关键词: 焦磷酸测序, PL3 基因, 基因编辑, 水稻

Abstract: Gene editing technology is developing rapidly, but there are few related detection methods. In order to find a detection method for gene-edited crops, this study used  editing sites of PL3 gene-edited rice as the target, effectively designed pyrosequencing amplification primers and sequencing primers, and conducted effectiveness testing, respectively using Sequence to Analyze and other programs as well as the two models of SNP and AQ, the qualitative and quantitative detection test of PL3 gene has been completed, and the pyrosequencing detection method on editing site of PL3 gene-edited rice  has been established. The results showed that the editing type rice could be distinguished from the wild type rice by detecting the editing sites based on pyrosequencing. Compared with the conventional methods, the method has the advantages of better accuracy, high efficiency and high sensitivity. It has a good application prospect in qualitative and quantitative detection of editing sites at the gene editing rice.

Key words: pyrosequencing, PL3 gene, gene editing, rice