关键词: 莲雾, 气相色谱-质谱联用, 代谢产物, 代谢组学

Abstract: In order to estimate the effects of two extraction solvents on the metabolomics analysis of wax apple, Taiwan ‘Black Pearl’ wax apple fruits (Syzygium samarangense Merr. et Perry.) were used as materials, and two kinds of extraction solvents were selected to analyze the non-target metabolic profile of the fruit samples. Isolating and identifying the wax apple metabolites by Gas Chromatography-Mass Spectrometer(GC-MS), a total of 129 metabolites were identified through methanol-water method (A) and methanol-chloroform-water method(B) respectively. Principal component analysis (PCA) was used to compare the two extraction methods, which showed that there were significant differences between the two methods. From the total number of chromatographic peaks, 323 chromatographic peaks can be detected by method A while only 251 by method B. Statistical analysis showed that 124 metabolites with significant differences, of which the relative contents of 90 metabolites extracted by method A was significantly higher than those by method B. The results showed that, the methanol-water solvent extraction method is more suitable to study the metabolites of wax apple.

Key words: wax apple, gas chromatography-mass spectrometry, metabolites, metabonomics