关键词: 人类乳头瘤病毒, 宫颈癌, 核酸检测, 标准物质, 计量校准

Abstract: According to the global cancer statistics in 2018, the incidence of cervical cancer in China has ranked the second in the world, and it tends to occur at a younger age, posing a serious threat to women health. Research has shown that the infection of high-risk human papillomavirus (HPV) closely relates to the occurrence of cervical cancer. Hence, the detection of HPV is of great significance for the prevention and diagnosis of cervical cancer. The popular detection methods work based on PCR technology, which make qualitative and quantitative analysis of HPV nucleic acid. However, in the absence of uniform standards, it is difficult to ensure accurate and comparable measurement results. Reference material acts as a powerful tool for measurement and calibration, which could be applied to quality control of measurement process. This paper discussed the common detection methods for HPV and the influencing factors in the detection procedure, then analyzed the characteristics of different forms of reference materials for nucleic acid detection. It was expected to provide clues for the research and usage of the reference materials in HPV nucleic acid detection, which would be helpful for the scientific diagnosis of cervical cancer.

Key words: HPV, cervical cancer, nucleic acid detection, reference material, calibration