Xuemei. Research Progress on Molecular Mechanism of Microphage Migration Inhibotry Factor [J]. Curr. Biotech., 2018, 8(5): 389-396.

Application of Caenorhabditis elegans Model in the Study of Memory and Forgetting Behavior

秀丽隐杆线虫模型在记忆和遗忘行为研究中的运用

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

References 53

Related Articles 7

Recommended Articles

Metrics

Comments