关键词: 干扰素&alpha, 2b, 制备, 表达, 纯品

Abstract: Recombinant human interferon α2b was prepared by cloning, expression, purification for obtaining rhIFN α2b with high activity and purity. Overlap extension PCR was performed to obtain the gene encoding for IFN α2b. A prokaryotic vector expressing rhIFN α2b was constructed successfully. The expression vector pBV220-IFN α2b was transformed into E. coli strain DH5α. After fermentation, the bacteria cells was collected and lysed. Finally, the rhIFN α2b was purified by denaturation, renaturation, ion exchange chromatography and gel filtration chromatography, and its specific activity reached 1×108 IU/mg. The results was expected to lay the foundation for preclinical research and long-acting preparation.

Key words: interferon α2b, preparation, expressing, purify