关键词: N-糖基化, N-糖链, N-糖组, 质谱, 植物糖生物学

Abstract: N-glycosylation is the most prevalent post-translational modification of proteins, and plays an important role in multiple biological processes, such as embryonic development, cancer development and immune defense. In recent years, the strategies for mass spectrometry-based identification and quantification of N-glycans have made significant progresses in animals. In contrast, the studies on detection of N-glycosylation and N-glycans in plants have lagged far behind, and this is also one of the key bottlenecks in the study of plant glycobiology. The release, quantitative strategy, visual detection of N-glycans and their application progresses in plants were reviewed in order to provide references for guiding the qualitative and quantitative detection of N-glycan and N-glycome in plants.

Key words: N-glycosylation, N-glycan, N-glycome, mass spectrometry, plant glycobiology